Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Relocation of Proteins with a New Nanobody Tool

11.04.2017

Researchers at the Biozentrum of the University of Basel have developed a new method by which proteins can be transported to a new location in a cell. The novel tool enables scientists to study the function of proteins depending on their position by using nanobodies. The tool can be used for a wide range of proteins and in various areas of developmental biology. The scientific journal eLife has published the results.

The research group of Markus Affolter is investigating the growth of the wings of the fruit fly Drosophila to understand which processes control organ development and growth. Proteins that control such growth processes are the focus of their investigations.


Nanobodies (pink) in the wing precursor of a fruit fly larva.

University of Basel, Biozentrum

In this context, not only the composition of the proteins is important, but also their position which can influence protein function. The new nanobody tool of the Affolter research team allows the relocation of proteins and thus to study their function in a position-dependent manner.

Novel tool for all GFP-bound proteins

A repositioning of the proteins of interest requires a labeling with the green fluorescent protein (GFP). Subsequently, so-called anti-GFP nanobodies, small antibody fragments derived from camels, are then used to bind and to move the GFP-tagged proteins to a new site in the living organism. The nanobody itself is linked to a signal protein that defines the destination of the target protein.

Thus, the nanobody forces the GFP-tagged protein into a new position. “Even if we do not know exactly the composition and structure of a protein, we can label it with GFP and control the destination site by using nanobodies,” says Stefan Harmansa, one of the two first authors.

Artificial relocation with nanobodies

The researchers were able to transfer proteins to a new site, internal or external to the cell. “By transporting proteins to new locations, we can observe whether their function changes or not and whether development is affected,” says Ilaria Alborelli, also one of the first authors of the study.

So far, scientists have been restricted in relocating proteins. The new nanobody tool, however, makes it possible to easily and efficiently change the position of all GFP-tagged proteins and thus explore their functions. The Affolter group has already been successful in investigating the growth of Drosophila wings using this nanobody tool. By interfering with the signaling molecule Dpp in a position-dependent manner, the scientists have been able to show more precisely its influence on wing growth.

In the future, the new nanobody tool can be used for a wide variety of studies on organ growth and in various other areas of developmental biology. With this concept, the growth and the development of different cells and organs can be investigated in more detail.

The Affolter team also faces many new challenges. “We as developmental biologists are still confronted with urgent questions such as how an organism knows when it has to stop its growth. To put it succinctly, how does it work that arms or legs stop growing when they reach their correct length?”, says Stefan Harmansa. In the future, the novel tool may contribute to a better understanding of how organ growth is regulated.

Original source

Stefan Harmansa, Ilaria Alborelli, Dimitri Bieli, Emmanuel Caussinus and Markus Affolter
A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila
eLife (2017), doi: 10.7554/eLife.22549

Further information

Prof. Dr. Markus Affolter, University of Basel, Biozentrum, tel. +41 61 207 20 72, email: markus.affolter@unibas.ch
Heike Sacher, University of Basel, Biozentrum, Communications, tel. +41 61 207 14 49, email: heike.sacher@unibas.ch

Heike Sacher | Universität Basel
Further information:
http://www.unibas.ch

More articles from Life Sciences:

nachricht Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY

nachricht NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

A smart safe rechargeable zinc ion battery based on sol-gel transition electrolytes

20.07.2018 | Power and Electrical Engineering

Reversing cause and effect is no trouble for quantum computers

20.07.2018 | Information Technology

Princeton-UPenn research team finds physics treasure hidden in a wallpaper pattern

20.07.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>