The winter sun feels welcome, but not so a summer sunburn. Research over the past 20 years has shown that proteins on the surface of nerve cells enable the body to sense several different temperatures. Now scientists have discovered how just a few of these proteins, called ion channels, distinguish perhaps dozens of discrete temperatures, from mildly warm to very hot.
Researchers showed that the building blocks, or subunits, of heat-sensitive ion channels can assemble in many different combinations, yielding new types of channels, each capable of detecting a different temperature. The discovery, in cell cultures, demonstrates for the first time that only four genes, each encoding one subunit type, can generate dozens of different heat-sensitive channels.
"Researchers in the past have assumed that because there are only four genes, there are only four heat-sensitive channels, but now we have shown that there are many more," said Jie Zheng, leader of the research and an associate professor of physiology and membrane biology at the UC Davis School of Medicine.
The research publishes in the Journal of Biological Chemistry on March 2.
Ion channels are pores in cell membranes. Their ability to open and close controls the flow of charged ions, which turns neuron signalling on or off -- in this case to inform the body of the temperature the neuron senses.
The researchers found that when different subunits combine, the resultant hybrid, or heteromeric, channel can detect temperatures about midway between what the "parent" channels detect.
One of the channels they studied, called TRPV1, reacts to hot temperatures -- about 100 degrees Fahrenheit. It is also responsible for the ability to sense spicy foods, such as chili peppers. A second channel, TRPV3, responds to temperatures of about 85 degrees. It also senses many food flavors such as those found in rosemary, oregano, vanilla and cinnamon that elicit a warm sensation.
When the TRPV1 and TRPV3 subunits recombine, the heteromeric channel is tuned to about 92 degrees. Surprisingly, the study showed that the hybrid channel has an even higher chemical sensitivity than the channels that made it up.
Zheng and his colleagues also showed that channels made up of TRPV1 and TRPV3 subunits react to heat at a rate about midway between that of the two constituent channel subunits. But repeatedly exposing the hybrid channels to their target temperature boosted their response, a behavior called sensitization, which TRPV3 also exhibits.
"It says 'I remember this temperature. I will make a really loud noise to tell the system that it is coming,'" Zheng said. The process allows the body to be more sensitive to temperature.
By contrast, TRPV1 typically responds the same way when repeatedly exposed to its target temperature -- and sometimes even decreases its response, a process called desensitization. It helps the body to adapt to high temperature, Zheng explained.
The research builds on work the team published in 2007 demonstrating that the heat-sensitive subunits can combine to form heteromeric channels. However, at the time, scientists didn't know how these channels respond to heat. The new work shows that the channels are indeed sensitive to different temperatures.
"Knowing that there are many distinct heat-sensing ion channels now opens the way to understand how neurons encode precise temperature information, an important process that allows us to enjoy the rich spectrum of temperature in life -- a memorable warm handshake, a soothing shower and a cup of hot latte -- and add vanilla flavor, please," Zheng said. "It also may provide insights regarding the causes and potential treatments for temperature-sensitivity disorders, such as Raynaud's syndrome."
Raynaud's syndrome is a condition that causes some areas of the body -- such as fingers, toes, the tip of the nose and ears -- to feel numb and cool in response to cold temperatures or stress. The cause is unknown.
The scientists introduced the genes for TRPV1 and TRPV3 channel subunits to cultured human kidney cells. They tagged the genes with fluorescent markers to confirm when the resulting proteins had combined to form a new channel complex.
Once functional channels were formed, the researchers used a glass pipette with a very fine tip to record ion channels' responses to temperature changes.
In order to rapidly increase the temperature, they built an apparatus that allowed them to deliver an infrared laser beam to the cell. The method allowed them to heat the channel more than a thousand times faster than commercially available heating devices.
The collaborative research is funded by the National Institutes of Health, the American Heart Association and the Chinese government.
The UC Davis School of Medicine is among the nation's leading medical schools, recognized for its research and primary-care programs. The school offers fully accredited master's degree programs in public health and in informatics, and its combined M.D.-Ph.D. program is training the next generation of physician-scientists to conduct high-impact research and translate discoveries into better clinical care. Along with being a recognized leader in medical research, the school is committed to serving underserved communities and advancing rural health. For more information, visit UC Davis School of Medicine at medschool.ucdavis.edu.
Carole Gan | EurekAlert!
A novel synthetic antibody enables conditional “protein knockdown” in vertebrates
20.08.2018 | Technische Universität Dresden
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
There are currently great hopes for solid-state batteries. They contain no liquid parts that could leak or catch fire. For this reason, they do not require cooling and are considered to be much safer, more reliable, and longer lasting than traditional lithium-ion batteries. Jülich scientists have now introduced a new concept that allows currents up to ten times greater during charging and discharging than previously described in the literature. The improvement was achieved by a “clever” choice of materials with a focus on consistently good compatibility. All components were made from phosphate compounds, which are well matched both chemically and mechanically.
The low current is considered one of the biggest hurdles in the development of solid-state batteries. It is the reason why the batteries take a relatively long...
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
20.08.2018 | Information Technology
20.08.2018 | Life Sciences
20.08.2018 | Information Technology