Collagen is the fundamental building block of muscles, tissues, tendons, and ligaments in mammals. It is also widely used in reconstructive and cosmetic surgery. Although scientists have a good understanding about how it behaves at the tissue-level, some key mechanical properties of collagen at the nanoscale still remain elusive. A recent experimental study conducted by researchers at the University of Illinois at Urbana-Champaign, Washington University, and Columbia University on nanoscale collagen fibrils reported on, previously unforeseen, reasons why collagen is such a resilient material.
Because one collagen fibril is about one millionth in size of the cross-section of a human hair, studying it requires equally small equipment. The group in the Department of Aerospace Engineering at U of I designed tiny devices--Micro-Electro-Mechanical Systems--smaller than one millimeter in size, to test the collagen fibrils.
A collagen fibril mounted on a MEMS mechanical testing device. At the bottom is a single human hair for size comparison.
Credit: University of Illinois Department of Aerospace Engineering
"Using MEMS-type devices to grip the collagen fibrils under a high magnification optical microscope, we stretched individual fibrils to learn how they deform and the point at which they break," said Debashish Das, a postdoctoral scholar at Illinois who worked on the project. "We also repeatedly stretched and released the fibrils to measure their elastic and inelastic properties and how they respond to repeated loading."
Das explained, "Unlike a rubber band, if you stretch human or animal tissue and then release it, the tissue doesn't spring back to its original shape immediately. Some of the energy expended in pulling it is dissipated and lost. Our tissues are good at dissipating energy-when pulled and pushed, they dissipate a lot of energy without failing.
This behavior has been known and understood at the tissue-level and attributed to either nanofibrillar sliding or to the gel-like hydrophilic substance between collagen fibrils. The individual collagen fibrils were not considered as major contributors to the overall viscoelastic behavior. But now we have shown that dissipative tissue mechanisms are active even at the scale of a single collagen fibril."
A very interesting and unexpected finding of the study is that collagen fibrils can become stronger and tougher when they are repeatedly stretched and let to relax.
"If we repeatedly stretch and relax a common engineering structure, it is more likely to become weaker due to fatigue," said U of I Professor Ioannis Chasiotis. "While our body tissues don't experience anywhere near the amount of stress we applied to individual collagen fibrils in our lab experiments, we found that after crossing a threshold strain in our cyclic loading experiments, there was a clear increase in fibril strength, by as much as 70 percent."
Das said the collagen fibrils themselves contribute significantly to the energy dissipation and toughness observed in tissues.
"What we found is that individual collagen fibrils are highly dissipative biopolymer structures. From this study, we now know that our body dissipates energy at all levels, down to the smallest building blocks. And properties such as strength and toughness are not static, they can increase as the collagen fibrils are exercised," Das said.
What's the next step? Das said with this new understanding of the properties of single collagen fibrils, scientists may be able to design better dissipative synthetic biopolymer networks for wound healing and tissue growth, for example, which would be both biocompatible and biodegradable.
The study "Energy dissipation in mammalian collagen fibrils: Cyclic strain-induced damping, toughening, and strengthening," was co-authored by Julia Liu, Debashish Das, Fan Yang, Andrea G. Schwartz, Guy M. Genin, Stavros Thomopoulos, and Ioannis Chasiotis. It is published in Acta Biomaterialia.
The research was supported by the National Science Foundation and National Institutes of Health and by the National Science Foundation Science and Technology Center for Engineering MechanoBiology. Das' effort was supported by a grant from the National Science Foundation.
Ioannis Chasiotis | EurekAlert!
Lung images of twins with asthma add to understanding of the disease
06.12.2019 | University of Western Ontario
Between Arousal and Inhibition
06.12.2019 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Texas and MIT researchers create virtual UAVs that can predict vehicle health, enable autonomous decision-making
In the not too distant future, we can expect to see our skies filled with unmanned aerial vehicles (UAVs) delivering packages, maybe even people, from location...
With ultracold chemistry, researchers get a first look at exactly what happens during a chemical reaction
The coldest chemical reaction in the known universe took place in what appears to be a chaotic mess of lasers. The appearance deceives: Deep within that...
Abnormal scarring is a serious threat resulting in non-healing chronic wounds or fibrosis. Scars form when fibroblasts, a type of cell of connective tissue, reach wounded skin and deposit plugs of extracellular matrix. Until today, the question about the exact anatomical origin of these fibroblasts has not been answered. In order to find potential ways of influencing the scarring process, the team of Dr. Yuval Rinkevich, Group Leader for Regenerative Biology at the Institute of Lung Biology and Disease at Helmholtz Zentrum München, aimed to finally find an answer. As it was already known that all scars derive from a fibroblast lineage expressing the Engrailed-1 gene - a lineage not only present in skin, but also in fascia - the researchers intentionally tried to understand whether or not fascia might be the origin of fibroblasts.
Fibroblasts kit - ready to heal wounds
Research from a leading international expert on the health of the Great Lakes suggests that the growing intensity and scale of pollution from plastics poses serious risks to human health and will continue to have profound consequences on the ecosystem.
In an article published this month in the Journal of Waste Resources and Recycling, Gail Krantzberg, a professor in the Booth School of Engineering Practice...
03.12.2019 | Event News
15.11.2019 | Event News
15.11.2019 | Event News
06.12.2019 | Earth Sciences
06.12.2019 | Life Sciences
06.12.2019 | Information Technology