Spliceosomal fidelity
Pre-messenger RNA (pre-mRNA) splicing is an integral step in gene expression, removing introns that would otherwise disrupt the coding potential of gene transcripts. As published in the August 15th issue of G&D, Drs. Tommaso Villa and Christine Guthrie at UCSF have identified a novel splicing fidelity factor, as well as provide additional evidence for a two-step model of pre-mRNA splicing.
The researchers found that deletion of the yeast gene ISY1 reduces the efficiency of the first step of the splicing reaction and decreases 3-splice site accuracy. Dr. Villa and Guthrie believe that Isy1p interacts with the ATPase Prp16p to regulate spliceosomal conformation, and thereby the fidelity of pre-mRNA splicing.
Dr. Guthrie explains that “15 years ago we hypothesized that the ATPase Prp16 acted as a proofreading clock to limit errors in splicing; the isolation of a Prp16 antagonist (Isy1) provides critical validation for this model and identifies an unexpected fidelity factor.”
Media Contact
More Information:
http://www.cshl.eduAll latest news from the category: Life Sciences and Chemistry
Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.
Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.
Newest articles
Superradiant atoms could push the boundaries of how precisely time can be measured
Superradiant atoms can help us measure time more precisely than ever. In a new study, researchers from the University of Copenhagen present a new method for measuring the time interval,…
Ion thermoelectric conversion devices for near room temperature
The electrode sheet of the thermoelectric device consists of ionic hydrogel, which is sandwiched between the electrodes to form, and the Prussian blue on the electrode undergoes a redox reaction…
Zap Energy achieves 37-million-degree temperatures in a compact device
New publication reports record electron temperatures for a small-scale, sheared-flow-stabilized Z-pinch fusion device. In the nine decades since humans first produced fusion reactions, only a few fusion technologies have demonstrated…