Researchers at the Luxembourg Centre for Systems Biomedicine (LCSB), of the University of Luxembourg, have, under Dr. Manuel Buttini, successfully measured metabolic profiles, or the metabolomes, of different brain regions, and their findings could help better understand neurodegenerative diseases. The metabolome represents all or at least a large part of the metabolites in a given tissue, and thus, it gives a snapshot of its physiology.
„Our results, obtained in the mouse, are promising“, says Manuel Buttini: “They open up new opportunities to better understand neurodegenerative diseases, such as Parkinson’s, and could offer new ways to intervene therapeutically. In addition, with the help of metabolic profiles, such as those we have measured, the efficacy of novel therapeutic interventions could be tested more efficiently than with more common approaches.“
The researchers have just published their results in the „American Journal of Pathology“ (Am J Pathol 2015, 185: 1-14; http://dx.doi.org/10.1016/j.ajpath.2015.02.016).
Neurodegenerative processes, such as those occurring in Parkinson’s disease, are characterized by pathological alterations of the brain cells: these cells lose their structure and function, a process that is accompanied by changes in their metabolism. Until now, most scientists have always focused on just one or a few aspects of the disease to better describe and understand the underlying mechanisms.
By analysing the whole metabolome however, LCSB researchers have realized a more global approach: they now can analyse hundreds of biomolecules, produced by nerve cells in upper, middle, and lower brain regions of the mouse. In the process, they not only look at healthy brains, but also at brains in which neurodegeneration occurs.
“To study the metabolite signatures of the brain, we used gas-chromatography coupled to mass spectrometry. This approach is particularly suitable for the analysis of samples from complex tissues“, explains Dr. Christian Jäger, one of the three main authors of the study. With metabolic studies, an area in which the LCSB is one of the worldwide leading institutions, one can assess known and still unknown biomolecules in tissue samples.
After the measurements, LCSB-researchers have used a bioinformatical approach known as Machine Learning to specifically derive the metabolic profile of each brain region. These efforts were spearheaded by Dr. Enrico Glaab, the second main author of the study. “We found that a multitude of different molecules together reflect a specific functional state of nerve cells in each brain region.“ By comparing their observations with microscopic analysis of pathologic processes in nerve cells, the LCSB researchers could show which particular metabolic profile is associated with the degeneration of these cells.
“It was clearly the joined efforts of experts from quite different fields, an interdisciplinary approach that is encouraged at LCSB, that made this study possible. In this case, experts in Neurobiology, Biochemistry, Molecular Biology, and Bioinformatics came together to enable the successful completion of the study“, says Dr. Alessandro Michelucci, the third main author of the study.
“Our observations are important, on the one hand, for paving the way for the discovery of novel therapeutic opportunities to combat neurodegeneration“, says Dr. Manuel Buttini, “and, on the other hand, for the development of new drugs to fight diseases such as Parkinson’s or Alzheimer’s. Indeed, by analysing metabolite profiles rather than just microscopic cellular changes or individual biomolecules, a better understanding of the effect of novel therapeutics for brain diseases should be feasible.“
http://www.sciencedirect.com/science/article/pii/S0002944015001509 - Link to the scientic publication
http://www.lcsb.lu - Homepage of the Luxembourg Centre for Systems Biomedicine
Britta Schlüter | Universität Luxemburg - Université du Luxembourg
Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung
Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.
Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
16.11.2018 | Health and Medicine
16.11.2018 | Life Sciences
16.11.2018 | Life Sciences