This type, Salmonella serovar Typhimurium DT104, is resistant to at least five different antibiotics. Dutch researcher Armand Hermans found new genetic information in DNA of DT104 that might be involved in its survival and infection mechanism. This genetic information might also be involved in the increase in the number of infections caused by this pathogen.
By comparing the DNA of Salmonella serovar Typhimurium DT104 with the known DNA code of another Salmonella strain, Hermans found new DNA fragments in DT104. These pieces of DNA were found to contain genetic information that might play a role in the survival and infectiousness of this pathogen. The presence of such extra genetic characteristics can make the pathogen stronger and more infectious.
To examine how DT104 behaves to survive various "extreme" conditions, the switching on and off of 500 genetic characteristics was studied. This happened under different conditions such as in a hot, acid or oxygen-free environment. Almost all of the survival characteristics were found to be active under all conditions, whereas the pathogenic characteristics were only active under a few of the conditions. Therefore this pathogen always does everything it can to survive under all conditions, for example, during food conservation or in gastric acid. The pathogenic characteristics of DT104 on the other hand are only active in the intestines where the infection takes place.
Evolution of the pathogen
The DNA of the pathogen says something about how it survives and is transmitted. When a pathogen reproduces, the DNA can change a bit and this can lead to changes in the genetic characteristics. This can, for example, lead to antibiotic resistance but also heat or acid resistance. The pathogens with the best genetic characteristics can spread and survive better and are therefore more infectious: the evolution of a pathogen. Examining which genetic characteristics are present in an infectious Salmonella (in this case the DT104 type) can reveal how this pathogen has become stronger and caused more outbreaks. This information can also be used to make a less dangerous variant of this infectious Salmonella. Such a harmless variant can be used as a vaccine.
Salmonella serovar Typhimurium DT104 is an antibiotic-resistant pathogen that is transmitted via food and is considered to be dangerous for humans. In recent decades the number of infections with this variant has increased in many parts of the world. This research was funded by NWO and contributes to knowledge about the characteristics and behaviour of this dangerous Salmonella.
Dr Armand Hermans | alfa
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
17.08.2018 | Physics and Astronomy
17.08.2018 | Information Technology
17.08.2018 | Life Sciences