Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Expression and interaction of fluorescently labelled proteins makes living cells glow in different colours

08.03.2007
A novel assay allows simultaneous detection of individual proteins and their interactions in living cells

Protein interactions direct cellular functions and their responses to pathogens and are important therapeutic targets. Scientists from the GSF Research Centre for Environment and Health have recently developed a method enabling simultaneous visualization of individual proteins and their interactions in living cells.

This is achieved by engineering the proteins to constantly emit red or blue fluorescent signals and to produce an additional yellow fluorescent signal upon interaction (see image below). Dr. Ruth Brack-Werner, Director of the GSF Institute of Molecular Virology (IMV) explains the decisive advantage of the new approach: “ In previous assays, signals were generated only by interacting proteins, whereas the individual partners remained undetected. However, the absence of signals could not be used to rule out protein interactions since the absence of one or both interaction partners would have the same effect. To overcome this problem Brack-Werner and her team developed the so-called extended bimolecular fluorescence complementation (exBiFC) which allows simultaneous monitoring of individual proteins and their interactions.

Brack-Werner and her colleagues’ groundbreaking research work focusses on mechanisms that control replication of the human immunodeficiency virus (HIV), which causes AIDS. “HIV replication is based on the interaction of cellular proteins with viral proteins. Interactions involving viral regulatory factors have a direct impact on the amount of virus produced by the HIV host cell”, Brack-Werner explains. “Preventing HIV proteins from interacting with their crucial partners is a promising approach to developing novel therapies.”

... more about:
»Assay »Brack-Werner »HIV »Interaction »REV

Therefore the GSF-scientists developed and validated exBiFC with the HIV Rev protein, which is an accelerator of HIV production. Various assays investigating Rev interactions in artificial settings indicate that the activity of Rev depends on the interaction of Rev molecules with each other and with cellular proteins. The latter include Exportin 1, which transports proteins from the nucleus to the cytoplasm and RISP, a modulator of HIV gene expression discovered by the Brack-Werner team in previous studies.

Brack-Werner and her team demonstrated that exBIFC allows visualization of interactions of Rev with itself and with Exportin1 and RISP in living cells. In addition they were able to compare the strengths of the interactions of Rev with its partners by analysing the intensities of the signals in cell images.

ExBiFC has a wide range of potential appllications and represents an important tool for the elucidation of protein interaction networks and discovery of novel antiviral factors. Thus exBIFC has an enormous potential in the battle against leading global health problems such as infectious diseases and cancers.

Michael van den Heuvel | alfa
Further information:
http://www.gsf.de/neu/Aktuelles/Presse/2007/proteine_en.php

Further reports about: Assay Brack-Werner HIV Interaction REV

More articles from Life Sciences:

nachricht Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover

nachricht First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Color effects from transparent 3D-printed nanostructures

New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference

Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

LaserForum 2018 deals with 3D production of components

17.08.2018 | Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

 
Latest News

Smallest transistor worldwide switches current with a single atom in solid electrolyte

17.08.2018 | Physics and Astronomy

Robots as Tools and Partners in Rehabilitation

17.08.2018 | Information Technology

Climate Impact Research in Hannover: Small Plants against Large Waves

17.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>