Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Harvard Wyss Institute researchers demonstrate machine-guided engineering of AAV capsids

29.11.2019

High-throughput synthetic biology approach reveals hidden AAV features and could help fast-track future gene therapies

Adeno-associated viruses (AAVs) have become the go-to vehicle for delivering therapeutic gene cargo to target tissues for the recent wave of gene therapies that are in development in academic and biotechnology laboratories. However, natural AAVs do not specifically target diseased cells and tissues, and they can be recognized by the immune system in ways that limit therapeutic success. To improve AAVs, synthetic biologists have been taking a "directed evolution" approach in which they randomly mutate specific amino acid building blocks of the capsid proteins that form the shell of the virus and directly contact target cells. By evaluating which changes can route the virus to target tissues and successively layering mutations on top of each other in an arduous iterative process, they aim to improve desirable AAV traits.


In this photo Sam Sinai, George Church, Eric Kelsic, and Pierce Ogden are holden small models of the AVVs capsid in their hands.

Credit: Wyss Institute at Harvard University

Now scientists at Harvard's Wyss Institute for Biologically Inspired Engineering and Harvard Medical School (HMS) report an approach to speed up the process of making such enhanced AAV capsids, and to develop even better viruses.

Taking a different, more systematic approach to the capsid protein-engineering problem, the team mutated one by one each of the 735 amino acids within the AAV2 capsid, the best-known member of the AAV family, including all possible codon substitutions, insertions and deletions at each position. They generated a virus library containing about 200,000 variants and identified capsid changes that both maintained AAV2's viability and improved its "homing" potential (tropism) to specific organs in mice. Unexpectedly, the team also discovered a new accessory protein hidden within the capsid-encoding DNA sequence that binds to the membrane of target cells. Their findings are reported in Science.

... more about:
»HMS »synthetic

The team led by Wyss Core Faculty member George Church, Ph.D., and his former postdoctoral fellow Eric Kelsic, Ph.D., deployed an advanced synthetic biology armamentarium including next-generation DNA-synthesis, barcoding, and DNA sequencing capabilities for constructing one of the most comprehensive AAV capsid libraries to date. "With the information generated by this library, we were also able to design capsids with more mutations than previous natural or synthetic variants, and furthermore with efficiencies of generating viable capsids that far exceed those of AAV created by random mutagenesis approaches" said Church, who is a Lead of the Wyss Institute's Synthetic Biology platform, and also the Robert Winthrop Professor of Genetics at HMS and Professor of Health Sciences and Technology at Harvard University and the Massachusetts Institute of Technology (MIT).

"These high-throughput technologies paired with machine-guided design lay the foundation for engineering superior and highly tailored AAV variants for future gene therapies", said co-first author Eric Kelsic, Ph.D., who is now CEO of Dyno Therapeutics. "Past approaches such as rational design or random mutagenesis each had their drawbacks, either being limited in the library size or being low in quality, respectively. Machine-guided design is a data-driven approach to protein engineering. Here we show that even a simple mathematical model, powered by enough data, can successfully generate viable synthetic capsids. This iterative and empirical approach to protein engineering enables us to get the best of both worlds and generate large numbers of high quality capsid variants."

"Unexpectedly, the high-resolution data we generated enabled us to spot a new protein encoded by a different reading frame within the capsid's DNA sequence - which had escaped notice despite decades of intense research on the virus," said co-first author Pierce Ogden, Ph.D., a former graduate student and now postdoctoral fellow working with Church. "Membrane-associated accessory protein (MAAP), as we named it, exists in all of the most popular AAV serotypes and we believe that it plays a role in the virus' natural life cycle. Studying how MAAP functions will be an exciting area for future research and could potentially lead to a better understanding of how to better produce and engineer AAV gene therapies."

According to co-author Sam Sinai, Ph.D., a former graduate student of Church, now a Machine Learning Scientist at Dyno Therapeutics, "This reveals the promise of data-driven protein engineering, in particular for proteins like the AAV capsid that are difficult to model with current computational approaches. Our results are highly encouraging but also only a first step. Using this data and those from future experiments, we will be building machine learning models to optimize capsids and address a wide variety of gene therapy challenges."

Kelsic, Sinai and Church are co-founders of Dyno Therapeutics Inc., and all hold equity in the company.

"This study is a landmark in the Wyss Institute's Synthetic Biology platform's effort to advance AAV technology to the next level. This work is also a great example of how we are beginning to integrate machine learning and artificial intelligence approaches into our therapeutics pipeline," said Wyss Institute Founding Director Donald Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at HMS, the Vascular Biology Program at Boston Children's Hospital, and Professor of Bioengineering at Harvard's John A. Paulson School of Engineering and Applied Sciences (SEAS).

###

The study was funded by Harvard's Wyss Institute for Biologically Inspired Engineering and the National Institutes of Health.

