Salmonella food poisoning costs the UK economy around £1 billion every year and severe cases can become life threatening for the young and the elderly. The researchers from the Max Planck Institute for Infection Biology and the Institute of Food Research hope that a better understanding of how the pathogen copes with stress will help develop new ways of fighting and preventing infections.
When the bacterium infects its host, it must survive a range of harsh conditions from strong acids in the stomach, to anaerobic and salty environments in the intestine. To adapt to these different conditions, Salmonella must continuously remodel its bacterial “skin” by inserting outer membrane proteins (OMPs) into the cell wall which regulate the transport of salts and allow the bacterium to communicate with its environment. This research, published in the journal Molecular Microbiology, reveals that Salmonella uses a surveillance loop to constantly monitor levels of OMPs to respond fast to signs of stress by switching off protein expression using molecules called small RNAs (sRNAs). These bind to the messenger RNA (mRNA) molecules responsible for the production of OMPs.
“The OMP messenger RNA is unusually stable – it has a half-life of around 15 minutes compared with 5 minutes for other Salmonella mRNAs so it was a mystery how the bacterium could switch off OMP production so quickly” explains Professor Jay Hinton of the Institute of Food Research, “We discovered that a different type of RNA called sRNA binds to the OMP mRNA and blocks its action. The cell then degrades the resulting double stranded RNA molecule. Our novel finding is that these sRNAs called RybB and MicA are able to bind to so many different types of OMP mRNA molecule – most sRNAs bind to only one type.”
The research, supported by the Biotechnology and Biological Sciences Research Council (BBSRC), used a revolutionary new approach combining bioinformatics techniques with cutting edge microarray technology to first identify the sRNAs and then search the 5000 genes in the Salmonella genome for the targets to which they bind. The researchers then confirmed these results by studying a mutant strain lacking the sRNA mechanism.
“We call this a surveillance loop because it allows Salmonella to be constantly vigilant, just like George Orwell’s Big Brother” says Dr Joerg Vogel who led the research at the Max Planck Institute for Infection Biology. “As soon as a problem is detected, the bacterium responds in the same way as the police upon witnessing a crime on CCTV; in this case these small RNAs are deployed, rather than policemen. If Salmonella cannot detect and deal with the problem, however, it becomes extremely stressed.”
Researchers are hoping to apply the same technologies to other pathogenic bacteria. This knowledge could be used in the future to exploit the weaknesses of these dangerous bacteria and to help develop new antibacterials to combat infections when current antibiotics stop working. The proportion of Salmonella bacteria that are resistant to current antibiotics is increasing each year.
This research is particularly relevant as Christmas approaches; whilst only 1 in 20 of turkeys sold for meat in the UK are contaminated with Salmonella, two-thirds of these bacteria are resistant to one or more antibiotics, although researchers stress there is no danger to health if the meat is properly cooked. It is likely that the new surveillance loop discovered here is one of the tools that allow Salmonella to be so successful at infecting both animals and humans.
• Since the beginning of the 1990s, strains of Salmonella enterica sv. Typhimurium resistant to a range of antibiotics have emerged and are threatening to become a serious public health problem, particularly in developing countries.
• Since 1885, a total of 2213 types of salmonella have been identified. They vary in the severity of illness they cause.
• Symptoms of salmonellosis (food poisoning caused by Salmonella) are fever, headache, abdominal pain, diarrhoea, nausea and vomiting, and are usually self-limiting after a week. In some cases, particularly in the young and very elderly, dehydration can become severe and life threatening.
• Salmonella Typhimurium can be found in a broad range of animals, birds and reptiles as well as the environment. It causes food poisoning in humans mainly through the consumption of raw or undercooked contaminated food of animal origin - especially poultry, eggs, meat, salad vegetables and milk.
Zoe Dunford | alfa
Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY
NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation
A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.
The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
20.07.2018 | Power and Electrical Engineering
20.07.2018 | Information Technology
20.07.2018 | Materials Sciences