Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Pond-dwelling powerhouse's genome points to its biofuel potential

19.02.2014
Duckweed is a tiny floating plant that's been known to drive people daffy.

It's one of the smallest and fastest-growing flowering plants that often becomes a hard-to-control weed in ponds and small lakes. But it's also been exploited to clean contaminated water and as a source to produce pharmaceuticals.


Duckweed is a relatively simple plant with fronds that float on the surface of the water and roots that extend into the water. In the flask on the left, you can see the dormant phase, turions, that have dropped to the bottom.

Credit: Wenquin Wang

Now, the genome of Greater Duckweed (Spirodela polyrhiza) has given this miniscule plant's potential as a biofuel source a big boost. In a paper published February 19, 2014 in the journal Nature Communications, researchers from Rutgers University, the Department of Energy Joint Genome Institute and several other facilities detailed the complete genome of S. polyrhiza and analyzed it in comparison to several other plants, including rice and tomatoes.

Simple and primitive, a duckweed plant consists of a single small kidney-shaped leaf about the size of a pencil-top eraser that floats on the surface of the water with a few thin roots underwater. It grows in almost all geographic areas, at nearly any altitude. Although it's a flowering plant, it only rarely forms small indistinct flowers on the underside of its floating leaves. Most of the time, it reproduces by budding off small leaves that are clones of the parent leaf. It often forms thick mats on the edges of ponds, quiet inlets of lakes and in marshes. It's among the fastest growing plants, able to double its population in a couple of days under ideal conditions.

These and other properties make it an ideal candidate as a biofuel feedstock – a raw source for biofuel production. For example, unlike plants on land, duckweeds don't need to hold themselves upright or transport water from distant roots to their leaves, so they're a relatively soft and pliable plant, containing tiny amounts of woody material such as lignin and cellulose. Removing these woody materials from feedstock has been a major challenge in biofuel production. Also, although they are small enough to grow in many environments, unlike biofuel-producing microbes, duckweed plants are large enough to harvest easily.

S. polyrhiza turns out to have one of the smallest known plant genomes, at about 158 million base pairs and fewer than 20,000 protein-encoding genes. That's 27 percent fewer than Arabidopsis thaliana – which, until recently, was believed to be the smallest plant genome – and nearly half as many as rice plants.

"The most surprising find was insight into the molecular basis for genes involved in maturation – a forever-young lifestyle," said senior author Joachim Messing, director of the Waksman Institute of Microbiology at Rutgers University.

S. polyrhiza leaves resemble cotyledons, embryonic leaves inside plant seeds that become the first leaves after germination. But where other plants develop other kinds of leaves as they mature, S. polyrhiza's never progresses and continuously produces cotyledon leaves. This prolonging of juvenile traits is called "neoteny." S. polyrhiza had fewer genes to promote and more genes to repress the switch from juvenile to mature growth.

"Because of the reduction in neoteny, there is an arrest in development and differentiation of organs. So this arrest allowed us to uncover regulatory networks that are required for differentiation and development," Messing said.

Also intriguing to the research team were which genes were preserved over time and which were not. Many of the genes responsible for cellulose and lignin production in land dwelling plants were missing, and there were fewer copies of those that were present. Genes for another compound related to cell walls called "expansins" which are involved with cell wall and root growth were also reduced.

Genes for starch production, on the other hand, were retained and are probably used for creating starch-filled turions, specialized buds produced by aquatic plants for overwintering, enabling them sink to the bottom of ponds and revive in warmer weather. Moreover, despite the reduced number of total genes, S. polyrhiza has more copies of genes for enzymes involved in nitrogen absorption and metabolism than in other plants. This is probably linked to the plant's ability to utilize excess nitrogen in contaminated waters.

A thorough understanding of the genome and cellular mechanisms of S. polyrhiza could greatly enhance current efforts to recruit duckweed as a biofuel source. Messing estimates that duckweed will be a viable biofuel source within the next five years and points to Ceres Energy Group in New Jersey, which is already producing electricity from duckweed. Understanding which genes produce which traits will allow researchers to create new varieties of duckweed with enhanced biofuel traits, such as increased reduction of cellulose or increased starch or even higher lipid production. Starch can be directly used as a biofuel source and it can be converted to ethanol, the way corn is currently converted to ethanol fuel, but oils would have greater energy than ethanol.

"Classical breeding or genetics does not apply here because of its clonal propagation and rare flowering, but these organisms can be transformed with DNA," Messing said. "Therefore, new variants can be created with modified pathways for industrial applications. These variants would be an enhancement over what can be done now."

This genome was sequenced as part of a DOE Office of Science JGI Community Science Program (CSP) project (formerly the Community Sequencing Program). It exemplifies the collaborative approach and innovative projects that the CSP enables among researchers. Messing pointed to the study's advances over previous research.

"The sequencing of this genome opens new frontiers in the molecular biology of aquatic plants," said Messing. "This publication represents the single largest advance in this field and a new milestone in plant molecular biology and evolution, as previous studies were either classical botany or biochemistry of photosynthesis. The placement of the Spirodela genome as a basal monocot species will serve as a new reference for all flowering plants."

The authors on the publication also include researchers from MIPS/IBIS, Helmholtz Center Munich, Germany; University of California, Davis; Georgia Institute of Technology; Brookhaven National Laboratory; Donald Danforth Plant Science Center; University of Jena, Germany, HudsonAlpha Institute for Biotechnology; and the Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Germany.

The DOE Joint Genome Institute has announced a new call for letters of intent for the 2015 Community Science Program, due April 10, 2014. Details of the 2015 CSP call can be found at: http://bit.ly/CSP-15.

The U.S. Department of Energy Joint Genome Institute, supported by the DOE Office of Science, is committed to advancing genomics in support of DOE missions related to clean energy generation and environmental characterization and cleanup. DOE JGI, headquartered in Walnut Creek, Calif., provides integrated high-throughput sequencing and computational analysis that enable systems-based scientific approaches to these challenges. Follow @doe_jgi on Twitter.

DOE's Office of Science is the largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.

David Gilbert | EurekAlert!
Further information:
http://www.lbl.gov

More articles from Life Sciences:

nachricht Lateral gene transfer enables chemical protection of beetles against antagonistic fungi
18.07.2018 | Johannes Gutenberg-Universität Mainz

nachricht World’s Largest Study on Allergic Rhinitis Reveals new Risk Genes
17.07.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

Im Focus: Chemical reactions in the light of ultrashort X-ray pulses from free-electron lasers

Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.

Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

Microscopic trampoline may help create networks of quantum computers

17.07.2018 | Information Technology

In borophene, boundaries are no barrier

17.07.2018 | Materials Sciences

The role of Sodium for the Enhancement of Solar Cells

17.07.2018 | Power and Electrical Engineering

VideoLinks
Science & Research
Overview of more VideoLinks >>>