Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Carnegie Mellon develops bio-mimicry method for preparing and labeling stem cells

18.05.2016

Method allows researchers to prepare mesenchymal stem cells and monitor them using MRI

Researchers led by Carnegie Mellon University Professor of Biological Sciences Chien Ho have developed a new method for preparing mesenchymal stem cells (MSCs) that not only leads to the production of more native stem cells, but also labels them with a FDA approved iron-oxide nanoparticle (Ferumoxytol).


Mesenchymal stem cells have labeled with Ferumoxytol using a bio-mimicry method developed at Carnegie Mellon University. The method can be used to better evaluate stem cell therapies in preclinical and clinical trials.

Credit: Carnegie Mellon University

The technology could allow researchers to track the cells in vivo using magnetic resonance imaging (MRI) during preclinical and clinical trials. The findings are published by Scientific Reports.

Stem cells, with their ability to regenerate into a multitude of different cell types, show great promise for treating a number of diseases and injuries. Stem cells taken from a patient's own body are of particular interest, due to a decreased chance of rejection. These cells are most commonly harvested from the bone marrow, which contains two types of stem cells, hematopoietic and mesenchymal.

... more about:
»Carnegie »FDA »MSCs »bone marrow »nanoparticle »stem cells

Hematopoietic stem cells can be used to form the different types of blood cells and are used to treat leukemia and multiple myeloma. Mesenchymal stem cells can be used to generate bone, cartilage and fat cells, and have promise for repairing bone and cartilage, damaged heart cells, and treating inflammatory and autoimmune diseases.

More than 360 registered clinical trials are using MSCs, but the results have been mixed, with some patients reacting well and others not responding to the stem cell treatment. To understand why these results can be so variable, researchers need to be able to track the stem cells as they migrate through the body to see if they reach and graft to the appropriate site. To do this, researchers could label the stem cells with a superparamagnetic iron-oxide (SPIO) contrast agent and image the patient using MRI.

Ferumoxytol is the only SPIO nanoparticle that has been approved by the FDA, but researchers have not been able to label MSCs with Ferumoxytol in cell culture (ex vivo) without the help of a transfection agent. Transfection agents are undesirable because they can change the cells' biology and inhibit their effectiveness. Furthermore, researchers have had difficulty culturing the large amount of cells needed for clinical dosing. Current methods also result in cells of different sizes and functionalities. Smaller, round cells are preferable because they show a higher capacity for regeneration and differentiation.

To surmount these problems, Ho and colleagues took advantage of the cell's natural ability to engulf and internalize Ferumoxytol in vivo. Ho's team developed a "bio-mimicry" method to create an environment in a petri dish that is much like the environment found inside the body. His team began by using traditional methods to extract cells from bone marrow, separate the MSCs from the other cells and expand the number of MSCs. His team then devised a new way to culture MSCs by introducing other cells from the bone marrow, mimicking the in-vivo environment. The resulting MSCs retain their optimal size and regeneration capabilities and can internalize Ferumoxytol for cell tracking. Because MSCs are multi-potent, this new methodology can prepare more native cells for applications in cell therapy and regenerative medicine.

###

Additional study authors include Li Liu, Lanya Tseng, Qing Ye and Yijen Wu from Carnegie Mellon's Department of Biological Sciences, and Daniel J. Bain from the Department of Geology and Environmental Science at the University of Pittsburgh. The research was funded by the National Institutes of Health's National Institute of Biomedical Imaging and Bioengineering (EB-001977).

Media Contact

Jocelyn Duffy
jhduffy@andrew.cmu.edu
412-268-9982

 @CMUScience

http://www.cmu.edu 

Jocelyn Duffy | EurekAlert!

Further reports about: Carnegie FDA MSCs bone marrow nanoparticle stem cells

More articles from Life Sciences:

nachricht Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg

nachricht Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: UNH scientists help provide first-ever views of elusive energy explosion

Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.

Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Purdue cancer identity technology makes it easier to find a tumor's 'address'

16.11.2018 | Health and Medicine

Good preparation is half the digestion

16.11.2018 | Life Sciences

Microscope measures muscle weakness

16.11.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>