Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Observing molecules at the nano scale

12.05.2011
New impulses with the super-resolution microscopy for the University of Rostock

Scientists at the Reference- and Translation Center for Cardiac Stem Cell Therapy at the University of Rostock (RTC) are now able to visualize molecules with a microscope system with a localization accuracy of up to 10 nm.


Legend: Evgenya Delyagina, researcher at the RTC Rostock, will examine genetically modified stem cells with the help of the new microscope ELYRA PS.1. Photo: © Zeiss/RTC

This is achieved with a new product from Carl Zeiss MicroImaging GmbH, the microscope ELYRA PS.1. Besides Osaka the RTC Rostock is going to be the first user worldwide of a series system based on this technology. The project is funded by the Federal Ministry of Education and Research (BMBF).

„Our research deals with the efficacy, safety, and the further development of stem cell therapies against heart diseases. ELYRA will support our research “ Prof. Gustav Steinhoff, head of the RTC Rostock, explains. This microscope offers new interesting possibilities, especially for gene-technical and nano-technological approaches with stem cells. To improve the therapeutical efficacy or the survivability after transplantation the cells are genetically modified by using gene transfer systems. The gene transfer is currently conducted with a novel method whose mechanism has not been fully understood yet. Neither, it is known whether the modified cells are exposed to unexpected side effects.

With ELYRA PS.1, cell processes in live cells can now be observed and documented on a molecular level for the first time. Before that, it had been possible to image cellular structures with electron microscopy with a spatial resolution of up to 10 nanometers; however, living cells were destroyed in the course of the imaging procedure.

Combining two innovative technologies, SR-SIM und PALM, the ELYRA PS.1 is able to provide super-resolution which has not been available in the fluorescence microscopy technology until today. A doubling of the resolution of a conventional fluorescence microscopy has been achieved with the SR-SIM (Super-resolution Structured Illumination Microscopy) technology.

The necessary labeling can be performed with all common fluorescent dyes. The PALM (Photoactivated Localization Microscopy) technology offers a localization accuracy of up to 10 nm using e.g. shiftable fluorescent proteins. The ELYRA PS.1 at the RTC Rostock combines the super-resolution technology with the LSM 780, which is the latest and most effective confocal Laser Scanning Microscope from Carl Zeiss.

contact:
University of Rostock
Prof. Dr. Gustav Steinhoff
Center for Cardiac Stem Cell Therapy at the University of Rostock (RTC), Director
Fon: +49 (0)381 494 61 00
Mail: gustav.steinhoff@med.uni-rostock.de
University of Rostock
Press+Communication
Dr. Ulrich Vetter
Fon: +49 (0)381 498 1013
Mail: ulrich.vetter@uni-rostock.de

Ingrid Rieck | idw
Further information:
http://www.uni-rostock.de

More articles from Life Sciences:

nachricht Brought to light – chromobodies reveal changes in endogenous protein concentration in living cells
21.09.2018 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen

nachricht A one-way street for salt
21.09.2018 | Julius-Maximilians-Universität Würzburg

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists present new observations to understand the phase transition in quantum chromodynamics

The building blocks of matter in our universe were formed in the first 10 microseconds of its existence, according to the currently accepted scientific picture. After the Big Bang about 13.7 billion years ago, matter consisted mainly of quarks and gluons, two types of elementary particles whose interactions are governed by quantum chromodynamics (QCD), the theory of strong interaction. In the early universe, these particles moved (nearly) freely in a quark-gluon plasma.

This is a joint press release of University Muenster and Heidelberg as well as the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt.

Then, in a phase transition, they combined and formed hadrons, among them the building blocks of atomic nuclei, protons and neutrons. In the current issue of...

Im Focus: Patented nanostructure for solar cells: Rough optics, smooth surface

Thin-film solar cells made of crystalline silicon are inexpensive and achieve efficiencies of a good 14 percent. However, they could do even better if their shiny surfaces reflected less light. A team led by Prof. Christiane Becker from the Helmholtz-Zentrum Berlin (HZB) has now patented a sophisticated new solution to this problem.

"It is not enough simply to bring more light into the cell," says Christiane Becker. Such surface structures can even ultimately reduce the efficiency by...

Im Focus: New soft coral species discovered in Panama

A study in the journal Bulletin of Marine Science describes a new, blood-red species of octocoral found in Panama. The species in the genus Thesea was discovered in the threatened low-light reef environment on Hannibal Bank, 60 kilometers off mainland Pacific Panama, by researchers at the Smithsonian Tropical Research Institute in Panama (STRI) and the Centro de Investigación en Ciencias del Mar y Limnología (CIMAR) at the University of Costa Rica.

Scientists established the new species, Thesea dalioi, by comparing its physical traits, such as branch thickness and the bright red colony color, with the...

Im Focus: New devices based on rust could reduce excess heat in computers

Physicists explore long-distance information transmission in antiferromagnetic iron oxide

Scientists have succeeded in observing the first long-distance transfer of information in a magnetic group of materials known as antiferromagnets.

Im Focus: Finding Nemo's genes

An international team of researchers has mapped Nemo's genome

An international team of researchers has mapped Nemo's genome, providing the research community with an invaluable resource to decode the response of fish to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

"Boston calling": TU Berlin and the Weizenbaum Institute organize a conference in USA

21.09.2018 | Event News

One of the world’s most prominent strategic forums for global health held in Berlin in October 2018

03.09.2018 | Event News

4th Intelligent Materials - European Symposium on Intelligent Materials

27.08.2018 | Event News

 
Latest News

Astrophysicists measure precise rotation pattern of sun-like stars for the first time

21.09.2018 | Physics and Astronomy

Brought to light – chromobodies reveal changes in endogenous protein concentration in living cells

21.09.2018 | Life Sciences

"Boston calling": TU Berlin and the Weizenbaum Institute organize a conference in USA

21.09.2018 | Event News

VideoLinks
Science & Research
Overview of more VideoLinks >>>