Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Embryonic development: How do limbs develop from cells?

22.05.2018

New microscopy software enables precise monitoring of cells

During the development of a multicell organism, a single cell produces a range of different cell types which in turn form various tissues and organs.


The developmental sequence of Parhyale hawaiensis captured

using alight screen fluorescence microscope. This concerns 3D

reconstructions of an embryo from a lateral view and the head

on the left side. The areas highlighted in colourshow how legs differ

on the basis of just a small number of cells.

Copyright owners: Anastasios Pavlopoulos and Carsten Wolff

For over a century, biologists have been attempting to understand the biogenesis (also known as cell fate) of the different structures in developing organisms. For instance, which cells produce eyes or limbs? How does the behaviour of these developing cells change in order to realise the form and function of the respective organ?

Until now, it has been exceedingly difficult to answer these kinds of questions since directly observing developing embryos at the cellular level poses a considerable challenge.

Following a new approach, a multinational and interdisciplinary team of scientists from the Humboldt University of Berlin, the Max-Planck Institute for Molecular Cell Biology and Genetics (Dresden), the HHMI Janelia Research Campus (USA), the Max-Delbrück Centre for Molecular Medicine (Berlin) and the Pasteur Institute (France) is investigating the dynamics and behaviour of cells during limb development.

In this connection, the research team is taking a closer look at the development of the marine crustacean Parhyale hawaiensis. This creature has developed an array of specialised limbs along the length of its body – resembling a living Swiss army knife.

Using state-of-the-art light sheet fluorescence microscopy, the researchers were able to record the entire embryonic development of embryos with fluorescent cell nuclei. This procedure involved the embryos being observed from different perspectives in a high spatial and temporal resolution.

Cell monitoring can provide insights into deformities

Having collecting these complex and multidimensional microscopic images, the researchers faced the challenge of extracting the relevant biological information from these huge data sets. To this end, they developed a special software program called Massive Multi-View Tracker (MaMuT), which is freely available as a FIJI plug-in. This combination of microscopy and software allowed the scientists to examine all the cells of a leg during development over the course of several days.

They were thus able to discover morphogenetic behavioural patterns of the cells which contribute to the cellular organisation and growth of a leg. In the future, this new method of examining the development of multicell organisms will help to identify the cellular and molecular mechanisms that determine the fate of cells during the development of an organism. It will also undoubtedly prove useful in explaining the causes of deformities during development.

Article
https://elifesciences.org/articles/34410

Foto description
The developmental sequence of Parhyale hawaiensis captured
using alight screen fluorescence microscope. This concerns 3D
reconstructions of an embryo from a lateral view and the head
on the left side. The areas highlighted in colourshow how legs differ
on the basis of just a small number of cells.

Contact
Dr Carsten Wolff
Institute for Biology
Tel.: 030 2093-6284
carsten.wolff@rz.hu-berlin.de

Sella Christin Bargel | idw - Informationsdienst Wissenschaft

Further reports about: Biology Embryonic development of embryos embryos limbs single cell

More articles from Life Sciences:

nachricht Symbiotic upcycling: Turning “low value” compounds into biomass
25.06.2019 | Max-Planck-Institut für Marine Mikrobiologie

nachricht New Therapy Promotes Vascular Repair Following Stroke
25.06.2019 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fraunhofer IDMT demonstrates its method for acoustic quality inspection at »Sensor+Test 2019« in Nürnberg

From June 25th to 27th 2019, the Fraunhofer Institute for Digital Media Technology IDMT in Ilmenau (Germany) will be presenting a new solution for acoustic quality inspection allowing contact-free, non-destructive testing of manufactured parts and components. The method which has reached Technology Readiness Level 6 already, is currently being successfully tested in practical use together with a number of industrial partners.

Reducing machine downtime, manufacturing defects, and excessive scrap

Im Focus: Successfully Tested in Praxis: Bidirectional Sensor Technology Optimizes Laser Material Deposition

The quality of additively manufactured components depends not only on the manufacturing process, but also on the inline process control. The process control ensures a reliable coating process because it detects deviations from the target geometry immediately. At LASER World of PHOTONICS 2019, the Fraunhofer Institute for Laser Technology ILT will be demonstrating how well bi-directional sensor technology can already be used for Laser Material Deposition (LMD) in combination with commercial optics at booth A2.431.

Fraunhofer ILT has been developing optical sensor technology specifically for production measurement technology for around 10 years. In particular, its »bd-1«...

Im Focus: The hidden structure of the periodic system

The well-known representation of chemical elements is just one example of how objects can be arranged and classified

The periodic table of elements that most chemistry books depict is only one special case. This tabular overview of the chemical elements, which goes back to...

Im Focus: MPSD team discovers light-induced ferroelectricity in strontium titanate

Light can be used not only to measure materials’ properties, but also to change them. Especially interesting are those cases in which the function of a material can be modified, such as its ability to conduct electricity or to store information in its magnetic state. A team led by Andrea Cavalleri from the Max Planck Institute for the Structure and Dynamics of Matter in Hamburg used terahertz frequency light pulses to transform a non-ferroelectric material into a ferroelectric one.

Ferroelectricity is a state in which the constituent lattice “looks” in one specific direction, forming a macroscopic electrical polarisation. The ability to...

Im Focus: Determining the Earth’s gravity field more accurately than ever before

Researchers at TU Graz calculate the most accurate gravity field determination of the Earth using 1.16 billion satellite measurements. This yields valuable knowledge for climate research.

The Earth’s gravity fluctuates from place to place. Geodesists use this phenomenon to observe geodynamic and climatological processes. Using...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on UV LED Technologies & Applications – ICULTA 2020 | Call for Abstracts

24.06.2019 | Event News

SEMANTiCS 2019 brings together industry leaders and data scientists in Karlsruhe

29.04.2019 | Event News

Revered mathematicians and computer scientists converge with 200 young researchers in Heidelberg!

17.04.2019 | Event News

 
Latest News

Cooling with the sun

25.06.2019 | Power and Electrical Engineering

Robocabs: The mobility of the future?

25.06.2019 | Studies and Analyses

Skipping Meat on Occasion May Protect Against Type 2 Diabetes

25.06.2019 | Health and Medicine

VideoLinks
Science & Research
Overview of more VideoLinks >>>