Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Virus known for its photo ops makes its movie screen debut

23.08.2004


High-resolution snapshots of a virus attacking its host – which have culminated in a movie of the process – could reveal secrets of viral infection and improve gene therapy techniques, according to a Purdue University research group.


artist’s conception of the T4 virus


T4 virus



Structural biologists including Michael G. Rossmann have obtained clearer pictures of how the T4 virus, long known to infect E. coli bacteria, alters its shape as it prepares to pierce its host’s cell membrane. The complicated infection process requires a flower-like section of the virus, known as the baseplate, to shape-shift by dramatically changing the configuration of the numerous proteins that form it. The team has taken cryoelectron microscope images of the baseplate from different moments in the process and transformed them into a brief animated movie, helping scientists understand how infection occurs and possibly enabling them to apply this knowledge for the benefit of human patients in the future.

"Instead of a still photo of the baseplate, we now have a movie of it opening," said Rossmann, who is Henley Distinguished Professor of Biological Sciences in Purdue’s School of Science. "A better understanding of the infection process is a step forward for fundamental science, but it also could allow scientists to alter the baseplate so that the virus could infect cells other than E. coli. T4 might then be used to deliver beneficial genes to damaged or infected human tissue."


The research was performed at Purdue and the Institute of Bioorganic Chemistry in Moscow by a team of scientists including first author Petr G. Leiman, Paul R. Chipman, Victor A. Kostyuchenko, Vadim V. Mesyanzhinov and Rossmann. The paper appears in the current (Aug. 20) issue of the scientific journal Cell, and it builds on research the team published last year regarding the baseplate of the T4 virus. This previous paper offered a close-up picture of the baseplate at a single moment in time, information that was valuable because of the detail it provided of the part of the virus that attached itself to E. coli’s surface.

"It was good to see the baseplate at such unprecedented resolution, but the infection process is not a still picture – it’s a story," Rossmann said. "We knew we needed to see more than one scene in that story if we were ever to understand its full meaning."

The baseplate is composed of 16 types of protein molecules, most present in multiple copies. Before infection, these proteins fit together to form a hexagonal shape. Together with the 12 legs that extend from the T4’s tail to grasp the victim E. coli, the virus resembles an Apollo moon lander. When the T4 approaches "touchdown" on an E. coli’s cell membrane, the baseplate’s proteins unfold in a complex motion, opening like a flower’s petals and changing shape from a hexagon to a star.

"We can now visualize how these proteins move together, which means a great deal for anyone trying to comprehend infection," Rossmann said. "If you saw a car speed past you for the first time ever, it might impress you, but you probably wouldn’t have much of an idea how it works. But if you stop it, you can examine the engine and find out. That’s essentially what we’ve done – stop the virus at two points in its attack on E. coli and examine the difference."

Scientists speculate that viruses are a key player in the evolutionary process on planet Earth. Far from being mere purveyors of disease, the viral infection process also could be partly responsible for spreading new genes among organisms rapidly and preparing their hosts for future environmental changes. This is part of viruses’ fascination for scientists like those on Rossmann’s team and why some medical professionals seek to use altered viruses to cure illnesses rather than cause them.

"Viruses’ great talent – injecting genetic material into living cells – could make them valuable for delivering healthy DNA to cells damaged by injury or cancer," said Leiman, a postdoctoral researcher in Rossmann’s lab. "T4’s baseplate proteins could be altered so it could infect human cells instead of E. coli. This study could bring us one step closer to using it as a gene therapy vehicle."

Gene therapy using T4 remains a distant possibility, however, and Rossmann said the true value of the team’s latest research was for the fundamental understanding it provides of the viral world.

"Viruses are among the tiniest of biological entities, yet nature has designed them to perform very complicated tasks," he said. "Understanding their behavior will open doors for scientists in many disciplines, especially with biologists, chemists and physicists increasingly working side by side."

As a step in that direction, Rossmann said he hopes that he and his colleagues will be able to obtain a better picture of the components within the tiny mechanism that is the baseplate.

"Our knowledge of the orientation of the proteins within the baseplate could still stand some improvement," he said. "We’d like to look under the hood of this car, so to speak, and determine precisely how the carburetor sits on the engine. Determining the structure and interactions of these proteins will help us down that road."

The research was funded in part by grants from the National Science Foundation, the International Human Frontier Science Program and the Howard Hughes Medical Institute.

Rossmann’s team is associated with Purdue’s Markey Center for Structural Biology, which consists of laboratories that use a combination of cryoelectron microscopy, crystallography, and molecular biology to elucidate the processes of viral entry, replication and pathogenesis.

Michael Rossmann | EurekAlert!
Further information:
http://www.purdue.edu

More articles from Life Sciences:

nachricht Staying in Shape
16.08.2018 | Max-Planck-Institut für molekulare Zellbiologie und Genetik

nachricht Chips, light and coding moves the front line in beating bacteria
16.08.2018 | Okinawa Institute of Science and Technology (OIST) Graduate University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

Im Focus: Lining up surprising behaviors of superconductor with one of the world's strongest magnets

Scientists have discovered that the electrical resistance of a copper-oxide compound depends on the magnetic field in a very unusual way -- a finding that could help direct the search for materials that can perfectly conduct electricity at room temperatur

What happens when really powerful magnets--capable of producing magnetic fields nearly two million times stronger than Earth's--are applied to materials that...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

2018 Work Research Conference

25.07.2018 | Event News

 
Latest News

Staying in Shape

16.08.2018 | Life Sciences

Diving robots find Antarctic seas exhale surprising amounts of carbon dioxide in winter

16.08.2018 | Earth Sciences

Protein droplets keep neurons at the ready and immune system in balance

16.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>