Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New stem cell tools to aid drug development

03.09.2008
Scientists have designed, developed and tested new molecular tools for stem cell research to direct the formation of certain tissue types for use in drug development programmes

A collaborative team of scientists from Durham University and the North East England Stem Cell Institute (NESCI) have developed two synthetic molecules which can be used to coax stem cells to ‘differentiate’ - that is, transform into other forms of tissue.

Their use could also help reduce the number of animals used in laboratory research.

The team’s results are published in the current issue of the scientific journal, Organic and Biomolecular Chemistry.

The new molecules, called EC23 and EC19*, have been found in robust scientific tests to be far more stable than the naturally-occurring molecule currently used to induce stem cells to differentiate in the laboratory, known as All-trans-retinoic Acid (ATRA). Their use will thus improve the reliability of experiments.

The scientists, who tested the effectiveness of EC23 and EC19 on four types of stem cells, say it is also significant that each individual synthetic molecule has been found to be more effective at causing the cells to transform into specific types of tissue.

For example, EC23 was found to be particularly effective at producing neurons (nerve cells) which can be used in laboratory testing for drugs for brain disorders such as Alzheimer’s Disease and Parkinson’s Disease.

In contrast, EC19 was found to be particularly effective at producing epithelial cells – the cells that line the inner and outer surfaces of the body.

Stem cells are a special type of cell that has the ability to renew other cells in the body. One of the challenges facing stem cell scientists is to find out how these may be re-programmed to become different tissue types.

The team consisted of synthetic chemists, Dr Andrew Whiting and Professor Todd Marder and stem cell biologist, Dr Stefan Przyborski and their research groups at Durham University, who are all members of the North East England Stem Cell Institute (NESCI). They also worked with spin-out company Reinnervate; local SME, High Force Research Ltd and Newcastle University.

Dr Przyborski said: “The key thing about these synthetic molecules is that they remain stable and are exactly the same every time you use them, ensuring more reliable scientific experiments compared to those which use ATRA. Because the results will be more scientifically robust, this will accelerate drug development using human stem cell-derived tissues and potentially reduce the numbers of animals used in such research”

“Another significant characteristic of these synthetic molecules is that they direct stem cells down specific pathways, meaning that they, individually, will be useful for very specific types of drug development work. EC23, for example, produces almost 40 per cent more neurons than ATRA.”

Dr Whiting said: "We've set out to make stable mimics of natural compounds which control cell development, but in this case, not only have we uncovered a compound which is not only stable and does what the natural system does, but it actually seems to be better as well. It’s a real bonus and shows the validity of the approach."

Dr Przyborski, who is also Director and Chief Scientific Officer of spin-out company Reinnervate Limited, is currently marketing EC23 through Reinnervate. He said the results showed that synthetic retinoids EC23 and EC19 could be used to replace All-trans-retinoic acid (ATRA).

ATRA is sensitive to light, heat and air, and exposure to light especially causes it to degrade rapidly, meaning scientists are never sure exactly what concentration or what mixture of isomers they are working with.

The experiments on EC23 and EC19, which included highly detailed analysis by Nuclear Magnetic Resonance (NMR) showed that these synthetic molecules were not sensitive to light and therefore did not degrade.

The scientists are now developing a ‘molecular toolkit’ of synthetic compounds which are tailor made for specific stem cell and drug development work.

The project was funded in part by Reinnervate, the regional development agency, One NorthEast, Durham University, High Force Research Ltd., NESCI, and by research councils the MRC, EPSRC, BBSRC.

The close collaboration between chemists and biologists is critical to achieving this type of breakthrough.

Via a related collaboration with Durham University polymer chemist, Professor Neil Cameron, Reinnervate is also marketing a unique plastic scaffold to drug developers, which allows stem cells and other tissue to be grown in the laboratory in conditions similar to the way they grow in the human body. Extensive tests have shown the technology is a cheap and straightforward way of cultivating cells in three-dimensional forms.

* In the scientific paper, EC23 and EC19 are referred to as 4a and 4b respectively.

Claire Whitelaw | alfa
Further information:
http://www.durham.ac.uk

More articles from Life Sciences:

nachricht Turning carbon dioxide into liquid fuel
06.08.2020 | DOE/Argonne National Laboratory

nachricht Tellurium makes the difference
06.08.2020 | Friedrich-Schiller-Universität Jena

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: ScanCut project completed: laser cutting enables more intricate plug connector designs

Scientists at the Fraunhofer Institute for Laser Technology ILT have come up with a striking new addition to contact stamping technologies in the ERDF research project ScanCut. In collaboration with industry partners from North Rhine-Westphalia, the Aachen-based team of researchers developed a hybrid manufacturing process for the laser cutting of thin-walled metal strips. This new process makes it possible to fabricate even the tiniest details of contact parts in an eco-friendly, high-precision and efficient manner.

Plug connectors are tiny and, at first glance, unremarkable – yet modern vehicles would be unable to function without them. Several thousand plug connectors...

Im Focus: New Strategy Against Osteoporosis

An international research team has found a new approach that may be able to reduce bone loss in osteoporosis and maintain bone health.

Osteoporosis is the most common age-related bone disease which affects hundreds of millions of individuals worldwide. It is estimated that one in three women...

Im Focus: AI & single-cell genomics

New software predicts cell fate

Traditional single-cell sequencing methods help to reveal insights about cellular differences and functions - but they do this with static snapshots only...

Im Focus: TU Graz Researchers synthesize nanoparticles tailored for special applications

“Core-shell” clusters pave the way for new efficient nanomaterials that make catalysts, magnetic and laser sensors or measuring devices for detecting electromagnetic radiation more efficient.

Whether in innovative high-tech materials, more powerful computer chips, pharmaceuticals or in the field of renewable energies, nanoparticles – smallest...

Im Focus: Tailored light inspired by nature

An international research team with Prof. Cornelia Denz from the Institute of Applied Physics at the University of Münster develop for the first time light fields using caustics that do not change during propagation. With the new method, the physicists cleverly exploit light structures that can be seen in rainbows or when light is transmitted through drinking glasses.

Modern applications as high resolution microsopy or micro- or nanoscale material processing require customized laser beams that do not change during...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“Conference on Laser Polishing – LaP 2020”: The final touches for surfaces

23.07.2020 | Event News

Conference radar for cybersecurity

21.07.2020 | Event News

Contact Tracing Apps against COVID-19: German National Academy Leopoldina hosts international virtual panel discussion

07.07.2020 | Event News

 
Latest News

Anode material for safe batteries with a long cycle life

06.08.2020 | Power and Electrical Engineering

Turning carbon dioxide into liquid fuel

06.08.2020 | Life Sciences

Tellurium makes the difference

06.08.2020 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>