Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Essential genes for embryogenesis uncovered in major mouse mutagenesis project

15.07.2005


A team of scientists led by Cornell University’s Dr. John Schimenti reports today that an extraordinary number of genes are required for prenatal mammalian development. The researchers estimate that up to 19% of all genes are vital for embryogenesis in mice. Their study, which is one of the largest functional genomics projects described to date, is published in today’s online edition of the journal Genome Research.



In addition to the important implications for understanding mammalian developmental biology and the genetic basis for spontaneous abortions, the impressive scale of the study – an enormous logistical effort spanning the past six years – marks a major step forward in the functional annotation of the mouse genome.

"Due to the availability of whole-genome sequences, we are now in the powerful position of knowing the sequence identity of most genes, their locations in the genome, their expression patterns, and which proteins interact with one another," explains Schimenti. "However, identifying the functions of these genes is a much more difficult challenge. For most genes, direct experimentation in the context of a whole organism will be required."


In an effort to explore the functional content of 50 megabases of mouse chromosome 5, or approximately 2% of the genome, Schimenti and his colleagues generated over 1000 mouse pedigrees with random mutations using a chemical called N-ethyl-N-nitrosourea (ENU). They then screened offspring from these pedigrees for lethality and defects in behavior, fertility, fitness, hearing, and vision.

Of 42 mutations identified, an overwhelming majority – 34 mutations – resulted in embryonic lethality. The scientists attributed the lethality to defects in processes including cellular proliferation, gastrulation, cardiovascular function, craniofacial development, neural tube development, and placental development.

Combining their data with estimates from previous studies, the researchers predicted that between 13.7% and 19% of all mouse genes are necessary for embryonic viability. In comparison, research on other organisms has indicated that 6-10% of zebrafish genes and 7% of C. elegans genes are required for prenatal survival. "The higher percentage of embryonic lethal genes in mice may reflect an inherently more complex or sensitive process of development," report the scientists.

The project included major contributions from Schimenti’s collaborators at The University of Pennsylvania School of Medicine (Philadelphia, PA) and The Jackson Laboratory (Bar Harbor, ME), where the study was initiated.

Maria A. Smit | EurekAlert!
Further information:
http://www.cshl.edu

More articles from Life Sciences:

nachricht Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View
22.06.2018 | University of Sussex

nachricht New cellular pathway helps explain how inflammation leads to artery disease
22.06.2018 | Cedars-Sinai Medical Center

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Graphene assembled film shows higher thermal conductivity than graphite film

22.06.2018 | Materials Sciences

Fast rising bedrock below West Antarctica reveals an extremely fluid Earth mantle

22.06.2018 | Earth Sciences

Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View

22.06.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>