Researchers at Umeå University, Sweden, in collaboration with an international team, have discovered a new mechanism for interaction between two proteins that are vital for the Yersinia pseudotuberculosis bacteria’s pathogenic ability.
A common strategy bacteria have to cause disease is to transfer toxic proteins to host cells, for example in humans. Yersinia pseudotuberculosis is a bacteria that can cause gastrointestinal infections. The bacteria transfers proteins called Yop (Yersinia outer protein) through a complex needle structure forming a pore in the host cell membrane.
Yop proteins are made up of segments with different functions. The YopH protein has a segment which counteracts the immun system of the host cells. Another segment binds to chaperones, a group of proteins that help other proteins uphold a correct structure, which is important for transporting YopH through the needle structure to the host cell.
A team of researchers led by professor Magnus Wolf-Watz at the Department of Chemistry, Umeå University, has now discovered how the chaperone binding part of YopH recognizes and interacts with the protein SycH.
SycH is a chaperone whose task is to enable YopH to be transported through the needle structure and into the host cell. The research team has discovered that the chaperone binding part of YopH must completely loose its three dimensional structure to be able to grasp around the SycH protein like a horse’s shoe.
”This type of mechanism for protein-protein interaction can be called ”coupled folding and binding” and has not been seen before. Through this discovery we have contributed to a basic understanding of protein-protein interactions” says Magnus Wolf-Watz. ”This is important because many functions inside cells are carried out by protein-protein complexes.”
The discovery became possibly by Magnus Wolf-Watz putting together a team of researchers from different countries and with different special competence. The team consisted of group leaders Anders Hofer of Umeå University (expert in determining stoichiometry in protein complexes), Alexander Schug, Karlsruhe Institute of Technology (expert in modelling protein structures), Dmitri Svergun, EMBL, Hamburg (expert in protein structure determination with SAXS methodology) and Andrew Baldwin, Oxford University (expert in measuring relaxation with NMR spectroscopy). Experimentally the study was led by Arun Gupta, former post doc in Magnus Wolf-Watz’s research group.
For more information, please contact:
Magnus Wolf-Watz, professor, Department of Chemistry, Umeå University
Gupta, A., Reinartz, I., Karunanithy, G., Spilotros, A., Jonna, V.R.,Hofer, A., Svergun, D., Baldwin, A., Schug, A., and Wolf-Watz, M, Formation of a secretion competent protein complex by a dynamic wrap-around binding mechanism, Journal of Molecular Biology, Volume 430, Issue 18, Part B, 14 September 2018, DOI 10.1016/j.jmb.2018.07.014
Communications Department | idw - Informationsdienst Wissenschaft
Turning carbon dioxide into liquid fuel
06.08.2020 | DOE/Argonne National Laboratory
Tellurium makes the difference
06.08.2020 | Friedrich-Schiller-Universität Jena
Scientists at the Fraunhofer Institute for Laser Technology ILT have come up with a striking new addition to contact stamping technologies in the ERDF research project ScanCut. In collaboration with industry partners from North Rhine-Westphalia, the Aachen-based team of researchers developed a hybrid manufacturing process for the laser cutting of thin-walled metal strips. This new process makes it possible to fabricate even the tiniest details of contact parts in an eco-friendly, high-precision and efficient manner.
Plug connectors are tiny and, at first glance, unremarkable – yet modern vehicles would be unable to function without them. Several thousand plug connectors...
An international research team has found a new approach that may be able to reduce bone loss in osteoporosis and maintain bone health.
Osteoporosis is the most common age-related bone disease which affects hundreds of millions of individuals worldwide. It is estimated that one in three women...
Traditional single-cell sequencing methods help to reveal insights about cellular differences and functions - but they do this with static snapshots only...
“Core-shell” clusters pave the way for new efficient nanomaterials that make catalysts, magnetic and laser sensors or measuring devices for detecting electromagnetic radiation more efficient.
Whether in innovative high-tech materials, more powerful computer chips, pharmaceuticals or in the field of renewable energies, nanoparticles – smallest...
An international research team with Prof. Cornelia Denz from the Institute of Applied Physics at the University of Münster develop for the first time light fields using caustics that do not change during propagation. With the new method, the physicists cleverly exploit light structures that can be seen in rainbows or when light is transmitted through drinking glasses.
Modern applications as high resolution microsopy or micro- or nanoscale material processing require customized laser beams that do not change during...
23.07.2020 | Event News
21.07.2020 | Event News
07.07.2020 | Event News
06.08.2020 | Earth Sciences
06.08.2020 | Power and Electrical Engineering
06.08.2020 | Life Sciences