During field studies, scientists at the Max Planck Institute for Chemical Ecology discovered that the oral secretions of tobacco hornworm larvae (Manduca sexta) contain a particular substance that promptly alters a green leaf volatile in tobacco leaves into an odor attractant signal. With this signal, called (E)-2-hexenal, they unintentionally lure their own enemies: carnivorous bugs. These bugs start their piercing attacks not only against freshly hatched caterpillar babies; they also devour eggs laid by the female moths. (SCIENCE, August 27, 2010)
Experimental setup in the field: eggs, a young caterpillar and a predatory bug that has just arrived, all are located on the bottom of the tobacco leaf. MPI chemische Ökologie / Danny Kessler
A freshly hatched tobacco hornworm larva (Manduca sexta) is attacked by a predatory bug of the genus Geocoris. Only a few hours before, the feeding caterpillar had – catalyzed by a substance in its oral secretion – changed the chemistry of the plant’s “green” odor in such a way as to produce the new odor which attracts its own enemy; the change is to the plant’s advantage as it has lethal consequences for the caterpillar. Matthey Film
Plants have developed a sophisticated defense system. They can not only directly fend off herbivores by producing toxins, but also do so indirectly by emitting odorant molecules into the atmosphere that are perceived by predatory insects; these predators are lured to the attacked plant and feed on the herbivore or parasitize it – thereby providing a benefit for the plant.
Since the discovery of indirect defense strategies in plants, ecologists have been interested in the molecular mechanisms and the course of events underlying them. For example, almost all plants switch on some of their defenses only when an herbivore is actually attacking them. But how does the plant recognize the attack from the herbivore? “The plant cannot see its attacker, but plants can sense the digestive substances that attacking larvae have in their oral secretions when these substances come into contact with the leaves,” Silke Allmann, PhD student from Dortmund in the Department of Molecular Ecology, headed by Prof. Ian Baldwin, assumes. Ian Baldwin’s department is looking for plant substances, signal transduction chains and metabolic pathways after herbivore attack.
Recently, the scientists’ attention was drawn to substances in tobacco plants called “green leaf volatiles” (GLV); GLV are compounds released by plants after mechanical damage. These molecules are responsible for the typical smell of freshly cut grass. Last year the green odorant substances of tobacco were examined more carefully. The scientists found that the amount of a certain GLV, namely (E)-2-hexenal, suddenly increased after oral secretions of Manduca sexta larvae had been applied on wounded leaves. In tobacco plants the aldehyde, hexanal, is present in two isomeric forms: (Z)-3-hexenal and (E)-2-hexenal. Z-E conversions can be catalyzed by specific enzymes, so-called isomerases. “We supposed that the increased occurrence of (E)-2-hexenal may attract the carnivores, because we always discovered Geocoris when young Manduca larvae had just hatched and started feeding on the tobacco leaves,” says Ian Baldwin.
To test this assumption, the researchers started a series of experiments using Manduca sexta eggs and different blends of (Z)-3-hexenal and (E)-2-hexenal. The eggs were glued to tobacco plants growing in the field and different mixtures of odorant signals were applied to adjacently located cotton swabs. After 12 and 24 hours, the fate of eggs on the leaves was determined. The result: Plants that had been perfumed with the Z-lure had only 8% of their glued eggs attacked, whereas plants perfumed with the E-lure had lost 24% of the eggs. And the eggs had been eaten by Geocoris. The speed of plants’ defense triggering was particularly impressive: it takes less than an hour to trigger the conversion of the Z-lure to the E-lure and less than 24 hours for the predation of the eggs. “Other indirect defense mechanisms of plants require the activation of new metabolic pathways for the release of odorant signals, and these responses are much slower,” says Ian Baldwin. The release of (E)-2-hexenal after its rapid and simple conversion from (Z)-3-hexenal is so rapid that it provides the prey-searching predator with enough information to pinpoint the exact location of the feeding herbivore on a plant. And it is this information which makes the release of volatile “alarm calls” an effective defense strategy for plants.
