Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Protein deficiency leads to faster fat burning in mice

14.03.2008
Researchers have developed a new, lean mouse with characteristics suggesting that someday, using medication to manipulate a specific protein in humans could emerge as a strategy to treat obesity and disorders associated with excess weight, such as diabetes and metabolic syndrome.

To create the hybrid, scientists crossed mice deficient in protein kinase C beta (PKCB) with the C57 black mouse, a common animal used in research for studying diabetes and obesity.

“These animals can eat more than normal. And they have less fat than normal. That’s a dream come true if it can be extended to human beings,” said Kamal Mehta, senior author of the study and a professor of molecular and cellular biochemistry at Ohio State University.

He noted, however, that an appropriate therapy for humans would take years to develop.

At first glance, compared to mice with no deficiency, these new hybrid mice were smaller and leaner. And when the researchers looked under their skin, they saw the mice had less fat distribution in the skin itself and less fat tissue overall. They also had less fat in their livers and muscles. The fat cells they did have were smaller than fat cells in other mice.

And despite the propensity for obesity from their original genes, the new mice lost weight while eating up to 30 percent more food than other mice. This means their lower weight was not caused by less eating, suggesting the protein deficiency corrected for the obesity tendencies by increasing the hybrids’ ability to burn fat, said Mehta, an investigator in Ohio State’s Davis Heart and Lung Research Institute.

The research is published in a recent issue of the Journal of Biological Chemistry.

Based on his previous research on the role of PKCB in metabolism, Mehta expected a deficiency of the protein to affect how the body processes triglycerides, or fat stored in body tissue.

“The bottom line is we were the first to show that this deficiency leads to a lean animal. The next question is why,” Mehta said. “In order to answer why, we need to know which genes are changed in these knockout animals.”

The most prominent effect the scientists have been able to identify so far relates to the mitochondria, the principal energy source of cells. Mehta said the new hybrid mice have more mitochondria within their cells than do normal mice, and that the added energy source allows them to convert fatty acids into energy.

“We have shown to some extent that there is increased fatty acid oxidation. We found that they use more oxygen, so that means they are using this oxygen to metabolize fat, convert it into carbon dioxide and expel it when they breathe,” said Madhu Mehta, a clinical consultant and co-author on the study and assistant professor of internal medicine at Ohio State.

The research group is testing this finding with an additional experiment, introducing the PKCB deficiency to animals with a lower production of mitochondria to see if the level of mitochondria increases when the protein is not present.

More work also needs to be done to determine whether the protein could be deficient in just certain types of cells to produce the same effect – for example, by eliminating the protein from only liver cells or fat tissue cells rather than throughout the body. Under current circumstances, the deficiency is present in the entire mouse genome.

“So we need to find which specific tissue needs the deficiency. Once we know which tissue is crucial for this, we can target that,” Kamal Mehta said. “The whole idea is to be able to develop a drug that would safely create this deficiency in humans.”

Mehta also is leading a study testing the effect of PKCB deficiency on diabetes in particular, examining whether the disease can be prevented by the elimination of this protein. An excess of triglycerides in tissue can lead to insulin resistance, a hallmark of diabetes. Because the protein relates to how the body burns triglycerides, Mehta believes the deficiency also could play a role in preventing the disease from developing.

The deficiency does not appear to pose any health problems. The mice with the deficiency lived a normal lifespan and experienced no premature deaths.

It remains unknown whether the deficiency currently exists naturally in humans. “Genetic testing of lean people could help answer that question,” Mehta said.

This research was supported by the National Institutes of Health.

Co-authors on the study were Rishipal Bansode, Wei Huang and Sanjit Roy of the Department of Molecular and Cellular Biochemistry and the Davis Heart and Lung Research Institute.

Kamal Mehta | EurekAlert!
Further information:
http://www.osu.edu

Further reports about: Diabetes HDL-cholesterol Mehta PKCB Tissue deficiency mitochondria

More articles from Life Sciences:

nachricht Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover

nachricht First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Color effects from transparent 3D-printed nanostructures

New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference

Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...

Im Focus: Unraveling the nature of 'whistlers' from space in the lab

A new study sheds light on how ultralow frequency radio waves and plasmas interact

Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...

Im Focus: New interactive machine learning tool makes car designs more aerodynamic

Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.

When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...

Im Focus: Robots as 'pump attendants': TU Graz develops robot-controlled rapid charging system for e-vehicles

Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.

Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....

Im Focus: The “TRiC” to folding actin

Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.

Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

LaserForum 2018 deals with 3D production of components

17.08.2018 | Event News

Within reach of the Universe

08.08.2018 | Event News

A journey through the history of microscopy – new exhibition opens at the MDC

27.07.2018 | Event News

 
Latest News

Smallest transistor worldwide switches current with a single atom in solid electrolyte

17.08.2018 | Physics and Astronomy

Robots as Tools and Partners in Rehabilitation

17.08.2018 | Information Technology

Climate Impact Research in Hannover: Small Plants against Large Waves

17.08.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>