The cutting edge laser technology has helped to attract £1.3 million from the MRC (Medical Research Council) for a five-year project that will offer a new insight into the tiny world of activity taking place within single cells and could contribute to the design of new drugs to treat human diseases such as asthma and arthritis with fewer side effects.
The team, involving scientists from the University’s Schools of Biomedical Science (Professor Steve Hill and Dr Steve Briddon) and Pharmacy (Dr Barrie Kellam), is concentrating on a type of specialised docking site (receptor) on the surface of a cell that recognises and responds to a natural chemical within the body called adenosine.
These A3-adenosine receptors work within the body by binding with proteins to cause a response within cells and are found in very tiny and highly specialised area of a cell membrane called microdomains. Microdomains contain a collection of different molecules that are involved in telling the cell how to respond to drugs or hormones.
It is believed that these receptors play an important role in inflammation within the body and knowing more about how they operate could inform the future development of anti-inflammatory drugs that target just those receptors in the relevant microdomain of the cell, without influencing the same receptors in other areas of the cell. However, scientists have never before been able to look in detail at their activity within these tiny microscopic regions of a living cell.
The Nottingham researchers have solved this problem by creating novel drug molecules which have fluorescent labels attached. Using a cutting edge laser technology called fluorescence correlation spectroscopy, the fluorescent drug molecules can be detected as they glow under the laser beam of a highly sensitive microscope. This allows their binding to the receptor to be followed for the first time in real time at the single molecule level.
Leading the project, Professor Steve Hill in the School of Biomedical Sciences said: “These microdomains are so tiny you could fit five million on them on a full stop. There are 10,000 receptors on each cell, and we are able to follow how single drug molecules bind to individual receptors in these specialised microdomains.
“What makes this single molecule laser technique unique is that we are looking at them in real time on a living cell. Other techniques that investigate how drugs bind to their receptors require many millions of cells to get a big enough signal and this normally involves destroying the cells in the process”
The researchers will be using donated blood as a source of A3-receptors in specialised human blood cells (neutrophils) that have important roles during inflammation.
Different types of adenosine receptors are found all over the body and can exist in different areas of the cell membrane and have different properties. Scientists hope that eventually the new technology could also be used to unlock the secrets of the role they play in a whole host of human diseases.
The fluorescent molecules developed as part of the research project will also be useful in drug screening programmes and The University of Nottingham will be making these fluorescent drugs available to the wider scientific community through its links with its spin-out company CellAura Technologies Ltd.
Emma Thorne | alfa
New quantum material could warn of neurological disease
11.04.2019 | Purdue University
High-strength MRI tracks MS progression
09.04.2019 | Radiological Society of North America
A stellar flare 10 times more powerful than anything seen on our sun has burst from an ultracool star almost the same size as Jupiter
A localization phenomenon boosts the accuracy of solving quantum many-body problems with quantum computers which are otherwise challenging for conventional computers. This brings such digital quantum simulation within reach on quantum devices available today.
Quantum computers promise to solve certain computational problems exponentially faster than any classical machine. “A particularly promising application is the...
The technology could revolutionize how information travels through data centers and artificial intelligence networks
Engineers at the University of California, Berkeley have built a new photonic switch that can control the direction of light passing through optical fibers...
Physicists observe how electron-hole pairs drift apart at ultrafast speed, but still remain strongly bound.
Modern electronics relies on ultrafast charge motion on ever shorter length scales. Physicists from Regensburg and Gothenburg have now succeeded in resolving a...
Engineers create novel optical devices, including a moth eye-inspired omnidirectional microwave antenna
A team of engineers at Tufts University has developed a series of 3D printed metamaterials with unique microwave or optical properties that go beyond what is...
17.04.2019 | Event News
15.04.2019 | Event News
09.04.2019 | Event News
18.04.2019 | Life Sciences
18.04.2019 | Physics and Astronomy
18.04.2019 | Life Sciences