By studying fish raised in aquaculture, researchers from the Helmholtz Zentrum München, the University of Copenhagen and the University of Campinas in Brazil have shed new light on the mechanisms by which antibiotic resistance genes are transferred between bacteria. According to the study published in the journal ‘Microbiome’, those mechanisms are more varied than previously thought.
“In the past 70 years, the use of antibiotics in human and veterinary medicine has steadily increased, leading to a dramatic rise in resistant microorganisms,” says Prof. Dr. Michael Schloter, head of the Research Unit for Comparative Microbiome Analyses (COMI) at Helmholtz Zentrum München.
It is especially alarming that many microorganisms are resistant not just to one antibiotic, but to a whole range of different substances, states the corresponding author of the recent study.
This poses particular problems in the treatment of infectious diseases. “We therefore set out to discover the mechanisms responsible for resistance development,” he says.
To this end, he and his team, together with Danish scientists led by Gisle Vestergaard (University of Copenhagen and Helmholtz Zentrum München), investigated fish raised in aquaculture. Specifically, they studied Piaractus mesopotamicus, a South American species known as pacu that is often raised in aquaculture.
The fish received the antibiotic florfenicol in their food for 34 days. During this time and after the application period, the researchers took samples from the digestive tract of the fish and looked for relevant genetic changes in the gut bacteria.
Resistance genes hop around the genome
“As expected, administration of the antibiotic induced an increase in the genes responsible for resistance to that antibiotic,” explains COMI doctoral student Johan Sebastian Sáenz Medina, lead author of the paper. “One example are genes for pump proteins, which simply remove the active substance from the bacteria again. However, we were particularly surprised by the different mechanisms that we could detect by which antibiotic resistance genes are spread amongst gut bacteria of the fish” Sáenz Medina explains. “This suggests that the bacteria also exchange resistance through viruses, known as phages, and transposons.”
Further metagenomic studies confirmed that these mobile genetic elements induce a fast distribution of resistance genes among genomes of different organisms. So far it has been postulated that only plasmids (in essence, easily transferable mini-chromosomes) are mainly responsible for the exchange of resistance genes.
“The finding that resistance is also extensively transferred between bacteria without the involvement of plasmids is really quite surprising,” says Michael Schloter. “Based on this observation, relevant dissemination models should be reviewed and modified. In addition, our data certainly lead us to question whether and to what extent we should continue to use antibiotics in the world’s increasing number of aquacultures.”
At the same time, the antibiotic also changed the composition of the bacteria in the digestive tract of the fish. Putatively pathogenic genera such as Salmonella, Plesiomonas and Citrobacter proliferated. However, once the experiment ended, the microbiome returned to its original composition.
Michael Schloter has been dedicating his research to antibiotic resistance for some time. Recently, he was involved in a study in 'Environment International' that showed how pollution from antibiotic production induces spatial and seasonal bacterial community shifts in receiving river sediments. https://www.sciencedirect.com/science/article/pii/S0160412018322803?via%3Dihub
The Helmholtz Zentrum München, the German Research Center for Environmental Health, pursues the goal of developing personalized medical approaches for the prevention and therapy of major common diseases such as diabetes, allergies and lung diseases. To achieve this, it investigates the interaction of genetics, environmental factors and lifestyle. The Helmholtz Zentrum München is headquartered in Neuherberg in the north of Munich and has about 2,300 staff members. It is a member of the Helmholtz Association, a community of 18 scientific-technical and medical-biological research centers with a total of about 37,000 staff members. http://www.helmholtz-muenchen.de/en
The Research Unit for Comparative Microbiome Analyses (COMI) strives to elucidate elementary modes of action in microbiome development and the associated formation of networks which occur independently from any particular environment and can thus be considered as general principles of microbe – microbe interactions. A major issue will be to improve our understanding on the role of microbial networks for stability and resilience towards stressors or changing environmental conditions. This focus of research will not only unravel microbial functions in different environments, where microbes play an essential role, but it will also improve our in-depth understanding of microbiome interactions from different environments. Further results will allow the development of tools to restore microbiomes and thus improving the health of the hosts (contributing to the “red” and the “green” research fields of HMGU. http://www.helmholtz-muenchen.de/comi
Contact for the media:
Communication Department, Helmholtz Zentrum München - German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg - Tel. +49 89 3187 2238 - E-mail: firstname.lastname@example.org
Prof. Dr. Michael Schloter, Helmholtz Zentrum München - German Research Center for Environmental Health, Research Unit for Comparative Microbiome Analyses, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany - Tel. +49 89 3187 2304 - E-mail: email@example.com
Sáenz, J.S. et al. (2019): Oral administration of antibiotics increased the potential mobility of bacterial resistance genes in the gut of the fish Piaractus mesopotamicus. Microbiome, DOI: 10.1186/s40168-019-0632-7
Sonja Opitz | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Münster University chemists create new types of Lewis acids on the basis of phosphorus
22.10.2019 | Westfälische Wilhelms-Universität Münster
Obesity risk quantification:a jump towards the future through the artificial intelligence lens applied to lipid science
22.10.2019 | Technische Universität Dresden
Researchers have succeeded in creating an efficient quantum-mechanical light-matter interface using a microscopic cavity. Within this cavity, a single photon is emitted and absorbed up to 10 times by an artificial atom. This opens up new prospects for quantum technology, report physicists at the University of Basel and Ruhr-University Bochum in the journal Nature.
Quantum physics describes photons as light particles. Achieving an interaction between a single photon and a single atom is a huge challenge due to the tiny...
A very special kind of light is emitted by tungsten diselenide layers. The reason for this has been unclear. Now an explanation has been found at TU Wien (Vienna)
It is an exotic phenomenon that nobody was able to explain for years: when energy is supplied to a thin layer of the material tungsten diselenide, it begins to...
Researchers at Ludwig-Maximilians-Universitaet (LMU) in Munich have explored the initial consequences of the interaction of light with molecules on the surface of nanoscopic aerosols.
The nanocosmos is constantly in motion. All natural processes are ultimately determined by the interplay between radiation and matter. Light strikes particles...
Particles that are mere nanometers in size are at the forefront of scientific research today. They come in many different shapes: rods, spheres, cubes, vesicles, S-shaped worms and even donut-like rings. What makes them worthy of scientific study is that, being so tiny, they exhibit quantum mechanical properties not possible with larger objects.
Researchers at the Center for Nanoscale Materials (CNM), a U.S. Department of Energy (DOE) Office of Science User Facility located at DOE's Argonne National...
A new research project at the TH Mittelhessen focusses on the development of a novel light weight design concept for leisure boats and yachts. Professor Stephan Marzi from the THM Institute of Mechanics and Materials collaborates with Krake Catamarane, which is a shipyard located in Apolda, Thuringia.
The project is set up in an international cooperation with Professor Anders Biel from Karlstad University in Sweden and the Swedish company Lamera from...
02.10.2019 | Event News
02.10.2019 | Event News
19.09.2019 | Event News
22.10.2019 | Materials Sciences
22.10.2019 | Medical Engineering
22.10.2019 | Power and Electrical Engineering