The researchers, led by Howard Hughes Medical Institute investigator Angelika Amon, published their findings online May 3, 2006, in the journal Nature. Amon and her colleagues are at the Massachusetts Institute of Technology.
Most cells in the human body – all those other than sperm and eggs – contain 23 pairs of chromosomes. These cells divide through mitosis, a process that creates daughter cells with the same complement of chromosome pairs as the parent. Sperm and egg cells, on the other hand, must contain only half the chromosomes of their parent cells, so that the normal chromosome number will be restored when the sperm and egg unite during fertilization. To achieve this, they are produced through meiosis.
Gluelike protein complexes called cohesins, which hold the members of a chromosome pair together until just the right moment during cell division, are central to both processes. Bound together by cohesins, chromosome pairs must organize themselves in preparation for cell division before they can be released.
According to Amon, a deeper basic knowledge of the mechanism of cohesin loss during meiosis could ultimately improve understanding of the origins of miscarriages and mental retardation due to mis-segregation of chromosomes.
"We first need to understand the key regulatory players and the molecular mechanisms that cause chromosomes to segregate in this very unusual way during meiosis," she said. "Once we have a good enough understanding, then we can ask, for example, what exactly happens to cohesins in older women that make them more likely to give birth to children with an abnormal chromosome number."
According to Amon, knowledge about the mechanism of cohesin function has remained sketchy, even though it plays a central role in meiosis. Researchers knew that an enzyme called separase snips apart cohesins, targeting a specific subunit of the cohesin complex called Rec8. Also, she said, researchers had found that Rec8 cleavage was promoted by phosphorylation -- the addition of chemical phosphate groups -- of Rec8.
Researchers also knew that cohesins release chromosome pairs from one another’s embrace quite differently during meiosis and mitosis. In mitosis, cohesins release chromosomes along their entire length simultaneously. However, in the initial stage of meiosis, cohesins first release only the "arms" of chromosomes, still holding the chromosomes together at their central connection point, the centromere. Only in a second stage of meiosis that gives rise to haploid sperm or egg cells do centromeric cohesins become cleaved. This precisely controlled centromeric "stickiness" is essential for the accurate segregation of sister chromatids into separate cells.
"The key question we wanted to explore was how this step-wise loss of cohesins in meiosis was regulated," said Amon. "It could be that the enzyme separase was the key regulatory player, or it could be that it was the phosphorylation of cohesins that was central."
To find out, the researchers experimented with yeast cells, selectively mutating the Rec8 subunit so that it could not be phosphorylated. They then studied how the cell’s inability to phosphorylate Rec8 affected meiosis. Those experiments showed that phosphorylation is, indeed, important for governing the step-wise loss of cohesins, said Amon. She also noted that research published in two complementary papers from the laboratories of Kim Nasmyth and Yoshinori, which also appeared in Nature, demonstrated that cohesins can be retained at the centromeres during meiosis by enzymes that remove phosphates from the cohesins, counteracting the effects of phosphorylation.
Amon and her colleagues found that phosphorylation was not the only process essential for cohesion removal. Recombination – an exchange of DNA between chromosomes that promotes genetic diversity – was also needed for the initial removal of cohesin from the chromosome arms, they found.
In meiotic recombination, after each member of a chromosome pair has replicated to produce identical sister chromotids in the initial stage of meiosis, the chromosomes exchange arm segments. Only after this exchange, or recombination, do the cells proceed to the second stage of meiosis -- dividing without chromosome replication to produce haploid sperm or egg cells. Recombination is essential for cohesins to get removed from chromosome arms before they are removed from centromeres, Amon said.
"For a long time, people did not think that recombination played any role in establishing the step-wise cohesin loss pattern," she said. "But our experiments showed that recombination is absolutely essential to remove cohesins from chromosome arms during the initial meiotic stages, and if you don’t have recombination that does not happen properly."
Amon also said that the Nature paper represents a significant advance in understanding the enzyme that phosphorylates Rec8, named Polo kinase, because it more accurately identified the specific sites on the Rec8 subunit that Polo phosphorylates. Firm identification of such sites will enable more precise studies of the mechanism of function of Rec8, and will also enable scientists to identify other protein targets of Polo kinase, she said.
Amon emphasized that the discovery of the importance of phosphorylation and recombination is only the beginning of understanding the intricate, critical process of cohesin loss. "There are no doubt other mechanisms at work in cohesin loss, but at this point we don’t know what they are," she said. She theorized, however, that regulation of the cohesin-snipping enzyme separase plays a role in regulating step-wise cohesin loss; she and her colleagues are now exploring that possibility.
Jennifer Michalowski | EurekAlert!
Barium ruthenate: A high-yield, easy-to-handle perovskite catalyst for the oxidation of sulfides
16.07.2018 | Tokyo Institute of Technology
The secret sulfate code that lets the bad Tau in
16.07.2018 | American Society for Biochemistry and Molecular Biology
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
16.07.2018 | Physics and Astronomy
16.07.2018 | Life Sciences
16.07.2018 | Earth Sciences