Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

TGen analysis identifies biomarkers for diabetic kidney failure

18.12.2009
Pooled DNA technique identifies variances that could indicate susceptibility to end-stage renal disease

Researchers using a DNA analysis tool developed by the Translational Genomics Research Institute (TGen) and UCLA have identified genetic markers that could help treat chronic kidney disease among diabetics.

Study results, published in the December edition of Diabetic Medicine, show it is possible to identify biomarkers associated with end-stage renal disease (ESRD) from the pooled DNA of more than 1,000 diabetics. Specifically, TGen researchers identified genes that could potentially contribute to ESRD among those with Type 1 Diabetes.

ESRD almost always follows chronic kidney failure and although treatable with dialysis or transplantation, mortality rates remain high. While diabetic kidney disease is one of the most common complications of diabetes, it is currently not possible to determine who is at risk for ESRD.

“Identification of specific DNA variants may enhance our understanding of genetic risk factors for renal disease and may provide diagnostic value in determining which patients are at greatest risk of developing ESRD,” said Dr. Johanna DiStefano, Director of TGen’s Diabetes, Cardiovascular & Metabolic Diseases Division and the paper’s senior author.

The need to rapidly identify individuals with a predisposition to ESRD and to discover new drugs to prevent and treat this devastating condition is becoming critical, as the average onset of this disease affects ever-younger populations. Although Type 1 diabetes is distinctly different from Type 2 diabetes, the development of kidney disease is similar in individuals with either form. Researchers expect the current findings to impact individuals with both forms of the disease.

Nearly 23 percent of Americans diagnosed with ESRD die within the first year. And the annual cost for the nearly 400,000 people who require blood dialysis or kidney transplants is more than $16 billion.

TGen researchers tested the pooled genomic DNA from more than 500 cases of those with ESRD and compared that to more than 500 patients who had Type I diabetes for at least 20 years with no sign of ESRD. Scientists performed a whole genome association scan to look for single nucleotide polymorphisms (SNPs), which are the individual letters of DNA that vary among individuals. They sought SNPs that indicate a susceptibility to ESRD.

Experiments identified at least eight locations along the nearly 3-billion-base human genome that are ripe for further investigation of their ties to ESRD, according to the paper, Genome-wide SNP genotyping study using pooled DNA to identify candidate markers mediating susceptibility to end-stage renal disease attributed to Type 1 diabetes.

The study found at least six markers that may be associated with Type 1 diabetes, a lifelong disease that occurs when the pancreas does not produce enough insulin to properly control blood-sugar levels. There currently is no way to prevent Type 1, which is caused by autoimmune, genetic or environmental factors.

The study found two markers that may be associated with Type 2 diabetes, the most common form of diabetes. Type 2 develops when the body fails to produce enough insulin, which helps turn glucose from the sugars and starches we eat into energy. The failure to process glucose starves cells of energy and over time can damage the eyes, nerves, heart or kidneys. Type 2 diabetes can be prevented or treated with proper diet and exercise.

Using a similar pooled DNA analysis, TGen researchers, working with collaborators at the University of California Los Angeles, last year developed a way to identify an individual’s DNA from among a mixture of DNA of hundreds or even thousands of individuals.

“This technique will increasingly be used in identifying the genetic origins of many Types of disease,’’ predicted Dr. David Craig, Associate Director of TGen’s Neurogenomics Division, who co-developed the technique and was lead author of the current study.

Diabetic nephropathy, or kidney disease, is the most common cause of ESRD. It occurs when chronic kidney failure has progressed to the point when either dialysis or kidney transplants are required to prevent blood poisoning.

Nearly 24 million Americans, about 8 percent, have diabetes, according to the American Diabetes Association, which lists the disease as the nation’s seventh leading cause of death.

About TGen
The Translational Genomics Research Institute (TGen) is a Phoenix, Arizona-based non-profit organization dedicated to conducting groundbreaking research with life changing results. Research at TGen is focused on helping patients with diseases such as cancer, neurological disorders and diabetes. TGen is on the cutting edge of translational research where investigators are able to unravel the genetic components of common and complex diseases. Working with collaborators in the scientific and medical communities, TGen believes it can make a substantial contribution to the efficiency and effectiveness of the translational process. TGen is affiliated with the Van Andel Research Institute in Grand Rapids, Michigan. For more information, visit: www.tgen.org.
Press Contact:
Steve Yozwiak
TGen Senior Science Writer
602-343-8704
syozwiak@tgen.org

Steve Yozwiak | EurekAlert!
Further information:
http://www.tgen.org/news/index.cfm?newsid=1734
http://www.tgen.org

More articles from Life Sciences:

nachricht Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg

nachricht Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: UNH scientists help provide first-ever views of elusive energy explosion

Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.

Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Purdue cancer identity technology makes it easier to find a tumor's 'address'

16.11.2018 | Health and Medicine

Good preparation is half the digestion

16.11.2018 | Life Sciences

Microscope measures muscle weakness

16.11.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>