Potential pharmaceutical compounds which only present a genotoxicity hazard after metabolism, usually by the liver, are known as pro-genotoxins. The utility of GreenScreen HC has now been extended to detect pro-genotoxins as well as all common mechanistic classes of genotoxin including mutagens, clastogens, aneugens, as well as both topoisomerase and polymerase inhibitors.
Using a standardized 96-well microplate format, the test compound and patented Green Fluorescent Protein (GFP) reporter cells are combined with 1% S9 for a 3 hour exposure period. After exposure, cells are washed to remove the S9 and test compound and then allowed a further 45 hour recovery incubation. Cell viability and GFP fluorescence data are assessed using flow cytometry and simple software generates quantitative results and a graphical output.
Minimal investment in early genotoxicity screening delivers major time savings, avoids unnecessary animal testing and prevents costly late stage failures of lead drug compounds through early detection of genotoxic potential. Combining exceptional levels of specificity and sensitivity, GreenScreen HC also correctly identifies non-carcinogenic compounds known to give misleading positive results in other in vitro genotoxicity assays.
Steve Beasley | alfa
New findings help to better calculate the oceans’ contribution to climate regulation
14.11.2018 | Jacobs University Bremen gGmbH
How algae and carbon fibers could sustainably reduce the athmospheric carbon dioxide concentration
14.11.2018 | Technische Universität München
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
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Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
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On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly
The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...
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14.11.2018 | Life Sciences