Using mathematical model, UC San Diego researchers devise optimal human stem cell culture
Human pluripotent stem cells (hPSCs) possess the ability to grow into almost any kind of cell, which has made them dynamic tools for studying early human development and disease, but much depends upon what they grow up in.
Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine used a powerful statistical tool called "design of experiments" or DOE to determine the optimal cell culture formula to grow and produce hPSCs.
"Currently, there are different culture methods and media that are not optimized or even chemically defined. There are several factors that may affect the growth of stem cells based on batch-to-batch media variation," said Alysson Muotri, PhD, associate professor in the UC San Diego departments of Pediatrics and Cellular and Molecular Medicine. "This affects science in many ways. For example, it slows down progress because conditions may not be pristine. It also makes it difficult for other labs to validate data because the media they use will likely not be the same as in the original experiments."
Muotri and colleagues used DOE to measure two critical growth factors used in hPSC media: basic fibroblast growth factor (bFGF) and neuregulin-1 beta 1 (NRG-1 beta 1). DOE is often used in scientific endeavors to measure and account for variations in data, but not so much in biology, said Muotri.
"If you ask a biology student what is the ideal temperature and pH for an enzyme, he/she will try to determine the best temperature in one experiment and the best pH in another experiment. Then, the student will erroneously conclude that these represent the optimal temperature and pH," said Muotri. "What is missing is the interaction between temperature and pH. The best working temperature may not be the most optimal pH condition. DOE takes into account positive, negative or neutral interactions between multiple factors at the same time."
Building upon earlier work, which had analyzed hundreds of other factors in hPSC media, the researchers determined the best formulations for bFGF and NRG-1 beta 1. They noted, however, that their findings are not fixed. "If science discovers a new factor that affects hPSC proliferation, we can add it into our DOE matrix to quickly test and re-formulate the media," said Muotri.
The researchers hope their findings will lead to a new standard for hPSC cultures. "Any lab in the world can have access to the same formulation, with no variability," said Muotri. "We also think this method could be applied towards the development of culture conditions during differentiation of human stem cells. Ideally, we want to create transition media formulations that subtly change during cell type specialization, mimicking the human embryo."
Muotri said his team is working with the UC San Diego Technology Transfer Office to find industry partners to assist in making the new technology accessible to all laboratories using hPSCs.
Co-authors include Paulo A. Marinho and Thanathom Chailangkarn, UCSD Department of Pediatrics/Rady Children's Hospital-San Diego, Department of Cellular and Molecular Medicine and UCSD Stem Cell Program.
Funding for this research came, in part, from the California Institute for Regenerative Medicine and the National Institutes of Health (1-DP2-OD006495-1).
Scott LaFee | EurekAlert!
World’s Largest Study on Allergic Rhinitis Reveals new Risk Genes
17.07.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Plant mothers talk to their embryos via the hormone auxin
17.07.2018 | Institute of Science and Technology Austria
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
17.07.2018 | Information Technology
17.07.2018 | Materials Sciences
17.07.2018 | Power and Electrical Engineering