Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Hazel, birch or grass? - Distinguish pollen using microfluidics and neuronal networks

09.08.2018

A miniaturized lab on a chip enables high resolution microscopy images of several thousands of pollen particles in seconds. Neuronal networks take over the image processing and classify the particles fast and reliable. Andreas Kleiber, PhD student at the Leibniz-Institute of Photonic Technology in Jena (Leibniz IPHT), tested the method on several highly allergenic pollen. For his results, presented during the 3rd Imaging Technology Summer Workshop dedicated to Big Data in Imaging, Kleiber was awarded the poster price by the European Society for Molecular Imaging.

Up to 1000 pollen per second flow by the optical window in a narrow channel on the stamp sized chip. A digital camera captures each of the tiny single grains through a microscope lens.


The patented design of the microfluidic channels allows to align all particles in the focal plane.

Source: A. Kleiber/Leibniz-IPHT


Birch pollen is one of eight different types of pollen, investigated by Andreas Kleiber.

Picture: designed by Bearfotos - Freepik.com

To receive sharp shots for the following data processing, every analyzed particle has to pass the liquid channel in the focal plane of the lens. The height of the focal plane of the used high-resolution lenses measures less than a hundredth millimetre.

Scientists of Leibniz IPHT met this technological challenge employing a sophisticated design of the components in the microfluidic chip. The patented method enables them to align the pollen grains exactly in the focal plane and therefore to obtain sharp images of all objects.

"Using two liquid streams from the sides, we press the particle stream to a sheet, just like a nozzle. A new arrangement of the micro channels rotates the sheet by 90° into the focal plane,“ explains Andreas Kleiber the technology. in the scope of his PhD thesis, the scientist researches methods for the high-throughput-analysis of bioparticles using microfluidic chips. 

The principle of hydrodynamic focusing is already known in the field of flow cytometry for the analysis of cell populations. Here, the cells are focused in a way that they pass by the measurement window along a line. "New to our system is, that we arrange the particles in a thin, two dimensional lamella, and therefore use the whole frame of the camera. This makes the method so rapid“, says Kleiber.

The researchers can actuate the horizontal position and thickness of the particle layer accurately. Therefore, they are able to control the rotation of the pollen in the stream. "Using methods already known from computer-tomography we are able to produce 3D-image data that contain important information e.g. about the three-dimensional morphology of a pollen grain.

The 3D-information improves the reliability of the pollen identification significantly“, elucidated Kleiber. The researcher evaluates images of the different pollen with software tools for particle tracking and feature extraction. A pre-trained convolutional neuronal network classifies the shots to a certain kind of pollen by means of the extracted data. The hit rate is above 98%.

The researchers classified the pollen, which originate from the research group Indoor Climatology at the University Hospital Jena, without any additional label, solely on basis of the image information from microscopy. "We are able to use the method furthermore for the analysis of cells e.g. to distinguish subtypes of white blood cells“, underlines Dr. Thomas Henkel, who leads the relevant research work at Leibniz IPHT.

"In the future, it should be possible to sort bioparticles with our chip“, says Henkel about the planned research, which is funded by the EU in the range of the Era-NET-DLR project "WaterChip“.

Wissenschaftliche Ansprechpartner:

Andreas Kleiber
Work Group Microfluidics//Leibniz IPHT Jena
Andreas.Kleiber(a)leibniz-ipht.de
+49 (0) 3641 206-357

Dr. Thomas Henkel
Work Group Leader
Thomas.Henkel(a)leibniz-ipht.de
+49 (0) 3641 206-307

Weitere Informationen:

https://www.leibniz-ipht.de/en/institute/presse/news/detail/hasel-birke-oder-gra...
http://www.e-smi.eu/index.php?id=topim-tech-20180
http://waterchip.eu

Dr. Anja Schulz | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht Molecular 'clutch' puts infection-fighting cells into gear
22.07.2019 | National Centre for Biological Sciences

nachricht Living Components
22.07.2019 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: MOF@SAW: Nanoquakes and molecular sponges for weighing and separating tiny masses

Augsburg chemists and physicists report how they have succeeded in the extremely difficult separation of hydrogen and deuterium in a gas mixture.

Thanks to the Surface Acoustic Wave (SAW) technology developed here and already widely used, the University of Augsburg is internationally recognized as the...

Im Focus: Better thermal conductivity by adjusting the arrangement of atoms

Adjusting the thermal conductivity of materials is one of the challenges nanoscience is currently facing. Together with colleagues from the Netherlands and Spain, researchers from the University of Basel have shown that the atomic vibrations that determine heat generation in nanowires can be controlled through the arrangement of atoms alone. The scientists will publish the results shortly in the journal Nano Letters.

In the electronics and computer industry, components are becoming ever smaller and more powerful. However, there are problems with the heat generation. It is...

Im Focus: First-ever visualizations of electrical gating effects on electronic structure

Scientists have visualised the electronic structure in a microelectronic device for the first time, opening up opportunities for finely-tuned high performance electronic devices.

Physicists from the University of Warwick and the University of Washington have developed a technique to measure the energy and momentum of electrons in...

Im Focus: Megakaryocytes act as „bouncers“ restraining cell migration in the bone marrow

Scientists at the University Würzburg and University Hospital of Würzburg found that megakaryocytes act as “bouncers” and thus modulate bone marrow niche properties and cell migration dynamics. The study was published in July in the Journal “Haematologica”.

Hematopoiesis is the process of forming blood cells, which occurs predominantly in the bone marrow. The bone marrow produces all types of blood cells: red...

Im Focus: Artificial neural network resolves puzzles from condensed matter physics: Which is the perfect quantum theory?

For some phenomena in quantum many-body physics several competing theories exist. But which of them describes a quantum phenomenon best? A team of researchers from the Technical University of Munich (TUM) and Harvard University in the United States has now successfully deployed artificial neural networks for image analysis of quantum systems.

Is that a dog or a cat? Such a classification is a prime example of machine learning: artificial neural networks can be trained to analyze images by looking...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on UV LED Technologies & Applications – ICULTA 2020 | Call for Abstracts

24.06.2019 | Event News

SEMANTiCS 2019 brings together industry leaders and data scientists in Karlsruhe

29.04.2019 | Event News

Revered mathematicians and computer scientists converge with 200 young researchers in Heidelberg!

17.04.2019 | Event News

 
Latest News

Toward molecular computers: First measurement of single-molecule heat transfer

22.07.2019 | Information Technology

First impressions go a long way in the immune system

22.07.2019 | Health and Medicine

New Record: PLQE of 70.3% in lead-free halide double perovskites

22.07.2019 | Power and Electrical Engineering

VideoLinks
Science & Research
Overview of more VideoLinks >>>