Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New hope for regenerative medicine

16.02.2007
Hematopoietic reconstitution with uniparental stem cells

In the February 15th issue of G&D, Dr. K. John McLaughlin and colleagues report on their success in using uniparental embryonic stem cells to replace blood stem cells in mice. Uniparental embryonic stem cells are an appealing alternative source of patient-derived embryonic stem cells, as they have several advantages over embryonic stem cell lines generated by somatic cell nuclear transfer (also known as therapeutic cloning).

Normal mammalian embryos inherit one set of chromosomes from the mother, and one set from the father. Embryos that inherit both sets of chromosomes from the same parent are not viable. They can, however, generate "uniparental" embryonic stem cell lines. Uniparental embryos with two maternal sets of chromosomes are known as parthenogenetic. These embryos have been considered a potential source of embryonic stem cell derived tissues for transplantation into the female from which they were derived.

This study shows for the first time that parthenogenetic blood cells can replace those of an immunocompromised adult mouse. McLaughlin and colleagues also show that this is also possible using embryonic stem cells where both genomes are solely derived from sperm of one male (androgenetic), adding fertile males to the potential patient pool.

Since uniparental ES cells are not derived from viable embryos, their harvesting and use sidesteps many of the ethical concerns that plague traditional ES cell therapies. However, uniparental ES cell research faces the biological hurdle of genomic imprinting, in which specific gene expression patterns are dictated by the parental origin. Uniparental cells only have the imprinting marks for one parental type (or "from one parent") with unknown consequences if harvested and transplanted into adults.

Previous work has shown that uniparental ES cells have only limited ability to contribute to fetal and postnatal development in chimeric animals, with androgenetically-derived uniparental ES cell chimeras displaying abnormal phenotypes and increased lethality. In their current paper, Dr. McLaughlin and colleagues tested the functionality of uniparental ES cells in adult tissues.

"It has been known for over a decade that uniparental cells had some capacity to form tissues in vitro and in vivo but it was questionable if these embryonic stem cells could generate transplantable material that would proliferate and replace tissues in an adult."

The researchers took a two-step approach: First they injected uniparental ES cells into wild-type blastocyts to generate chimeric animals; then they harvested these chimeric fetal livers for transplant into lethally irradiated hosts. The scientists found that uniparental ES cells, regardless of parent-of-origin, were able to functionally reconstitute the entire hematopoietic system of adult mice. Furthermore, the scientists were also able to grow progenitor blood cells in culture from uniparental ES cells, and upon transplant into irradiated adult mice, show that these cells contribute, long-term, to the function of their hematopoietic system.

One issue in using uniparental ES cells for tissue transplants is their durability and safety. McLaughlin's group were able to maintain animals for over 12 months with entirely uniparental blood and were able to rescue irradiated mice with bone marrow transplants from these animals. This unambiguously proves that the transplanted uniparental cells could produce hematopoietic stem cells.

"The ability of the "sperm derived" androgenetic cells to replace adult blood was totally unexpected based on what occurs with these cells during development. The male derived androgenetic cells were at least as effective as the maternal derived cells."

Dr. McLaughlin's new paper expands the horizons for regenerative medicine, not only by demonstrating that uniparental stem cells can form adult-transplantable progenitor cells in cell culture, but also by illustrating the potential utility of androgenetic, as well as parthenogenetic ES cells.

Heather Cosel | EurekAlert!
Further information:
http://www.cshl.edu

More articles from Life Sciences:

nachricht O2 stable hydrogenases for applications
23.07.2018 | Max-Planck-Institut für Chemische Energiekonversion

nachricht Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

Detecting damage in non-magnetic steel with the help of magnetism

23.07.2018 | Materials Sciences

Researchers move closer to completely optical artificial neural network

23.07.2018 | Information Technology

Enabling technology in cell-based therapies: Scale-up, scale-out or program in-place

23.07.2018 | Health and Medicine

VideoLinks
Science & Research
Overview of more VideoLinks >>>