Researchers at Umeå University, Sweden, in collaboration with an international team, have discovered a new mechanism for interaction between two proteins that are vital for the Yersinia pseudotuberculosis bacteria’s pathogenic ability.
A common strategy bacteria have to cause disease is to transfer toxic proteins to host cells, for example in humans. Yersinia pseudotuberculosis is a bacteria that can cause gastrointestinal infections. The bacteria transfers proteins called Yop (Yersinia outer protein) through a complex needle structure forming a pore in the host cell membrane.
Yop proteins are made up of segments with different functions. The YopH protein has a segment which counteracts the immun system of the host cells. Another segment binds to chaperones, a group of proteins that help other proteins uphold a correct structure, which is important for transporting YopH through the needle structure to the host cell.
A team of researchers led by professor Magnus Wolf-Watz at the Department of Chemistry, Umeå University, has now discovered how the chaperone binding part of YopH recognizes and interacts with the protein SycH.
SycH is a chaperone whose task is to enable YopH to be transported through the needle structure and into the host cell. The research team has discovered that the chaperone binding part of YopH must completely loose its three dimensional structure to be able to grasp around the SycH protein like a horse’s shoe.
”This type of mechanism for protein-protein interaction can be called ”coupled folding and binding” and has not been seen before. Through this discovery we have contributed to a basic understanding of protein-protein interactions” says Magnus Wolf-Watz. ”This is important because many functions inside cells are carried out by protein-protein complexes.”
The discovery became possibly by Magnus Wolf-Watz putting together a team of researchers from different countries and with different special competence. The team consisted of group leaders Anders Hofer of Umeå University (expert in determining stoichiometry in protein complexes), Alexander Schug, Karlsruhe Institute of Technology (expert in modelling protein structures), Dmitri Svergun, EMBL, Hamburg (expert in protein structure determination with SAXS methodology) and Andrew Baldwin, Oxford University (expert in measuring relaxation with NMR spectroscopy). Experimentally the study was led by Arun Gupta, former post doc in Magnus Wolf-Watz’s research group.
For more information, please contact:
Magnus Wolf-Watz, professor, Department of Chemistry, Umeå University
Gupta, A., Reinartz, I., Karunanithy, G., Spilotros, A., Jonna, V.R.,Hofer, A., Svergun, D., Baldwin, A., Schug, A., and Wolf-Watz, M, Formation of a secretion competent protein complex by a dynamic wrap-around binding mechanism, Journal of Molecular Biology, Volume 430, Issue 18, Part B, 14 September 2018, DOI 10.1016/j.jmb.2018.07.014
Communications Department | idw - Informationsdienst Wissenschaft
Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung
Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.
Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
16.11.2018 | Health and Medicine
16.11.2018 | Life Sciences
16.11.2018 | Life Sciences