Scientists also create modified plants to identify and characterize the functions of specific genes. The current issue of Cold Spring Harbor Protocols—released online today (www.cshprotocols.org)—includes a set of techniques for the creation of transgenic plants.
One of the protocols, freely available at http://www.cshprotocols.org/cgi/content/full/2006/30/pdb.prot4668, describes the use of a bacterium, Agrobacterium, to create transgenic Arabidopsis plants. Arabidopsis is used in many studies due to its short reproductive cycle, ease of cultivation, and close relatedness to economically important species such as broccoli and cauliflower. Agrobacterium contains a small chromosome—called the Ti plasmid—into which scientists can insert a gene of interest.
This ‘transgene’ is transferred to Arabidopsis through natural infection with Agrobacterium.
The highlighted article from CSH Protocols describes three techniques that encourage Agrobacterium to infect Arabidopsis plants: dipping an Arabidopsis flower directly into a solution containing Agrobacterium, mechanically forcing the Agrobacterium into the plant cells by applying vacuum, and simply spraying an Agrobacterium suspension onto the plants.
Magic number colloidal clusters
13.12.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
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13.12.2018 | University of Alberta
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A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.
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A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.
Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...
Over the last decade, there has been much excitement about the discovery, recognised by the Nobel Prize in Physics only two years ago, that there are two types...
What if a sensor sensing a thing could be part of the thing itself? Rice University engineers believe they have a two-dimensional solution to do just that.
Rice engineers led by materials scientists Pulickel Ajayan and Jun Lou have developed a method to make atom-flat sensors that seamlessly integrate with devices...
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