Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Gene-expression profiling of the effects of liver toxins

20.06.2008
Gene-expression data from liver tissue or whole blood can be used to classify histopathologic differences in the effects of hepatotoxins.

It is hoped that these findings, published in BioMed Central’s open access journal, Genome Biology, will lead to a more precise way of defining the potential hepatotoxicity of new compounds.

It is already known that toxins can be classified using transcriptomic data taken from the primary target tissue or organ. In this new work, researchers set out to see if expression data from blood could serve as a surrogate for a target organ.

A team from the National Institute of Environmental Health Sciences (NIEHS), part of the National Institutes of Health, and Cogenics, a division of Clinical Data, Inc., produced an extensive set of gene expression data combined with more traditional toxicological measurements, such as clinical chemistry and histopathology, after exposing rats to different known hepatotoxic compounds.

... more about:
»blood »liver »toxins

Rodents were treated with one of the eight hepatotoxicants being studied at varying doses designed to induce liver injury (either moderate, severe, or no measurable injury), or with a vehicle control. Data relating to histopathology, clinical chemistry, hematology and gene expression were collected from whole blood and from the liver at different timepoints following exposure.

The researchers confirmed that gene expression data from the target organ can be used to classify and differentiate toxins, and went on to show that classification is also possible using data from whole blood. One of the study’s co-authors, Edward K. Lobenhofer says: “These data illustrate the power of gene expression profiling to resolve differences in the physical manifestation of the injury evoked by different toxicants using samples derived from either target tissue or whole blood. Additionally, this study demonstrates the possibility of classifying differences in these types of injury using data generated from blood samples.”

The results emphasise the importance of ‘phenotypic anchoring’ – linking gene expression changes to traditional measures of toxicology. “Our results powerfully underscore the importance of anchoring gene expression data analysis through consistent phenotypic endpoints” says co-author Raymond W. Tennant, Head of the Cancer Biology Group at the NIEHS. “Through phenotypic anchoring we are able to facilitate the identification of genes useful in compound classification.”

The comprehensive dataset from the study has been made freely available through a publicly accessible website (http://cebs.niehs.nih.gov) and will be a valuable resource for the systems toxicology research community.

Charlotte Webber | alfa
Further information:
http://genomebiology.com/
http://www.biomedcentral.com/

Further reports about: blood liver toxins

More articles from Life Sciences:

nachricht Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg

nachricht Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: UNH scientists help provide first-ever views of elusive energy explosion

Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.

Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Purdue cancer identity technology makes it easier to find a tumor's 'address'

16.11.2018 | Health and Medicine

Good preparation is half the digestion

16.11.2018 | Life Sciences

Microscope measures muscle weakness

16.11.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>