Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technique for DNA nanostructures

18.09.2002


DNA nanostructures
© University of California - Davis


A new method to make very small patterns of DNA molecules on surfaces has been developed by chemists at the University of California, Davis, and Wayne State University, Detroit. The technique could allow faster and more powerful devices for DNA sequencing, biological sensors and disease diagnosis.

The technique, called nanografting, can be used to make patterns of DNA that are up to a thousand times smaller than those in commercially available microarrays, said UC Davis chemist Gang-yu Liu. Liu developed the method with Christine Chow at Wayne State University and UC Davis graduate students Maozi Liu and Nabil Amro.

"We believe these are the smallest nanostructures of DNA yet made," Liu said. They drew lines as small as 15 nanometers across by 150 nanometers long -- equivalent to eight DNA molecules across. The same method can be applied to make structures as small as two by four nanometers, or a few billionths of an inch, in size.



Microarrays -- arrangements of small dots of DNA or other molecules on slides or chips -- have become a powerful tool in biomedical research. For example, a DNA array can be used to look at thousands of genes in a cell at the same time and see which are switched on or off.

The patterns are made by coating a gold film with long, closely-packed molecules called thiols. The thiols attach to the gold through a sulfur atom at one end and stand upright. The researchers use an atomic force microscope probe -- essentially a very fine needle -- to scrape away some of the thiols. They add short pieces of DNA, called oligonucleotides, which have thiols attached at one end. The thiols stick the DNA onto the exposed gold head-first, leaving the rest of the DNA standing up above the thiol layer.

The researchers showed that the DNA molecules were accessible and chemically active. The research is published in the August 2002 issue of the journal Nano Letters

Andy Fell | EurekAlert!
Further information:
http://www.ucdavis.edu/

More articles from Life Sciences:

nachricht Developing a digital holography-based multimodal imaging system to visualize living cells
02.06.2020 | Kobe University

nachricht Possible physical trace of short-term memory found
02.06.2020 | Institute of Science and Technology Austria

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New measurement exacerbates old problem

Two prominent X-ray emission lines of highly charged iron have puzzled astrophysicists for decades: their measured and calculated brightness ratios always disagree. This hinders good determinations of plasma temperatures and densities. New, careful high-precision measurements, together with top-level calculations now exclude all hitherto proposed explanations for this discrepancy, and thus deepen the problem.

Hot astrophysical plasmas fill the intergalactic space, and brightly shine in stellar coronae, active galactic nuclei, and supernova remnants. They contain...

Im Focus: Biotechnology: Triggered by light, a novel way to switch on an enzyme

In living cells, enzymes drive biochemical metabolic processes enabling reactions to take place efficiently. It is this very ability which allows them to be used as catalysts in biotechnology, for example to create chemical products such as pharmaceutics. Researchers now identified an enzyme that, when illuminated with blue light, becomes catalytically active and initiates a reaction that was previously unknown in enzymatics. The study was published in "Nature Communications".

Enzymes: they are the central drivers for biochemical metabolic processes in every living cell, enabling reactions to take place efficiently. It is this very...

Im Focus: New double-contrast technique picks up small tumors on MRI

Early detection of tumors is extremely important in treating cancer. A new technique developed by researchers at the University of California, Davis offers a significant advance in using magnetic resonance imaging to pick out even very small tumors from normal tissue. The work is published May 25 in the journal Nature Nanotechnology.

researchers at the University of California, Davis offers a significant advance in using magnetic resonance imaging to pick out even very small tumors from...

Im Focus: I-call - When microimplants communicate with each other / Innovation driver digitization - "Smart Health“

Microelectronics as a key technology enables numerous innovations in the field of intelligent medical technology. The Fraunhofer Institute for Biomedical Engineering IBMT coordinates the BMBF cooperative project "I-call" realizing the first electronic system for ultrasound-based, safe and interference-resistant data transmission between implants in the human body.

When microelectronic systems are used for medical applications, they have to meet high requirements in terms of biocompatibility, reliability, energy...

Im Focus: When predictions of theoretical chemists become reality

Thomas Heine, Professor of Theoretical Chemistry at TU Dresden, together with his team, first predicted a topological 2D polymer in 2019. Only one year later, an international team led by Italian researchers was able to synthesize these materials and experimentally prove their topological properties. For the renowned journal Nature Materials, this was the occasion to invite Thomas Heine to a News and Views article, which was published this week. Under the title "Making 2D Topological Polymers a reality" Prof. Heine describes how his theory became a reality.

Ultrathin materials are extremely interesting as building blocks for next generation nano electronic devices, as it is much easier to make circuits and other...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Dresden Nexus Conference 2020: Same Time, Virtual Format, Registration Opened

19.05.2020 | Event News

Aachen Machine Tool Colloquium AWK'21 will take place on June 10 and 11, 2021

07.04.2020 | Event News

International Coral Reef Symposium in Bremen Postponed by a Year

06.04.2020 | Event News

 
Latest News

Perfect optics through light scattering

02.06.2020 | Power and Electrical Engineering

The digital construction site: A smarter way of building with mobile robots

02.06.2020 | Architecture and Construction

Process behind the organ-specific elimination of chromosomes in plants unveiled

02.06.2020 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>