Defense mechanisms used by the fungus Cryptococcus neoformans enable it to lead to fatal meningitis, which is one of the opportunistic infections often associated with death in HIV/AIDS patients, organ transplant recipients, diabetics and other immunosuppressed patients. In describing the complex process of how C. neoformans averts destruction in the lungs of mice, the Duke researchers have opened new options for drug development.
"Very few antifungal drugs are effective, so we need to identify the Achilles' heel of these fungal pathogens," said Dennis J. Thiele, PhD, the George Barth Geller Professor of Pharmacology and Cancer Biology at Duke University. Thiele is senior author of a study published March 13, 2013, in the journal Cell Host & Microbe. "With this research we may be closer to understanding how this fungal pathogen evades death in its host, and hopefully be closer to finding effective treatments."
Found in the environment, C. neoformans spores can be inhaled and cause infection, particularly when people have weakened immune systems. The Centers for Disease Control and Prevention estimates that worldwide, C. neoformans causes 1 million cases of meningitis a year among HIV/AIDS patients, with nearly 625,000 deaths.
Thiele and colleagues focused on the interplay between C. neoformans in the lungs of mice and the host's immune system, which mount an immediate attack against the pathogen.
The immune response is led by macrophages, which circulate in the blood stream and engulf invading microbes to destroy them. The macrophages are essentially tiny torture chambers for pathogens, using hostile conditions and toxic substances to kill invaders.
Among the substances inside the macrophages is copper, a mineral the body needs for normal cognitive function and development, but also known to have antifungal properties. In the face of a pathogenic invasion of fungal spores, the macrophages begin concentrating more copper within their torture chamber as one of the body's antifungal weapons.
The Duke researchers found that lethal strains of C. neoformans have two ways of battling against the toxicity of the copper. First, the pathogen turns on genes that make proteins to protect it from copper toxicity, so even when exposed to the hostile copper environment in the macrophages, it survives.
But a second defense mechanism is also deployed. The fungus, sensing the copper-rich environment, triggers a response that shuts down the host's ability to pump more copper into the macrophages – defusing this weapon in the immune system's arsenal.
"With these two mechanisms, C. neoformans can defend itself by sequestering the copper, and somehow communicate to the host macrophage, commanding that it shut down the copper pumps," Thiele said.
Thiele said studies are now focusing on how antifungal agents might thwart the pathogen's two defense systems. "The detoxification machinery might represent an effective drug target," Thiele said.
In addition to Thiele, study authors include Chen Ding, Richard A. Festa, Ying-Lien Chen and Joseph Heitman from Duke; Anna Espart and Sílvia Atrian from Universitat de Barcelona, Spain; Òscar Palacios, Jordi Espín and Mercè Capdevila from Universitat Autònoma de Barcelona, Spain.
The work was supported in part by the National Institutes of Health (GM48140-24, 2P30 AI064518-06, AI50438).
Sarah Avery | EurekAlert!
Good preparation is half the digestion
15.11.2018 | Max-Planck-Institut für Stoffwechselforschung
How the gut ‘talks’ to brown fat
16.11.2018 | Technische Universität München
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly
The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
15.11.2018 | Earth Sciences
15.11.2018 | Physics and Astronomy
15.11.2018 | Physics and Astronomy