Freiburg researchers use artificial membranes to show how a particular protein reaches the mitochondria
Mitochondria serve as the powerhouses of the cell, converting the energy stored in foods into a form cells can use. When this important task fails, it can result in numerous diseases, particularly those affecting organs with a high energy consumption like the brain or the heart.
The Freiburg biochemistry professor Chris Meisinger, the Freiburg molecular medicine researcher Dr. Nora Vögtle, and the Freiburg pharmaceutical scientists Dr. Martin Holzer and Dr. Michael Keller have discovered a new import pathway proteins use to reach the mitochondria.
The common assumption among researchers up until now has been that proteins are always transported into the mitochondria via so-called import machines. The newly discovered import pathway, by contrast, is independent of the import machines. The research team published the study in the Journal of Cell Biology.
Mitochondria need more than 1000 different proteins to fulfill their vital tasks for the cells. Most of these proteins are produced in the cellular fluid and then imported into the mitochondria. The powerhouses of the cell have import machines in their membranes for this purpose.
These import machines, which are for their part also composed of various proteins, act as gatekeepers and sluices, allowing the mitochondria to import the new proteins they need from the cellular fluid.
The research team found a new import pathway for the protein Ugo1 that does not pass through the import machines. Ugo1 is localized in the outer membrane of mitochondria. The scientists succeeded in reconstructing the protein’s transport pathway in artificial membranes consisting of lipids, fat-like substances present in the membranes of mitochondria.
The import no longer functioned when the researchers constructed the artificial membrane without a particular lipid only present in small amounts, phosphatidic acid. Moreover, the scientists demonstrated that living cells with an elevated concentration of phosphatidic acid also contain a higher amount of Ugo1. “This study shows that contrary to what has previously been assumed, lipids can take on specific and active functions in the import of mitochondrial proteins,” says Chris Meisinger.
Chris Meisinger is a research group leader at the Institute of Biochemistry and Molecular Biology of the University of Freiburg as well as a member of the Freiburg Cluster of Excellence BIOSS Centre for Biological Signalling Studies. Nora Vögtle is a member of Meisinger’s research group. Martin Holzer and Michael Keller conduct research at the Institute of Pharmaceutical Technology and Biopharmacy of the University of Freiburg.
Vögtle, F.N., Keller, M., Taskin, A.A., Horvath, S.E., Guan, X.L., Prinz, C., Opalinska, M., Zorzin, C., van der Laan, M., Wenk, M.R., Schubert, R., Wiedemann, N., Holzer, M., and Meisinger, C. (2015). The fusogenic lipid phosphatidic acid promotes the biogenesis of mitochondrial outer membrane protein Ugo1. Journal of Cell Biology.
Prof. Dr. Chris Meisinger
Institute of Biochemistry and Molecular Biology
University of Freiburg
Phone: +49 (0)761 / 203 - 5287
Rudolf-Werner Dreier | idw - Informationsdienst Wissenschaft
Good preparation is half the digestion
15.11.2018 | Max-Planck-Institut für Stoffwechselforschung
How the gut ‘talks’ to brown fat
16.11.2018 | Technische Universität München
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly
The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
15.11.2018 | Earth Sciences
15.11.2018 | Physics and Astronomy
15.11.2018 | Physics and Astronomy