Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Structure of a mitochondrial ATP synthase

19.11.2019

ATP synthase is a universal molecular machine for energy conversion. By coupling to cellular respiration in mitochondria, it catalyzes conversion of chemical energy of cells.

Mitochondrial ATP synthase is composed of dimers that, when come together, form membrane curvature that is essential for efficient energy conversion. While the mitochondrial signature lipid cardiolipin and its interactions with proteins are believed to contribute to this process, it was not directly visualized before. In addition, it was unclear to what extent the ATP synthase has diverged across different species.

Alexander Mühleip from Amunts lab used the single-cell photosynthetic organism Euglena gracilis, which belongs to a phylum that also includes human parasites, to extract the mitochondrial ATP synthase. Its structure was then determined using cryo-EM, allowing the reconstruction of the atomic model.


This is an everview of the E. gracilis ATP synthase dimer.

Credit: A. Muhleip

The high resolution of the cryo-EM density map allowed identification of 29 different protein subunits and 25 cardiolipin molecules. Some of the cardiolipins appear to modulate the critical channel for proton transfer that fuels the machine, which is the first evidence for their direct involvement.

The model shows that the Euglena mitochondrial ATP synthase is highly divergent with 13 protein subunits being specific to this system. The new subunits contribute to the formation of dimers glued by lipids, providing experimental evidence that the key functional property of membrane curvature induction has likely occurred independently in evolution.

Furthermore, a membrane-embedded subcomplex was found at the periphery of the ATP synthase, displacing the membrane. Sarah McComas performed molecular dynamics simulations supporting that the newly found subcomplex contributes to the membrane curvature.

The atomic model of the mitochondrial ATP synthase with native lipids provides a new perspective for its functional analysis. The different type of molecular organization of this essential energy production machinery re-establishes the defining features of the complex and opens the door for reconstructing the evolution of the mitochondrial ATP synthase. Finally, identified similarities to the human parasites provide a new perspective for a therapeutic exploration.

Amunts' lab page

###

About SciLifeLab

SciLifeLab is a joint enterprise of Swedish universities, that aims to provide frontline technologies for the Swedish academic community and develop cutting-edge research programs. Situated on the expanding Stockholm biomedical campus, SciLifeLab offers the opportunity to work in an internationally competitive and synergistic environment. The center combines technical expertise with advanced knowledge of translational medicine and molecular bioscience.

Vasileios Kyriakidis | EurekAlert!
Further information:
http://dx.doi.org/10.7554/eLife.51179

More articles from Life Sciences:

nachricht Turning carbon dioxide into liquid fuel
06.08.2020 | DOE/Argonne National Laboratory

nachricht Tellurium makes the difference
06.08.2020 | Friedrich-Schiller-Universität Jena

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: ScanCut project completed: laser cutting enables more intricate plug connector designs

Scientists at the Fraunhofer Institute for Laser Technology ILT have come up with a striking new addition to contact stamping technologies in the ERDF research project ScanCut. In collaboration with industry partners from North Rhine-Westphalia, the Aachen-based team of researchers developed a hybrid manufacturing process for the laser cutting of thin-walled metal strips. This new process makes it possible to fabricate even the tiniest details of contact parts in an eco-friendly, high-precision and efficient manner.

Plug connectors are tiny and, at first glance, unremarkable – yet modern vehicles would be unable to function without them. Several thousand plug connectors...

Im Focus: New Strategy Against Osteoporosis

An international research team has found a new approach that may be able to reduce bone loss in osteoporosis and maintain bone health.

Osteoporosis is the most common age-related bone disease which affects hundreds of millions of individuals worldwide. It is estimated that one in three women...

Im Focus: AI & single-cell genomics

New software predicts cell fate

Traditional single-cell sequencing methods help to reveal insights about cellular differences and functions - but they do this with static snapshots only...

Im Focus: TU Graz Researchers synthesize nanoparticles tailored for special applications

“Core-shell” clusters pave the way for new efficient nanomaterials that make catalysts, magnetic and laser sensors or measuring devices for detecting electromagnetic radiation more efficient.

Whether in innovative high-tech materials, more powerful computer chips, pharmaceuticals or in the field of renewable energies, nanoparticles – smallest...

Im Focus: Tailored light inspired by nature

An international research team with Prof. Cornelia Denz from the Institute of Applied Physics at the University of Münster develop for the first time light fields using caustics that do not change during propagation. With the new method, the physicists cleverly exploit light structures that can be seen in rainbows or when light is transmitted through drinking glasses.

Modern applications as high resolution microsopy or micro- or nanoscale material processing require customized laser beams that do not change during...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“Conference on Laser Polishing – LaP 2020”: The final touches for surfaces

23.07.2020 | Event News

Conference radar for cybersecurity

21.07.2020 | Event News

Contact Tracing Apps against COVID-19: German National Academy Leopoldina hosts international virtual panel discussion

07.07.2020 | Event News

 
Latest News

Rare Earth Elements in Norwegian Fjords?

06.08.2020 | Earth Sciences

Anode material for safe batteries with a long cycle life

06.08.2020 | Power and Electrical Engineering

Turning carbon dioxide into liquid fuel

06.08.2020 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>