Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Chemists concoct new agents to easily study critical cell proteins

01.11.2010
They are the portals to the cell, gateways through which critical signals and chemicals are exchanged between living cells and their environments.

But these gateways -- proteins that span the cell membrane and connect the world outside the cell to its vital inner workings – remain, for the most part, black boxes with little known about their structures and how they work.

They are of intense interest to scientists as they are the targets on which many drugs act, but are notoriously difficult to study because extracting these proteins intact from cell membranes is tricky.

Now, however, a team of scientists from the University of Wisconsin-Madison and Stanford University has devised a technology to more easily obtain membrane proteins for study. Writing this week (Oct. 31) in the journal Nature Methods, the group reports the development of a class of agents capable of extracting complex membrane proteins without distorting their shape, a key to understanding how they work.

"The proteins are embedded in the membrane to control what gets into the cell and what gets out," explains Samuel Gellman, a UW-Madison professor of chemistry and a senior author of the paper along with Brian Kobilka of Stanford and Bernadette Byrne of Imperial College London. "If we want to understand life at the molecular level, we need to understand the properties and functions of these membrane proteins."

The catch with membrane proteins and unleashing their potential, however, is getting insight into their physical properties, says Gellman.

Like other kinds of proteins, membrane proteins exhibit a complex pattern of folding, and determining the three-dimensional shapes they assume in the membrane provides essential insight into how they do business.

Proteins are workhorse molecules in any organism, and myriad proteins are known. Structures have been solved for many thousands of so-called "soluble" proteins, but only a couple of hundred membrane protein structures are known, Gellman notes. This contrast is important because roughly one-third of the proteins encoded in the human genome appear to be membrane proteins.

To effectively study a protein, scientists must have access to it. A primary obstacle has been simply getting proteins out of the membrane while maintaining their functional shapes. To that end, Gellman's group has developed a family of new chemical agents, known as amphiphiles, that are easily prepared, customizable to specific proteins and cheap.

"These amphiphiles are very simple," says Gellman. "That's one of their charms. The other is that they can be tuned to pull out many different kinds of proteins."

The hope, according to Gellman, is that the new technology will facilitate research at the biomedical frontier.

The development of the amphiphiles was conducted in close collaboration with groups like Kobilka's, which specializes in techniques that help resolve the three-dimensional structures of proteins found in cell membranes.

The lead author of the new study is Pil Seok Chae, a postdoctoral fellow in Gellman's lab. The work was supported primarily by the U.S National Institutes of Health.

-- Terry Devitt, 608-262-8282, trdevitt@wisc.edu

Samuel Gellman | EurekAlert!
Further information:
http://www.wisc.edu

More articles from Life Sciences:

nachricht Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View
22.06.2018 | University of Sussex

nachricht New cellular pathway helps explain how inflammation leads to artery disease
22.06.2018 | Cedars-Sinai Medical Center

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Graphene assembled film shows higher thermal conductivity than graphite film

22.06.2018 | Materials Sciences

Fast rising bedrock below West Antarctica reveals an extremely fluid Earth mantle

22.06.2018 | Earth Sciences

Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View

22.06.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>