The investigators created a large panel of novel mouse GAT1 transporters tagged with cyan or yellow fluorescent proteins (CFP and YFP) and optimized their expression in neuroblastoma cells. They determined the trafficking, subcellular localization, and oligomerization state of mGAT1 and correlated these features with transporter function.
One finding is that individual components of the FRET amplitude distribution reveal GAT1 dimers, high-order oligomers (likely tetramers), and oligomers associated via PDZ-mediated interactions with the actin cytoskeleton. Secondly, these details of the FRET amplitude distribution correlate with transporter function. Finally, the mGAT1 C-terminus PDZ-interacting domain is necessary for anchoring functional transporters to the actin cytoskeleton at the cell periphery; the corresponding FRET signal appears only in mGAT1 constructs with wild-type function. More generally, the results show the power of the FRET-based approach for distinguishing among distinct states of proteins.
About the Journal of General Physiology
Founded in 1918, the Journal of General Physiology (JGP) is published by the Rockefeller University Press. All editorial decisions on manuscripts submitted are made by active scientists. JGP content is posted to PubMed Central, where it is available to the public for free six months after publication. Authors retain copyright of their published works and third parties may reuse the content for non-commercial purposes under a creative commons license. For more information, please visit www.jgp.org.
Moss, F.J., et al. 2009. J. Gen. Physiol. doi:10.1085/jgp.200910314.
Rita Sullivan | EurekAlert!
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
17.08.2018 | Physics and Astronomy
17.08.2018 | Information Technology
17.08.2018 | Life Sciences