Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

PNNL gathers most complete protein map of "world’s toughest bacterium"

22.08.2002


Scientists at the Department of Energy’s Pacific Northwest National Laboratory have obtained the most complete protein coverage of any organism to date with the study of a radiation-resistant microbe known to survive extreme environments. This research potentially could open up new opportunities to harness this microorganism, called Deinococcus radiodurans, for bioremediation.



A study published in the Aug. 20 issue of the Proceedings of the National Academy of Sciences observed a 61 percent coverage of the microbe’s possible predicted set of proteins, or its proteome. This is the most complete proteome reporting to date of any organism. (The proteome is the collection of proteins expressed by a cell under a specific set of conditions at a specific time.) PNNL scientists identified more than 1,900 proteins in D. radiodurans.

Studying the amount of each protein present at any time has become more important as scientists attempt to learn which proteins are involved in important cellular functions. DOE’s Microbial Genome Program, an element of the Genomes to Life Program, provided the genomic information for various microorganisms, including D. radiodurans, and developed ways to predict the set of possible proteins, which hold the key to why and how these microbes carry out different functions.


D. radiodurans is of interest because of its potential to degrade radioactive materials, its ability to withstand high levels of radiation and its impressive DNA repair capabilities. The Guinness Book of World Records once called it the world’s toughest bacterium.

"We’ve been able to see more of the proteins, especially those proteins that exist in small quantities," said Mary Lipton, PNNL senior research scientist and lead author of the PNAS paper. "Because our coverage is unprecedented, we’re now able to provide biologists with protein-level information they never had access to before."

To identify proteins involved in various functions, PNNL researchers exposed D. radiodurans to several stresses and environments: heat shock; cold shock; exposure to chemicals that damage DNA such as trichloroethylene; exposure to ionizing radiation; and starvation. They were able to identify many proteins previously only hypothesized to exist on the basis of DNA information and also proteins that seemed to have little function. New proteins that became active only during a specific condition also were identified, as were proteins that appeared to exist all the time.

To achieve this unprecedented coverage, researchers used a new high-throughput mass spectrometer based on Fourier-transform ion cyclotron resonance developed at PNNL. This instrumentation allows scientists to identify thousands of proteins within hours. The system relies on a two-step process that first uses tandem mass spectrometry to identify biomarkers for each protein.

"We’ve not only identified the proteins, we have validated our results by using two mass spectrometry techniques," said Richard D. Smith, PNNL principal investigator.

"Once we’ve identified the protein biomarkers, then we never have to repeat the identification step, thereby speeding up our experiments. As a result we not only have a much more complete view of the proteome than existed previously, but we also can follow changes to it much faster."

The experiments were conducted in the William R. Wiley Environmental Molecular Sciences Laboratory, a DOE scientific user facility supported by the Office of Biological and Environmental Research and located at PNNL.

Other authors involved in the research came from Louisiana State University and the Uniformed Services University of the Health Sciences in Bethesda, Md.

Business inquiries on PNNL research and technologies should be directed to 1-888-375-PNNL or e-mail: inquiry@pnl.gov.

Pacific Northwest National Laboratory is a DOE research facility and delivers breakthrough science and technology in the areas of environment, energy, health, fundamental sciences and national security. Battelle, based in Columbus, Ohio, has operated the laboratory for DOE since 1965.

Staci Maloof | EurekAlert!

More articles from Life Sciences:

nachricht Nonstop Tranport of Cargo in Nanomachines
20.11.2018 | Max-Planck-Institut für molekulare Zellbiologie und Genetik

nachricht Researchers find social cultures in chimpanzees
20.11.2018 | Universität Leipzig

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Nonstop Tranport of Cargo in Nanomachines

Max Planck researchers revel the nano-structure of molecular trains and the reason for smooth transport in cellular antennas.

Moving around, sensing the extracellular environment, and signaling to other cells are important for a cell to function properly. Responsible for those tasks...

Im Focus: UNH scientists help provide first-ever views of elusive energy explosion

Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.

Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Optical Coherence Tomography: German-Japanese Research Alliance hosted Medical Imaging Conference

19.11.2018 | Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

 
Latest News

Nonstop Tranport of Cargo in Nanomachines

20.11.2018 | Life Sciences

Researchers find social cultures in chimpanzees

20.11.2018 | Life Sciences

When AI and optoelectronics meet: Researchers take control of light properties

20.11.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>