But which substance is betraying the caterpillar? “The simplest assumption is that the larval oral secretions contain a special enzyme, probably an isomerase, which rearranges the Z:E ratio of the aldehyde in favor of (E)-2-hexenal,” says Silke Allmann. To test this assumption, the oral secretion was briefly heated and subsequently applied on wounded leaves. (E)-2-hexenal was now no longer produced. Hence, the unknown substance is likely an enzyme, because enzymes are typically heat labile. The scientists also determined whether tobacco plants react specifically on Manduca sexta oral secretions or to secretions of other larval species as well. Interestingly, the secretions of two Spodopera species (Spodoptera exigua and S. littoralis) had significantly lower effects.
However, one question remains unanswered: Why does the oral secretion of Manduca sexta larvae contain a substance which endangers the larvae’s lives as soon as they start feeding on tobacco leaves? Silke Allmann and Ian Baldwin hypothesize that the production of (E)-2-hexenal protects the larvae against other untested attackers, such as bacteria. Harmful microorganisms, consumed by the larvae every day in large amounts together with the leaf tissue, may be killed quickly and effectively by (E)-2-hexenal, which is known to be a strong antibiotic agent. Hence the detrimental aspects of producing (E)-2-hexenal, that of betraying the precise location of the Manduca sexta larvae to their enemies, may be balanced by the benefits of fewer bacterial infections.
Regardless, the winner in this tritrophic food chain is the carnivore Geocoris, which is well fed as a result of the response – thanks to its highly sensitive antennae which enable it to smell the slightest concentrations of (E)-2-hexenal. [JWK, AO]Original Publication:
Silke Allmann, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany. Mobile: +31 (0)624 356988; firstname.lastname@example.orgPicture Requests:
Dr. Jan-Wolfhard Kellmann | Max-Planck-Institut
Brought to light – chromobodies reveal changes in endogenous protein concentration in living cells
21.09.2018 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen
A one-way street for salt
21.09.2018 | Julius-Maximilians-Universität Würzburg
The building blocks of matter in our universe were formed in the first 10 microseconds of its existence, according to the currently accepted scientific picture. After the Big Bang about 13.7 billion years ago, matter consisted mainly of quarks and gluons, two types of elementary particles whose interactions are governed by quantum chromodynamics (QCD), the theory of strong interaction. In the early universe, these particles moved (nearly) freely in a quark-gluon plasma.
This is a joint press release of University Muenster and Heidelberg as well as the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt.
Then, in a phase transition, they combined and formed hadrons, among them the building blocks of atomic nuclei, protons and neutrons. In the current issue of...
Thin-film solar cells made of crystalline silicon are inexpensive and achieve efficiencies of a good 14 percent. However, they could do even better if their shiny surfaces reflected less light. A team led by Prof. Christiane Becker from the Helmholtz-Zentrum Berlin (HZB) has now patented a sophisticated new solution to this problem.
"It is not enough simply to bring more light into the cell," says Christiane Becker. Such surface structures can even ultimately reduce the efficiency by...
A study in the journal Bulletin of Marine Science describes a new, blood-red species of octocoral found in Panama. The species in the genus Thesea was discovered in the threatened low-light reef environment on Hannibal Bank, 60 kilometers off mainland Pacific Panama, by researchers at the Smithsonian Tropical Research Institute in Panama (STRI) and the Centro de Investigación en Ciencias del Mar y Limnología (CIMAR) at the University of Costa Rica.
Scientists established the new species, Thesea dalioi, by comparing its physical traits, such as branch thickness and the bright red colony color, with the...
Scientists have succeeded in observing the first long-distance transfer of information in a magnetic group of materials known as antiferromagnets.
An international team of researchers has mapped Nemo's genome, providing the research community with an invaluable resource to decode the response of fish to...
21.09.2018 | Event News
03.09.2018 | Event News
27.08.2018 | Event News
21.09.2018 | Physics and Astronomy
21.09.2018 | Life Sciences
21.09.2018 | Event News