Jefferson Researchers Discover Novel Gene Profile That May Identify Colon Stem Cells

The findings could potentially lead to a better understanding of the role of stem cells in the development of colon cancer

Researchers at Jefferson Medical College have uncovered a novel pattern of gene expression in the stem cell-rich bottom of tiny “crypts” in the tissue lining the colon. By identifying these patterns, the scientists hope to be able to identify mechanisms through which stem cells contribute to the development of colon cancer.

“Having a genetic signature for the colonic stem cell will give us a tool to investigate the hypothesis that stem cell overpopulation is the key to colon cancer initiation,” says Bruce Boman, M.D., Ph.D., director of the Division of Genetic and Preventive Medicine at Jefferson Medical College of Thomas Jefferson University in Philadelphia and Jefferson’s Kimmel Cancer Center, who led the work.

Dr. Boman presents his team’s findings March 30 at the annual meeting of the American Association for Cancer Research in Orlando.

According to Dr. Boman, a form of inherited colon cancer, familial adenomatous polyposis, or FAP, may begin when processes that regulate adult stem cells in the colon go awry. To try to understand some of these processes, Dr. Boman and his team decided to first look at the genes expressed in normal colon crypts. The researchers examined sections of the top, middle and bottom of crypts, using microarray technology to analyze which genes are selectively expressed in each region. They were particularly interested in the genes expressed in the bottom of the crypt, where the stem cells reside.

“We’ve found some intriguing patterns of gene expression – patterns that suggest a unique genetic signature for stem cells,” says research technician Moreh Salunek. “We were looking at genes that were unique and up-regulated in the bottom of the crypt, and found that the majority were related to binding processes and catalytic enzymes.” Some were surprising, she says, such as STAMP1, a gene implicated as a marker for the progression of prostate cancer. They also found a gene called HOXD4, which is a developmental gene involved in the formation of the gut.

Stem cells in the normal colon produce daughter cells that proliferate and make their way to the crypt top, where they differentiate into specialized colon cells, says Dr. Boman. Colon cancer is marked by a change in the distribution pattern of proliferating daughter cells.

Next, the researchers plan to use their novel gene profile to investigate cellular changes in colon cancer development. They also plan to use a different microarray to expand the number of genes they can examine.

Media Contact

Steven Benowitz TJUH

All latest news from the category: Life Sciences and Chemistry

Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Back to home

Comments (0)

Write a comment

Newest articles

Lighting up the future

New multidisciplinary research from the University of St Andrews could lead to more efficient televisions, computer screens and lighting. Researchers at the Organic Semiconductor Centre in the School of Physics and…

Researchers crack sugarcane’s complex genetic code

Sweet success: Scientists created a highly accurate reference genome for one of the most important modern crops and found a rare example of how genes confer disease resistance in plants….

Evolution of the most powerful ocean current on Earth

The Antarctic Circumpolar Current plays an important part in global overturning circulation, the exchange of heat and CO2 between the ocean and atmosphere, and the stability of Antarctica’s ice sheets….

Partners & Sponsors