Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Study Methods, Strains of Pfiesteria Are Both Critical in Determining Organism’s Toxicity

15.02.2005


Flagellated sexual cell of one of the two known toxic Pfiesteria species, Pfiesteria shumwayae, that were included in the study. Photo by M. Parrow, Center for Applied Aquatic Ecology.


To gauge the toxicity of Pfiesteria, the important single-celled fish predator that was the culprit behind a number of fish kills and fish diseases along the East Coast in the 1990s, researchers need to both use the proper study methods and recognize that certain populations of the organism, called strains, are toxic while others are not.

That’s the main result of a wide-ranging study by Dr. JoAnn M. Burkholder, professor and director of the Center for Applied Aquatic Ecology at North Carolina State University, along with a dozen colleagues from several universities and research institutions, and a federal laboratory specializing in marine toxins. The research is published online in Proceedings of the National Academy of Sciences.

The study reaffirmed that some strains of the two known Pfiesteria species are toxic, including a strain used by other researchers who had asserted that Pfiesteria can’t make toxin. Burkholder and her colleagues found that toxic Pfiesteria strains can produce toxin in the absence of bacteria or other contaminating microbes, and that small amounts of Pfiesteria can kill fish with toxin. They also showed that toxin from Pfiesteria can cause fish disease and death without Pfiesteria cells having to be present: water that at one time contained Pfiesteria, but was then completely filtered to remove all of the toxic Pfiesteria cells, caused lesions in fish.



Other experiments showed that purified Pfiesteria toxin residue added to cultures of larval fish killed the fish, while control fish without exposure to the toxin remained healthy.

In the study, two basic methods of detecting Pfiesteria toxicity were compared: fish microassays (FMAs) using larval fish, and standardized fish bioassays (SFBs) using juvenile fish. In each method, the scientists tested toxic Pfiesteria strains, known to be toxic from toxin detection tests that were completed by the National Oceanic and Atmospheric Administration’s National Ocean Service in Charleston, S.C. The researchers compared the results from the known toxic strains to tests of a strain of Pfiesteria previously reported as nontoxic, and with controls that included both Pfiesteria strains which did not express toxicity and a related nontoxic, single-celled organism similar to Pfiesteria.

FMAs were used to test larval fish in small containers with and without direct exposure to Pfiesteria. These tests examined whether direct contact with Pfiesteria was needed to cause death, and whether fish could become sick or die without direct contact with Pfiesteria.

FMAs had been proposed by other scientists as useful tests for ruling out the presence of toxic Pfiesteria – if fish don’t die when exposed to water filtered from a Pfiesteria culture, they asserted, then toxic Pfiesteria doesn’t exist. But lack of fish death does not necessarily mean Pfiesteria’s toxin isn’t there. “Unless the water was tested to see if Pfiesteria toxin was present, all that can be said from such a study is that there wasn’t enough toxin present to cause the fish to die,” explained Dr. Andrew Gordon, second author of the paper and a scientist at Old Dominion University. “More sensitive tests for the chemical toxin itself would be needed to say for sure whether toxin was or wasn’t present.”

Burkholder offered this analogy: “If a person drinks a glass of water from a well that sometimes contains arsenic, and the person doesn’t suddenly become very sick or die, would it be correct to say that the water didn’t contain any arsenic? Surely not – such a test wouldn’t be sensitive enough to detect levels of arsenic that were too low to cause sudden illness or death, but still were present, and still harmful. A test to detect arsenic would be needed.”

SFBs were better able to reliably detect actively toxic Pfiesteria than FMAs, Burkholder says, since SFBs were not developed to distinguish between toxicity and physical attack as factors in fish death, but instead designed to detect toxic Pfiesteria strains, which are known to routinely prey upon fish. “SFBs detected actively toxic strains. Those strains were verified as toxic by also actually testing for chemical toxin that they produced,” said Dr. Alan J. Lewitus, a scientist at the University of South Carolina, Georgetown and a co-author of the paper. “Toxin detection tests like the procedures developed by the National Ocean Service should be done along with fish assays to determine whether a toxic strain is present.”

The researchers also wanted to test whether Pfiesteria can produce toxin without help from bacteria and – as is known for other toxic algae – whether bacteria enhance Pfiesteria’s toxicity. Burkholder and her colleagues found that some strains of Pfiesteria were able to make toxin on their own, without bacteria. “However, when bacteria were present, Pfiesteria made more toxin,” noted Dr. Harold Marshall, a scientist at Old Dominion University and a co-author of the paper, “and a lot more toxin was produced when the toxic strains were given live fish.”

The study showed that conclusions about whether a species of algae is toxic should be based on tests of many strains, rather than one or a few, using culture conditions that encourage toxin production. “It’s also important to keep in mind that Pfiesteria changes when it’s cultured, after years of separation from its natural habitat,” Burkholder added. “Many toxic strains of Pfiesteria, like other toxic algae, even lose their ability to keep making toxin, so young cultures should be used as much as possible. This study clearly shows that Pfiesteria has benign strains and toxic strains, just like other toxic algae – it is the norm, not the exception. Research designed in recognition of that fact will make real progress in advancing scientific understanding about Pfiesteria.”

Dr. JoAnn M. Burkholder | EurekAlert!
Further information:
http://www.ncsu.edu

More articles from Life Sciences:

nachricht First study on physical properties of giant cancer cells may inform new treatments
14.08.2018 | Brown University

nachricht Tiny Helpers that Clean Cells
14.08.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

Im Focus: Lining up surprising behaviors of superconductor with one of the world's strongest magnets

Scientists have discovered that the electrical resistance of a copper-oxide compound depends on the magnetic field in a very unusual way -- a finding that could help direct the search for materials that can perfectly conduct electricity at room temperatur

What happens when really powerful magnets--capable of producing magnetic fields nearly two million times stronger than Earth's--are applied to materials that...

Im Focus: World record: Fastest 3-D tomographic images at BESSY II

The quality of materials often depends on the manufacturing process. In casting and welding, for example, the rate at which melts solidify and the resulting microstructure of the alloy is important. With metallic foams as well, it depends on exactly how the foaming process takes place. To understand these processes fully requires fast sensing capability. The fastest 3D tomographic images to date have now been achieved at the BESSY II X-ray source operated by the Helmholtz-Zentrum Berlin.

Dr. Francisco Garcia-Moreno and his team have designed a turntable that rotates ultra-stably about its axis at a constant rotational speed. This really depends...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

2018 Work Research Conference

25.07.2018 | Event News

 
Latest News

First study on physical properties of giant cancer cells may inform new treatments

14.08.2018 | Life Sciences

Tiny Helpers that Clean Cells

14.08.2018 | Life Sciences

Algorithm provides early warning system for tracking groundwater contamination

14.08.2018 | Earth Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>