Antibiotic resistant bacteria lead to infections that are difficult to treat, particularly in hospitals. Multidrug-resistant Escherichia coli bacteria which have developed special enzymes that render antibiotics ineffective frequently cause such infections. DZIF scientists from Gießen University examined these bacteria more precisely and discovered a strain of Escherichia coli that has been spreading rapidly in Germany since 2010 and is insensitive to several antibiotics at once.
Escherichia coli, or E. coli, is a member of a group of Gram-negative Enterobacteriaceae that predominantly reside in the human gut. Some strains can trigger infections when they access other regions of the body. In weakened patients in particular, they cause blood stream, wound and urinary tract infections.
Their treatment is becoming increasingly challenging: in the fight against antibiotics E. coli bacteria and other Enterobacteriaceae have developed enzymes, so-called extended-spectrum beta-lactamases (ESBL), which render antibiotics ineffective. This mechanism of defence makes the bacterial pathogens multidrug-resistant and they are particularly dreaded in hospitals.
“We need to pay particular attention to a subset of multidrug-resistant E. coli bacteria which we have found in our current study,” explains Prof Trinad Chakraborty, Director of the Institute for Medical Microbiology at the JLU in Gießen and Coordinator at the DZIF partner site Gießen-Marburg-Langen. This subset is currently spreading globally and has now also been found in Germany.
Genome sequencing for monitoring antibiotic resistance
In the study, the scientists from Gießen investigated almost 1000 isolates of ESBL-producing bacteria from humans, animals, the environment and food. Their approach is in-line with the One Health approach which not only includes humans but also the respective environment in investigations.
They specifically identified beta-lactamase genes and searched for a subset which had already increasing in other countries: a multidrug-resistant strain of E. coli, sequence type 131 (ST131). This strain is responsible for millions of infections worldwide, particularly for blood stream and bladder infections, and carries a relatively rare ESBL gene called blaCTX-M-27.
The quest was successful: the researchers found E. coli ST131 CTX-M27 to be exclusively present in the human isolates and could confirm that its incidence had increased from 0 % in 2009 to 45 % in 2016.
“With this, this E. coli strain and its specific ESBL gene has become a competitor for an E. coli ST131 strain carrying a different ESBL gene, which has been the most prevalent in Germany up to now,” explains Dr Can Imirzalioglu, scientist at the Gießen University. More studies are required to investigate the causes and clinical implications of this shift. However, the results highlight the importance of modern methods such as genome sequencing for observing developments like this and for reacting rapidly in emergencies.
This project was supported by the DZIF and the RESET Research Network which is dedicated to investigating antibiotic resistance in Enterobacteriaceae. Since 2013, scientists from the DZIF research field “Healthcare-associated and Antibiotic-resistant bacterial Infections” have been working together closely with the RESET Research Network in Gießen to collect multidrug-resistant infectious disease pathogens from animals and humans. Bacterial genomes from the isolates in this extensive collection were sequenced at the DZIF’s Bioinformatics Department and stored in a database. This genome database has repeatedly been used to successfully investigate the occurrence of resistance more closely.
Hiren Ghosh, Swapnil Doijad, Linda Falgenhauer, Moritz Fritzenwanker, Can Imirzalioglu, and Trinad Chakraborty: blaCTX-M-27–Encoding Escherichia coli Sequence Type 131 Lineage C1-M27 Clone in Clinical Isolates, Germany
Emerging Infectious Diseases Volume 23, Number 10—October 2017
Prof Trinad Chakraborty
DZIF research field “Healthcare-associated and Antibiotic-resistant bacterial Infections”
Institute for Medical Microbiology
Justus Liebig University Gießen
T: +49 641 99 41251/81
Janna Schmidt and Karola Neubert
German Center for Infection Research
T: +49 531 6181 1154/1170
Karola Neubert | idw - Informationsdienst Wissenschaft
Colorectal cancer risk factors decrypted
13.07.2018 | Max-Planck-Institut für Stoffwechselforschung
Algae Have Land Genes
13.07.2018 | Julius-Maximilians-Universität Würzburg
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
13.07.2018 | Event News
13.07.2018 | Materials Sciences
13.07.2018 | Life Sciences