The study, carried out by the Departments of Radiology at the University of California and the Technical University of Munich, is freely available on-line at http://www.ingentaconnect.com/content/cog/ct ).
"We found that hESC-CMs labeled with ICG show a significant fluorescence up to 48 hours and can be visualized with optical imaging," said study lead author Dr. Sophie E. Boddington of the UCSF Department of Radiology. "Once more, the labeling procedure did not impair the viability or functional integrity of the cells."
According to the researchers, finding an efficient stem cell delivery technique has been a challenge. Too often, stem cells aimed at myocardial regeneration are "washed away" into noncardiac tissues or they soon die. A 10 percent survival rate for hESC-CMs has been common. Researchers knew that a better way to visualize the transplanted stem cells was needed to help direct and order stem cell delivery, and they also knew that materials used to label the cells had to be maximally fluorescent but not detrimental to the viability of the cells.
"We found the ICG-labeling technique advantageous because it is cost effective, straightforward and rapid," concluded Dr. Boddington. "Its most noticeable advantage is that it can be used with iron oxide-based nanoparticles for use with MRIs, as it increases single cell sensitivity. This is important because of the unpredictable migrational dynamics of the cells."
The researchers added that although PET techniques are more single cell sensitive than MRI, PET introduces radiation exposure and associated risks. In addition to not involving ionized radiation, optical imaging, using the MRI is quicker, less expensive, easier to perform, and noninvasive. Once more, ICG marking is already FDA-approved and accessible.
An added benefit, said Dr. Boddington, is that ICG is rapidly excreted, which suggests safe elimination.
While the ability of hESCs to regenerate into cells of almost all human tissues has been documented, and this provides a promising therapeutic source for myocardial regeneration after cardiac ischema, few studies have actually demonstrated this potential. Visualizing transplanted stem cells could, however, benefit cell retention and improve cell functional outcomes.
"Our data suggests that hESCs labeled with ICG may be useful in assessing delivery routes to improve the engraftment of transplanted hESC-CMs or other stem cell progenitors," concluded Dr. Boddington.
The study has significance, said Dr. Dwaine Emerich, section editor for Cell Transplantation.
"These studies are an important step forward in the quest for techniques to label and visualize stem cells in vivo," said Dr. Emerich. "The ICG-labeling technique described by Boddington and colleagues provides several advantages including its straight forward simplicity, reliability, and the fact that the technique can be easily combined with other developing imaging technologies."
Contact: Dr. Sophie E. Boddington, Department of Radiology, University of California, San Francisco, 185 Berry St. Suite 350, San Francisco, California 94107-0946. Tel. 415-353-4854 Fax 415-353-9425 Email: Sophie.Boddington@radiology.ucsf.edu
The editorial offices for CELL TRANSPLANTATION are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida and the Diabetes Research Institute, University of Miami Miller School of Medicine. Contact, David Eve, PhD. at firstname.lastname@example.org or Camillo Ricordi, MD at email@example.com
News Release by Randolph Fillmore, ScienceScribe.Net
David Eve | EurekAlert!
Genome Duplication Drives Evolution of Species
25.09.2018 | Universität Zürich
Why it doesn’t get dark when you blink
25.09.2018 | Deutsches Primatenzentrum GmbH - Leibniz-Institut für Primatenforschung
The Fraunhofer FEP has been involved in developing processes and equipment for cleaning, sterilization, and surface modification for decades. The CleanHand Network for development of systems and technologies to clean surfaces, materials, and objects was established in May 2018 to bundle the expertise of many partnering organizations. As a partner in the CleanHand Network, Fraunhofer FEP will present the Network and current research topics of the Institute in the field of hygiene and cleaning at the parts2clean trade fair, October 23-25, 2018 in Stuttgart, at the booth of the Fraunhofer Cleaning Technology Alliance (Hall 5, Booth C31).
Test reports and studies on the cleanliness of European motorway rest areas, hotel beds, and outdoor pools increasingly appear in the press, especially during...
The building blocks of matter in our universe were formed in the first 10 microseconds of its existence, according to the currently accepted scientific picture. After the Big Bang about 13.7 billion years ago, matter consisted mainly of quarks and gluons, two types of elementary particles whose interactions are governed by quantum chromodynamics (QCD), the theory of strong interaction. In the early universe, these particles moved (nearly) freely in a quark-gluon plasma.
This is a joint press release of University Muenster and Heidelberg as well as the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt.
Then, in a phase transition, they combined and formed hadrons, among them the building blocks of atomic nuclei, protons and neutrons. In the current issue of...
Thin-film solar cells made of crystalline silicon are inexpensive and achieve efficiencies of a good 14 percent. However, they could do even better if their shiny surfaces reflected less light. A team led by Prof. Christiane Becker from the Helmholtz-Zentrum Berlin (HZB) has now patented a sophisticated new solution to this problem.
"It is not enough simply to bring more light into the cell," says Christiane Becker. Such surface structures can even ultimately reduce the efficiency by...
A study in the journal Bulletin of Marine Science describes a new, blood-red species of octocoral found in Panama. The species in the genus Thesea was discovered in the threatened low-light reef environment on Hannibal Bank, 60 kilometers off mainland Pacific Panama, by researchers at the Smithsonian Tropical Research Institute in Panama (STRI) and the Centro de Investigación en Ciencias del Mar y Limnología (CIMAR) at the University of Costa Rica.
Scientists established the new species, Thesea dalioi, by comparing its physical traits, such as branch thickness and the bright red colony color, with the...
Scientists have succeeded in observing the first long-distance transfer of information in a magnetic group of materials known as antiferromagnets.
21.09.2018 | Event News
03.09.2018 | Event News
27.08.2018 | Event News
25.09.2018 | Life Sciences
25.09.2018 | Life Sciences
25.09.2018 | Life Sciences