The ambition in many quarters to scale up the production of human embryonic stem cells and move towards clinical trials requires that different laboratories are able to produce to a standard quality of cells. Developing common standards in stem cell production is not straightforward as so much is still unknown in this new science. Professor Andrew Webster and Dr Lena Eriksson of York University interviewed and observed a range of scientists and technicians working in stem cell laboratories in the UK, USA and Sweden.
Accurately describing human embryonic stem cell lines is one way to begin setting standards. A stem cell line is a family of constantly-dividing cells, the product of a single parent group of stem cells. Embryonic stem cells are unique in that they have yet to 'decide' which developmental path to choose: they have the ability to turn into almost all human cell types. However each human embryonic stem cell holds the genetic signature of the donor which differs between donors just as people themselves differ. Further the state of a stem cell is by its very nature temporary as it is defined by its ability to develop into many different cell types.
Some scientists argued that as the stem cell cannot be standardised, the process and materials used should be standardised. Currently differences in laboratory practices are thought to result in differences in stem cell lines reflecting the way they are treated rather than an innate quality of the lines themselves. The skills of the laboratory technician also play a key role. But pinpointing all the factors that contribute to producing successful stem cell lines remains elusive. “Scientists often explain that their laboratory produces successful human embryonic stem cell lines because their laboratory uses the factor X when they grow them or its lab technicians have green fingers,” says Dr Lena Eriksson of the research team.
Some researchers prefer not to develop standards as these will constrain the science and may close off promising areas of research. “Others argue that it is simply futile,” explains Lena Eriksson. “Can you standardise how all children sleep by giving them the same bed, sheet and blanket? Of course not. So why bother standardising the materials of stem cell production when other differences such as donor history and derivation methods are so complex, manifold and, to date, largely unknown?”
However, the research shows most stem cell scientists are keen to collaborate on the technical side as they feel this is necessary in order to move the field as a whole forward. The research team followed one particularly successful effort – the International Stem Cell Initiative – that adopted a multi-sited experimental approach in which a large number of stem cell lines were analysed and compared.
Because of the imprecise nature of the manual laboratory work, standardisation opens a potential market for automation technologies to be introduced into human embryonic stem cell laboratories. Yet the research shows this also brings tensions. By attracting businesses keen to become suppliers of laboratory material for this emerging market, the expense of such equipment as well as the skills and staff needed to operate it may exclude small laboratories. Even those that can afford to meet the costs have reservations about the robustness of stem cells to withstand the automation process. The relationship between standards, automation and stem cell quality will be key to the future scale-up of the field and so its clinical application.
Danielle Moore | alfa
Lethal combination: Drug cocktail turns off the juice to cancer cells
12.12.2018 | Universität Basel
Smelling the forest – not the trees
12.12.2018 | Universität Konstanz
A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.
The widely used anti-diabetes drug metformin not only reduces blood sugar but also has an anti-cancer effect. However, the metformin dose commonly used in the...
A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.
Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...
Over the last decade, there has been much excitement about the discovery, recognised by the Nobel Prize in Physics only two years ago, that there are two types...
What if a sensor sensing a thing could be part of the thing itself? Rice University engineers believe they have a two-dimensional solution to do just that.
Rice engineers led by materials scientists Pulickel Ajayan and Jun Lou have developed a method to make atom-flat sensors that seamlessly integrate with devices...
Scientists at the University of Stuttgart and the Karlsruhe Institute of Technology (KIT) succeed in important further development on the way to quantum Computers.
Quantum computers one day should be able to solve certain computing problems much faster than a classical computer. One of the most promising approaches is...
12.12.2018 | Event News
10.12.2018 | Event News
06.12.2018 | Event News
12.12.2018 | Health and Medicine
12.12.2018 | Physics and Astronomy
12.12.2018 | Health and Medicine