Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Hazel, birch or grass? - Distinguish pollen using microfluidics and neuronal networks

09.08.2018

A miniaturized lab on a chip enables high resolution microscopy images of several thousands of pollen particles in seconds. Neuronal networks take over the image processing and classify the particles fast and reliable. Andreas Kleiber, PhD student at the Leibniz-Institute of Photonic Technology in Jena (Leibniz IPHT), tested the method on several highly allergenic pollen. For his results, presented during the 3rd Imaging Technology Summer Workshop dedicated to Big Data in Imaging, Kleiber was awarded the poster price by the European Society for Molecular Imaging.

Up to 1000 pollen per second flow by the optical window in a narrow channel on the stamp sized chip. A digital camera captures each of the tiny single grains through a microscope lens.


The patented design of the microfluidic channels allows to align all particles in the focal plane.

Source: A. Kleiber/Leibniz-IPHT


Birch pollen is one of eight different types of pollen, investigated by Andreas Kleiber.

Picture: designed by Bearfotos - Freepik.com

To receive sharp shots for the following data processing, every analyzed particle has to pass the liquid channel in the focal plane of the lens. The height of the focal plane of the used high-resolution lenses measures less than a hundredth millimetre.

Scientists of Leibniz IPHT met this technological challenge employing a sophisticated design of the components in the microfluidic chip. The patented method enables them to align the pollen grains exactly in the focal plane and therefore to obtain sharp images of all objects.

"Using two liquid streams from the sides, we press the particle stream to a sheet, just like a nozzle. A new arrangement of the micro channels rotates the sheet by 90° into the focal plane,“ explains Andreas Kleiber the technology. in the scope of his PhD thesis, the scientist researches methods for the high-throughput-analysis of bioparticles using microfluidic chips. 

The principle of hydrodynamic focusing is already known in the field of flow cytometry for the analysis of cell populations. Here, the cells are focused in a way that they pass by the measurement window along a line. "New to our system is, that we arrange the particles in a thin, two dimensional lamella, and therefore use the whole frame of the camera. This makes the method so rapid“, says Kleiber.

The researchers can actuate the horizontal position and thickness of the particle layer accurately. Therefore, they are able to control the rotation of the pollen in the stream. "Using methods already known from computer-tomography we are able to produce 3D-image data that contain important information e.g. about the three-dimensional morphology of a pollen grain.

The 3D-information improves the reliability of the pollen identification significantly“, elucidated Kleiber. The researcher evaluates images of the different pollen with software tools for particle tracking and feature extraction. A pre-trained convolutional neuronal network classifies the shots to a certain kind of pollen by means of the extracted data. The hit rate is above 98%.

The researchers classified the pollen, which originate from the research group Indoor Climatology at the University Hospital Jena, without any additional label, solely on basis of the image information from microscopy. "We are able to use the method furthermore for the analysis of cells e.g. to distinguish subtypes of white blood cells“, underlines Dr. Thomas Henkel, who leads the relevant research work at Leibniz IPHT.

"In the future, it should be possible to sort bioparticles with our chip“, says Henkel about the planned research, which is funded by the EU in the range of the Era-NET-DLR project "WaterChip“.

Wissenschaftliche Ansprechpartner:

Andreas Kleiber
Work Group Microfluidics//Leibniz IPHT Jena
Andreas.Kleiber(a)leibniz-ipht.de
+49 (0) 3641 206-357

Dr. Thomas Henkel
Work Group Leader
Thomas.Henkel(a)leibniz-ipht.de
+49 (0) 3641 206-307

Weitere Informationen:

https://www.leibniz-ipht.de/en/institute/presse/news/detail/hasel-birke-oder-gra...
http://www.e-smi.eu/index.php?id=topim-tech-20180
http://waterchip.eu

Dr. Anja Schulz | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht New technique to determine protein structures may solve biomedical puzzles
11.12.2019 | Dana-Farber Cancer Institute

nachricht NTU Singapore scientists convert plastics into useful chemicals using su
11.12.2019 | Nanyang Technological University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Highly charged ion paves the way towards new physics

In a joint experimental and theoretical work performed at the Heidelberg Max Planck Institute for Nuclear Physics, an international team of physicists detected for the first time an orbital crossing in the highly charged ion Pr⁹⁺. Optical spectra were recorded employing an electron beam ion trap and analysed with the aid of atomic structure calculations. A proposed nHz-wide transition has been identified and its energy was determined with high precision. Theory predicts a very high sensitivity to new physics and extremely low susceptibility to external perturbations for this “clock line” making it a unique candidate for proposed precision studies.

Laser spectroscopy of neutral atoms and singly charged ions has reached astonishing precision by merit of a chain of technological advances during the past...

Im Focus: Ultrafast stimulated emission microscopy of single nanocrystals in Science

The ability to investigate the dynamics of single particle at the nano-scale and femtosecond level remained an unfathomed dream for years. It was not until the dawn of the 21st century that nanotechnology and femtoscience gradually merged together and the first ultrafast microscopy of individual quantum dots (QDs) and molecules was accomplished.

Ultrafast microscopy studies entirely rely on detecting nanoparticles or single molecules with luminescence techniques, which require efficient emitters to...

Im Focus: How to induce magnetism in graphene

Graphene, a two-dimensional structure made of carbon, is a material with excellent mechanical, electronic and optical properties. However, it did not seem suitable for magnetic applications. Together with international partners, Empa researchers have now succeeded in synthesizing a unique nanographene predicted in the 1970s, which conclusively demonstrates that carbon in very specific forms has magnetic properties that could permit future spintronic applications. The results have just been published in the renowned journal Nature Nanotechnology.

Depending on the shape and orientation of their edges, graphene nanostructures (also known as nanographenes) can have very different properties – for example,...

Im Focus: Electronic map reveals 'rules of the road' in superconductor

Band structure map exposes iron selenide's enigmatic electronic signature

Using a clever technique that causes unruly crystals of iron selenide to snap into alignment, Rice University physicists have drawn a detailed map that reveals...

Im Focus: Developing a digital twin

University of Texas and MIT researchers create virtual UAVs that can predict vehicle health, enable autonomous decision-making

In the not too distant future, we can expect to see our skies filled with unmanned aerial vehicles (UAVs) delivering packages, maybe even people, from location...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

The Future of Work

03.12.2019 | Event News

First International Conference on Agrophotovoltaics in August 2020

15.11.2019 | Event News

Laser Symposium on Electromobility in Aachen: trends for the mobility revolution

15.11.2019 | Event News

 
Latest News

Self-driving microrobots

11.12.2019 | Materials Sciences

Innovation boost for “learning factory”: European research project “SemI40” generates path-breaking findings

11.12.2019 | Information Technology

Molecular milk mayonnaise: How mouthfeel and microscopic properties are related in mayonnaise

11.12.2019 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>