Researchers at the Max Planck Florida Institute for Neuroscience and Kanazawa University, Japan, have succeeded in imaging structural dynamics of living neurons with an unprecedented spatial resolution
Imaging structural dynamics of living cells and neurons
While progress has been made over the past decades in the pursuit to optimize atomic force microscopy (AFM) for imaging living cells, there were still a number of limitations and technological issues that needed to be addressed before fundamental questions in cell biology could be address in living cells.
In their March publication in Scientific Reports, researchers at Max Planck Florida Institute for Neuroscience and Kanazawa University describe how they have built the new AFM system optimized for live-cell imaging.
The system differs in many ways from a conventional AFM: it uses an extremely long and sharp needle attached to a highly flexible plate. The system is also optimized for fast scanning to capture dynamic cellular events. These modifications have enabled researchers to image living cells, such as mammalian cell lines or mature hippocampal neurons, without any sign of cellular damage.
"We've now demonstrated that our new AFM can directly visualize nanometer-scale morphological changes in living cells", explained Dr. Yasuda, neuroscientist and scientific director at the Max Planck Florida Institute for Neuroscience.
In particular, this study demonstrates the capability to track structural dynamics and remodeling of the cell surface, such as morphogenesis of filopodia, membrane ruffles, pit formation or endocytosis, in response to environmental stimulants. An example of this capability can be visualized in movie 1, where a fibroblast is imaged before and after treatment with insulin hormone, which intensely enhances the ruffling at the leading edge of the cell. Another example is seen in movie 2, where the morphological changes of a finger-like neuronal protrusion in the mature hippocampal neuron are observed.
According to Dr. Yasuda, the successful observations of structural dynamics in live neurons present the possibility of visualizing the morphology of synapses at nanometer resolution in real time in the near future. Since morphology changes of synapses underlie synaptic plasticity and our learning and memory, this will provide us with many new insights into mechanisms of how neurons store information in their morphology, how it changes synaptic strength and ultimately how it creates new memory.
Link to publication in Scientific Reports: http://www.
About Max Planck Florida Institute for Neuroscience
The Max Planck Florida Institute for Neuroscience (Jupiter, Florida, USA) specializes in the development and application of novel technologies for probing the structure, function and development of neural circuits. It is the first research institute of the Max Planck Society in the United States.
Jennifer Gutierrez | EurekAlert!
Further reports about: > AFM > Neuroscience > atomic force microscopy > atomic resolution > eukaryotic cells > image acquisition > light wavelength > live-cell imaging > living cells > morphological > morphological changes > morphology > nanoscale > neural circuits > neurons > spatial resolution > surface topography > synapses > synaptic
World’s Largest Study on Allergic Rhinitis Reveals new Risk Genes
17.07.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Plant mothers talk to their embryos via the hormone auxin
17.07.2018 | Institute of Science and Technology Austria
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
17.07.2018 | Information Technology
17.07.2018 | Materials Sciences
17.07.2018 | Power and Electrical Engineering