Kobielak and his team used a system to make all of the sweat gland cells in a mouse easy to spot: labeling them with green fluorescent protein (GFP), which is visible under ultraviolet light.
Staining of slow-cycling sweat gland cells (green) with the protein laminin (red) and the fluorescent stain DAPI (blue) (Image by Yvonne Leung)
Over time, the GFP became dimmer as it was diluted among dividing sweat gland cells. After four weeks, the only cells that remained fluorescent were the ones that did not divide or divided very slowly — a known property among stem cells of certain tissues, including the hair follicle and cornea. Therefore, these slow-dividing, fluorescent cells in the sweat gland’s coiled lower region were likely also stem cells.
Then, the first author of this paper, graduate student Yvonne Leung, tested whether these fluorescent cells could do what stem cells do best — differentiate into multiple cell types. To the researchers’ surprise, these glowing cells generated not only sweat glands, but also hair follicles when placed in the skin of a mouse without GFP.
The researchers also determined that under certain conditions, the sweat gland stem cells could heal skin wounds and regenerate all layers of the epidermis.
“That was a big surprise for us that those very quiescent sweat gland stem cells maintain multilineage plasticity — participating not only in their own regeneration, but also in the regeneration of hair follicles and skin after injury,” said Kobielak, assistant professor of pathology at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC.
This offers exciting possibilities for developing future stem cell-based treatments for skin and sweat gland-related conditions, such as hyperhidrosis or hypohidrosis (excessive or insufficient sweating). It could also lay the foundation for creating fully functional skin — containing both sweat glands and hair follicles — for burn victims.
Additional co-authors on the study were Eve Kandyba, Yi-Bu Chen and Seth Ruffins from the Broad Center.
The study was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health (grant numbers R03-AR061028 and R01-AR061552).
Marie Rippen | EurekAlert!
Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY
NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation
A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.
The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
20.07.2018 | Power and Electrical Engineering
20.07.2018 | Information Technology
20.07.2018 | Materials Sciences