Using nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography, they have determined the 3-D structure and interactions of the histone chaperone Rtt106 down to the atomic details. The findings are published in the journal Nature.
“The interactions we described are important for gene silencing and the DNA damage response,” says senior author Georges Mer, Ph.D., a Mayo Clinic structural biologist. “This is exciting because our newfound knowledge will help us better understand these fundamental cellular processes.”
In cells, our DNA is part of a structure called chromatin, comprised of proteins, the majority called histones, which are wrapped with the DNA. Associated with the histones is another group of proteins called histone chaperones, which promote the proper assembly or disassembly of the chromatin during the times our DNA is replicated or repaired when damaged. Their dysfunction has been linked to cancer, aging and other diseases.
Mayo researchers discovered two novel domains in Rtt106 that take on this role. One, termed the homodimerization domain, allows two molecules of Rtt106 to be linked so they can cooperate in binding histones H3 and H4. The other, called the double PH domain, is responsible for sensing the acetylated lysine of H3 and further reinforces the interaction. The combined actions of the two domains of Rtt106 enable it to perform the chaperoning function efficiently and properly. This is the first time anyone has described this mode of specific association between a histone chaperone and a modified histone complex.
Other authors of the study are Dan Su, M.D., Ph.D.; Qi Hu, Ph.D.; Qing Li, Ph.D.; James Thompson, Ph.D.; Gaofeng Cui, Ph.D.; Ahmed Fazly, Ph.D.; Brian Davies, Ph.D.; Maria Victoria Botuyan, Ph.D.; and Zhiguo Zhang, Ph.D.; all of Mayo Clinic. The study was a collaborative effort between the groups of Dr. Mer and Dr. Zhang and was funded by the National Institutes of Health and Mayo Clinic.
Robert Nellis | Newswise Science News
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