The study is published in a recent special issue of Cell Medicine [3(1)], now freely available on-line at: http://www.ingentaconnect.com/content/cog/cm.
"iPS cells are a promising alternative to embryonic stem cells and can be used in place of bone marrow cells, stromal cells and adipose tissue-derived stem cells," said study co-author Hirofumi Noguchi, MD, PhD, Department of Gastroenterological Surgery, Transplant and Surgical Oncology at the Okayama University Graduate School of Medicine.
"However, the viability of human iPS cells, like embryonic stem cells, decreases significantly during cryopreservation. A wide variety of cryopreservation solutions have been used, however many are toxic or ineffective for use in extended cryopreservation."
The researchers concluded that Cell Banker 3 showed the highest cell viability and proliferation of all the solutions examined and can be widely used as it does not require any special skills for use.
This research was among those studies presented at the 37th Annual Meeting of the Japan Society for Organ Preservation and Medical Biology (JSOPMB). Sixteen studies were published in this special issue of CELL MEDICINE. The theme of the issue is "Organ/Cell Transplantation and Regenerative Medicine."
Citation: Miyamoto, Y.; Noguchi, H.; Yukawa, H.; Oishi, K.; Matsushita, K.; Iwata, H.; Hayashi, S. Cryopreservation of Induced Pluripotent Stem Cells. Cell Med. 3(1):89-95; 2012.
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