A paper was published by Kazan Federal University in the Journal of Structural Biology
Project leader, Head of Structural Biology Lab Konstantin Usachev explains, "One of the main factors favoring a microorganism's survival in extreme conditions is preserving ribosomes - a macromolecular complex comprising RNA and proteins.
For this purpose, a cell synthesizes special proteins which may stop translating ribosomes until the stress is over. One of such proteins is hibernation promoting factor (HPF) which transfers ribosomes to the 'hibernation state'."
Staphylococcus aureus is one of the most dangerous bacteria for humans, a pathogen causing many nosocomial infections. The danger is both in the staph's virulence and antibiotic resistance. There are currently no specific anti-staphylococcus antibiotics. That's why it's so important to seek new targets for antibiotic therapy in the bacteria.
"Using high-resolution (1,6 Å) X-ray structure analysis, we solved the structure of the domain of HPF from Staphylococcus aureus, which regulates the dimerization of ribosomes and keeps in their 'hibernation state'.
Based on the obtained information, we found the key amino acid residues which provide for the functioning of the protein and used genetic engineering to produce mutated forms of this protein by single amino acid residues substitutions. After that, the mutated forms were analyzed for their capabilities of ribosome hibernation, and we found five residues essential for the functioning of the protein.
Our results open the way to exploring new compounds that could bond with this protein areas and decrease the vitality of Staphylococcus aureus under stress," adds Dr. Usachev.
This was the first protein structure solved by X-ray analysis implemented with the new XtaLab Synergy S diffractometer; it was installed at the Structural Biology Lab this past May.
"Our new diffractometer has a sensitive detector, which allows analyzing the structure of large biomolecules in a monocrystal form, such as proteins and nucleic acids," concludes Dr. Usachev.
The Structural Biology Lab currently cooperates with the Chemoinformatics and Molecular Modelling Lab in searching the databases for new compounds which can bind with the Staph stress proteins.
Yury Nurmeev | EurekAlert!
TU Bergakademie Freiberg researches virus inhibitors from the sea
27.03.2020 | Technische Universität Bergakademie Freiberg
The Venus flytrap effect: new study shows progress in immune proteins research
27.03.2020 | Jacobs University Bremen gGmbH
Together with their colleagues from the University of Würzburg, physicists from the group of Professor Alexander Szameit at the University of Rostock have devised a “funnel” for photons. Their discovery was recently published in the renowned journal Science and holds great promise for novel ultra-sensitive detectors as well as innovative applications in telecommunications and information processing.
The quantum-optical properties of light and its interaction with matter has fascinated the Rostock professor Alexander Szameit since College.
Researchers at the University of Zurich show that different stem cell populations are innervated in distinct ways. Innervation may therefore be crucial for proper tissue regeneration. They also demonstrate that cancer stem cells likewise establish contacts with nerves. Targeting tumour innervation could thus lead to new cancer therapies.
Stem cells can generate a variety of specific tissues and are increasingly used for clinical applications such as the replacement of bone or cartilage....
An international research team led by Kiel University develops an extremely porous material made of "white graphene" for new laser light applications
With a porosity of 99.99 %, it consists practically only of air, making it one of the lightest materials in the world: Aerobornitride is the name of the...
Researchers at Graz University of Technology have developed a framework by which wireless devices with different radio technologies will be able to communicate directly with each other.
Whether networked vehicles that warn of traffic jams in real time, household appliances that can be operated remotely, "wearables" that monitor physical...
Terahertz waves are becoming ever more important in science and technology. They enable us to unravel the properties of future materials, test the quality of...
26.03.2020 | Event News
23.03.2020 | Event News
03.03.2020 | Event News
27.03.2020 | Power and Electrical Engineering
27.03.2020 | Life Sciences
27.03.2020 | Life Sciences