A miniaturized device that allows wireless detection of glucose and fructose molecules typically characterized in fermentation processes can also be used to study metabolites and disease
Electrochemical reactions that occur in processes like ethanolic fermentation include the transformation of sugars into alcohol and carbon dioxide. Similar processes occur when the human body breaks down food, drugs or other compounds.
Monitoring these metabolic processes helps in testing, studying and combating disease, but due to the small quantities of liquids involved, they are difficult to study with normal equipment.
Miniaturizing devices with environmental sensing capabilities offers promise for better testing of fermentation and other biochemical processes. The ability to operate the devices wirelessly over long periods of time increases their effectiveness.
In a paper published this week in Review of Scientific Instruments, from AIP Publishing, a miniaturized potentiostat, which controls voltage between electrodes, proved capable of quantifying molecules using voltammetric and chronoamperometric methods with an accuracy above 98%. The wireless device is compatible with most 3-electrode biosensors and can transmit its measurements via Bluetooth for 100 meters.
"One of the novelties of the potentiostat circuit is to be able to process six sensors channels simultaneously without the use of multiplexers, thereby reducing the time spent on each examination," author Saad Abdullah said. "This multichannel potentiostat can examine multiple samples of different concentrations simultaneously and transfer the data over Bluetooth in real time."
The potentiostat was tested to confirm its performance under fixed resistance and quantify the current detection limits and noise in the system, as well as its accuracy and response time. Experiments showed a current detection limit of 180 nanoamperes and a plus or minus 2% standard deviation in cyclic voltammetry measurement. They also conducted an experimental test involving six different concentrations of glucose using a chronoamperometry technique.
In the experiment, the potentiostat was equipped with screen-printed electrodes modified with the enzyme glucose oxidase, which binds with the target protein and acts as an electrochemical channel between the protein and the sensor chip. When a chronoamperometric signal is applied to the sensor chip, an output current is observed in the potentiostat that is equivalent to the concentration of glucose in the sample.
The potentiostat proved capable of operating independently and transmitting data wirelessly for 24 hours in an incubator with accuracy comparable to commercially available devices. The wireless system produced a clear data signal, 180 times stronger than the noise in the circuit. This online method has the added advantage over currently available devices that data from six different biosensors can be viewed simultaneously in real time on an external monitor.
The article, "Design of multichannel potentiostat for remote and longtime monitoring of glucose concentration during yeast fermentation," is authored by Saad Abdullah, Mauro Serpelloni and Emilio Sardini. The article will appear in Review of Scientific Instruments on May 5, 2020 (DOI: 10.1063/1.5137789). After that date, it can be accessed at https:/
ABOUT THE JOURNAL
Review of Scientific Instruments publishes novel advancements in scientific instrumentation, apparatuses, techniques of experimental measurement, and related mathematical analysis. Its content includes publication on instruments covering all areas of science including physics, chemistry, materials science, and biology. See https:/
Larry Frum | EurekAlert!
Polarization of Br2 molecule in vanadium oxide cluster cavity and new alkane bromination
13.07.2020 | Kanazawa University
Researchers present concept for a new technique to study superheavy elements
13.07.2020 | Johannes Gutenberg-Universität Mainz
Biochemists at Martin Luther University Halle-Wittenberg (MLU) have used a standard electron cryo-microscope to achieve surprisingly good images that are on par with those taken by far more sophisticated equipment. They have succeeded in determining the structure of ferritin almost at the atomic level. Their results were published in the journal "PLOS ONE".
Electron cryo-microscopy has become increasingly important in recent years, especially in shedding light on protein structures. The developers of the new...
New insight into the spin behavior in an exotic state of matter puts us closer to next-generation spintronic devices
Aside from the deep understanding of the natural world that quantum physics theory offers, scientists worldwide are working tirelessly to bring forth a...
Kiel physics team observed extremely fast electronic changes in real time in a special material class
In physics, they are currently the subject of intensive research; in electronics, they could enable completely new functions. So-called topological materials...
Solar cells based on perovskite compounds could soon make electricity generation from sunlight even more efficient and cheaper. The laboratory efficiency of these perovskite solar cells already exceeds that of the well-known silicon solar cells. An international team led by Stefan Weber from the Max Planck Institute for Polymer Research (MPI-P) in Mainz has found microscopic structures in perovskite crystals that can guide the charge transport in the solar cell. Clever alignment of these "electron highways" could make perovskite solar cells even more powerful.
Solar cells convert sunlight into electricity. During this process, the electrons of the material inside the cell absorb the energy of the light....
Empa researchers have succeeded in applying aerogels to microelectronics: Aerogels based on cellulose nanofibers can effectively shield electromagnetic radiation over a wide frequency range – and they are unrivalled in terms of weight.
Electric motors and electronic devices generate electromagnetic fields that sometimes have to be shielded in order not to affect neighboring electronic...
07.07.2020 | Event News
02.07.2020 | Event News
19.05.2020 | Event News
13.07.2020 | Physics and Astronomy
13.07.2020 | Life Sciences
13.07.2020 | Life Sciences