PRESS CONTACT

Wyss Institute for Biologically Inspired Engineering at Harvard University Benjamin Boettner, benjamin.boettner@wyss.harvard.edu, +1 617-432-8232

MULTIMEDIA AVAILABLE

The Wyss Institute for Biologically Inspired Engineering at Harvard University uses Nature's design principles to develop bioinspired materials and devices that will transform medicine and create a more sustainable world. Wyss researchers are developing innovative new engineering solutions for healthcare, energy, architecture, robotics, and manufacturing that are translated into commercial products and therapies through collaborations with clinical investigators, corporate alliances, and formation of new startups. The Wyss Institute creates transformative technological breakthroughs by engaging in high risk research, and crosses disciplinary and institutional barriers, working as an alliance that includes Harvard's Schools of Medicine, Engineering, Arts & Sciences and Design, and in partnership with Beth Israel Deaconess Medical Center, Brigham and Women's Hospital, Boston Children's Hospital, Dana-Farber Cancer Institute, Massachusetts General Hospital, the University of Massachusetts Medical School, Spaulding Rehabilitation Hospital, Boston University, Tufts University, Charité - Universitätsmedizin Berlin, University of Zurich and Massachusetts Institute of Technology.

Harvard Medical School has more than 11,000 faculty working in 10 academic departments located at the School's Boston campus or in hospital-based clinical departments at 15 Harvard-affiliated teaching hospitals and research institutes: Beth Israel Deaconess Medical Center, Boston Children's Hospital, Brigham and Women's Hospital, Cambridge Health Alliance, Dana-Farber Cancer Institute, Harvard Pilgrim Health Care Institute, Hebrew SeniorLife, Joslin Diabetes Center, Judge Baker Children's Center, Massachusetts Eye and Ear/Schepens Eye Research Institute, Massachusetts General Hospital, McLean Hospital, Mount Auburn Hospital, Spaulding Rehabilitation Network and VA Boston Healthcare System.

Benjamin Boettner | EurekAlert!

Further reports about: HMS synthetic

More articles from Life Sciences:

nachricht When plants bloom
29.11.2019 | Max-Planck-Institut für Molekulare Pflanzenphysiologie

nachricht Harnessing the power of CRISPR in space and time
29.11.2019 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: How do scars form? Fascia function as a repository of mobile scar tissue

Abnormal scarring is a serious threat resulting in non-healing chronic wounds or fibrosis. Scars form when fibroblasts, a type of cell of connective tissue, reach wounded skin and deposit plugs of extracellular matrix. Until today, the question about the exact anatomical origin of these fibroblasts has not been answered. In order to find potential ways of influencing the scarring process, the team of Dr. Yuval Rinkevich, Group Leader for Regenerative Biology at the Institute of Lung Biology and Disease at Helmholtz Zentrum München, aimed to finally find an answer. As it was already known that all scars derive from a fibroblast lineage expressing the Engrailed-1 gene - a lineage not only present in skin, but also in fascia - the researchers intentionally tried to understand whether or not fascia might be the origin of fibroblasts.

Fibroblasts kit - ready to heal wounds

Im Focus: McMaster researcher warns plastic pollution in Great Lakes growing concern to ecosystem

Research from a leading international expert on the health of the Great Lakes suggests that the growing intensity and scale of pollution from plastics poses serious risks to human health and will continue to have profound consequences on the ecosystem.

In an article published this month in the Journal of Waste Resources and Recycling, Gail Krantzberg, a professor in the Booth School of Engineering Practice...

Im Focus: Machine learning microscope adapts lighting to improve diagnosis

Prototype microscope teaches itself the best illumination settings for diagnosing malaria

Engineers at Duke University have developed a microscope that adapts its lighting angles, colors and patterns while teaching itself the optimal...

Im Focus: Small particles, big effects: How graphene nanoparticles improve the resolution of microscopes

Conventional light microscopes cannot distinguish structures when they are separated by a distance smaller than, roughly, the wavelength of light. Superresolution microscopy, developed since the 1980s, lifts this limitation, using fluorescent moieties. Scientists at the Max Planck Institute for Polymer Research have now discovered that graphene nano-molecules can be used to improve this microscopy technique. These graphene nano-molecules offer a number of substantial advantages over the materials previously used, making superresolution microscopy even more versatile.

Microscopy is an important investigation method, in physics, biology, medicine, and many other sciences. However, it has one disadvantage: its resolution is...

Im Focus: Atoms don't like jumping rope

Nanooptical traps are a promising building block for quantum technologies. Austrian and German scientists have now removed an important obstacle to their practical use. They were able to show that a special form of mechanical vibration heats trapped particles in a very short time and knocks them out of the trap.

By controlling individual atoms, quantum properties can be investigated and made usable for technological applications. For about ten years, physicists have...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

First International Conference on Agrophotovoltaics in August 2020

15.11.2019 | Event News

Laser Symposium on Electromobility in Aachen: trends for the mobility revolution

15.11.2019 | Event News

High entropy alloys for hot turbines and tireless metal-forming presses

05.11.2019 | Event News

 
Latest News

When plants bloom

29.11.2019 | Life Sciences

Harnessing the power of CRISPR in space and time

29.11.2019 | Life Sciences

New evolutionary insights into the early development of songbirds

29.11.2019 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>