Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New Chem-bio Sensors Offer Simultaneous Monitoring

30.06.2005


Researchers at the Georgia Institute of Technology and the Vienna University of Technology have developed a modular system that combines chemical and biological sensing tools capable of providing simultaneous, nano-level resolution information on cell topography and biological activity. The tools integrate micro and nanoscale electrodes into the tips of an atomic force microscope (AFM). A veritable Swiss army knife of sensors, the patented technique is currently being tested to combine other sensing methods to give scientists a more holistic view of cellular activities. The research is published in Vol 44, 2005 of the chemistry journal Angewandte Chemie.



By adding electrodes to the tip of an atomic force microscope, researchers created a tool that can monitor many activities at the same time. "Usually people image topography and then measure the biological activity,” said Christine Kranz, research scientist at Georgia Tech. “But if you think about having biological material, it’s changing with time. So scanning for these sequentially may mean that the structure and activity level of your sample has changed and you’re not looking at the same condition of your sample anymore.”

Using an AFM as the base, researchers added micro and nano-electrodes into the tip. This allows researchers to get biological and chemical information via scanning electrochemical microscopy (SECM) simultaneously with topographical information provided by AFM.


"The problem with conventional AFM imaging is that you get topographical information, but only limited information on the chemical processes occurring at the cell surface,” said Kranz. “With SECM, you can get information on electro-activity, but this information may be convoluted with the topographical information. Also SECM still suffers from limited resolution. We combined the two techniques to give us high resolution topography as well as the chemistry that’s going on at the cell surface.”

Researchers tested their new technique by integrating biosensors for glucose into AFM tips. They imaged, as a synthetic model, glucose transport through track-etched membranes. In another test, a biosensor based on horse radish peroxidase was integrated into the AFM tip. They were able to faithfully measure the chemical activity and image the process to a resolution of 200 nanometers.

The tool promises to be valuable for a wide range of biomedical and biotechnological applications. In an NIH-funded project in collaboration with Emory University, Kranz and Boris Mizaikoff are currently using this technique to study cystic fibrosis and the role errors in regulating adenosine triphosphate (ATP), a chemical involved in transporting energy to cells, might play in the disease.

"The system’s modular design allows it to be adapted for many uses,” said Kranz. Researchers at the Applied Sensors Laboratory at Georgia Tech are testing integrating optical microscopy with the AFM. Another project adds an infrared sensor, while yet another adds a pH sensor. These sensors could also be combined to have three or more sensors on a single instrument because the technology is in the modified AFM tip.

"The technology is very flexible and can be adapted to sense for a wide range of biological systems and processes. Using this technique, we can get a more complete picture of what is going on in a given biological system,” said Kranz.

The research team consisted of Kranz, Mizaikoff, and former postdoc Angelika Kueng from Georgia Tech. Focused ion beam milling was done by Alois Lugstein and Emmerich Bertagnolli from the Vienna University of Technology. Since January 2005, Georgia Tech has established a Focused Ion Beam Center led by Mizaikoff enabling tip fabrication, characterization, and measurements on campus.

David Terraso | EurekAlert!
Further information:
http://www.gatech.edu/news-room/release.php?id=588
http://www.icpa.gatech.edu

More articles from Life Sciences:

nachricht Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY

nachricht NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

A smart safe rechargeable zinc ion battery based on sol-gel transition electrolytes

20.07.2018 | Power and Electrical Engineering

Reversing cause and effect is no trouble for quantum computers

20.07.2018 | Information Technology

Princeton-UPenn research team finds physics treasure hidden in a wallpaper pattern

20.07.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>