CNMPB scientists develop new tool to analyze cellular structures via high- resolution imaging. Published May 26th, 2014 in the Journal of Cell Biology.
In the past two decades, super-resolution microscopy has been one of the fastest evolving fields through many technical improvements. However, the development of new labeling tools, probes and their biological application, is mostly lagging behind the technical capabilities.
From left to right: Prof. Dr. Silvio O. Rizzoli, Natalia Revelo, Dr. Dirk Kamin, Sven Truckenbrodt.
Most recently, Prof. Silvio O. Rizzoli from the Cluster of Excellence and DFG- Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) has developed together with his team a new technique that expands the benefit of super- resolution microscopy to study biological questions.
This method contributes to understand on how cells renew, distribute and transport their molecular and subcellular components. The new technique was published on May 26th in the Journal of Cell Biology.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter K, Reisinger E, Moser T, Rizzoli SO (2014) A new probe for super-resolution imaging of membranes elucidates trafficking pathways. J CELL BIOL, May 26; 205(4): 591-606.
All cells rely on the recycling of membranes via various pathways (secretion, uptake, and membrane turnover). Several types of cellular organelles such as the plasma membrane, the endoplasmic reticulum, the Golgi apparatus, endosomes and vesicles are involved in these processes.
However, it was difficult to identify the protein composition of the involved organelles since both, the membranes and the proteins of the same organelle need to be marked simultaneously. Here the main difficulty comes with the membrane probe, as almost all dyes that work excellent in live cell experiments are only poorly fixable and get “lost” during the antibody staining procedure.
The research team with first author Natalia Revelo therefore developed a membrane probe that overcomes this problem. The probe mCLING (membrane-binding fluorophore-Cysteine- Lysine-Palmitoyl Group) is a composition of a short polypeptide coupled to a membrane anchor and a fluorophore.
The study, recently published in the Journal of Cell Biology, shows that mCLING can be used to label the plasma membrane, and also to faithfully track specific organelles, which can be done in conjunction with fixation and immunostaining, in both cell culture and in tissue.
The utility of the mCLING probe could be characterized for various important biological model systems and already enabled the authors to answer long-lasting question in the field of membrane recycling. Moreover, mCLING imaging could also be extended to different processes.
For example, the structure and molecular organization of isolated organelles in vitro, or the arrangement of proteins on the membranes of various types of cells, can be easily tackled with mCLING. These efforts will be aided by the fact that mCLING can be optimized for any available super-resolution technique.
Prof. Dr. Silvio O. Rizzoli is head of the Department of Neuro- and Sensory Physiology at the University Medical Center Göttingen and member of the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB). His research focus includes the identification of molecular signal transduction processes between nerve cells.
Prof. Rizzoli applies super-resolution microscopy techniques to study the transport and function of intracellular vesicles in the synapses of nerve cells. Very recently, Prof. Rizzoli received for the second time with a prestigious funding award of the European Union for his excellent research proposal.
Prof. Dr. Silvio O. Rizzoli
University Medical Center Göttingen Department Neuro- & Sensory Physiology
c/o European Neuroscience Institute (ENI) Grisebachstraße 5, 37077 Göttingen
Phone 0551 / 39-33630, email@example.com
CNMPB – Center for Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 – DFG Research Center 103
Dr. Heike Conrad
Scientific Coordination, Press & Public Relations
Humboldtallee 23, 37073 Göttingen
Phone 0551 / 39-7065, firstname.lastname@example.org
Dr. Heike Conrad | idw - Informationsdienst Wissenschaft
Colorectal cancer risk factors decrypted
13.07.2018 | Max-Planck-Institut für Stoffwechselforschung
Algae Have Land Genes
13.07.2018 | Julius-Maximilians-Universität Würzburg
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
13.07.2018 | Event News
13.07.2018 | Materials Sciences
13.07.2018 | Life Sciences