Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New imaging technique ensures rapid profiling of autoantibodies in rheumatoid arthritis

26.10.2007
Using a new imaging technique, a fast and accurate profile of autoantibodies present in the blood serum of rheumatic patients can be made.

This profile can give valuable information about the progress of the disease. A unique feature of this so-called Surface Plasmon Resonance (SPR) technique is that it directly tests on blood serum, without complex preprocessing. A special chip will enable many parallel tests. Scientists from the University of Twente and the Radboud University Nijmegen, both in The Netherlands, will publish about the new imaging technique in the Journal of the American Chemical Society (JACS).

The scientists have run tests on the serum of 50 RA patients as well as a control group of 29 persons. Direct testing on blood serum is unique: in other techniques fluorescent labels and preprocessing is necessary to visualize the relevant proteins. The diluted serum is led over a special gold coated microchip containing a large number of spots with a specific peptide coating. Whenever these peptides interact with autoantibodies present in the serum, this process can be monitored by Surface Plasmon Resonance Imaging (SPR). Using laser light, all gold spots are scanned: the reflection of light of the spots changes whenever there is a molecular interaction on the spot. At a certain angle of light, there is no reflection at all: this is the so-called SPR dip undergoing a shift caused by the interaction. The technique goes beyond proving that autoantibodies are present: the interaction between the protein and the antibody can be monitored real-time and without any labels.

Autoantibodies are manufactured by the immune system as a reaction on the so-called citrullinated proteins playing a role in rheumatoid arthritis. On a single chip, several types of peptides can be placed, for rapid parallel screening. The next step is to investigate in what way the patient profiles help to monitor the progress of the disease. This could lead to more personalized treatment in the future. The applications are not limited to monitoring rheuma or other autoimmune diseases: SPR imaging can be used for monitoring a wide range of biomolecular interactions.

The research was led by dr. Richard Schasfoort of the BIOS Lab-on-a-chip group, part of the MESA+ Institute for Nanotechnology of the University of Twente. He has closely cooperated with the Biomolecular Chemistry group of the Radboud University Nijmegen, of Professor Ger Pruijn. The research has been financed by the Dutch Technology Foundation (STW) within a project called ‘Proteomics on a chip for monitoring autoimmune diseases’.

Wiebe van der Veen | alfa
Further information:
http://pubs.acs.org/journals/jacsat/index.html

More articles from Medical Engineering:

nachricht Can radar replace stethoscopes?
14.08.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg

nachricht Novel PET imaging method could track and guide therapy for type 1 diabetes
03.08.2018 | Society of Nuclear Medicine and Molecular Imaging

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Color effects from transparent 3D-printed nanostructures

New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference

Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

LaserForum 2018 deals with 3D production of components

17.08.2018 | Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

 
Latest News

Smallest transistor worldwide switches current with a single atom in solid electrolyte

17.08.2018 | Physics and Astronomy

Robots as Tools and Partners in Rehabilitation

17.08.2018 | Information Technology

Climate Impact Research in Hannover: Small Plants against Large Waves

17.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>